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Arbuscular Mycorrhizal Fungi and Plant Chemical Defence: Effects of Colonisation on Aboveground and Belowground Metabolomes

Arbuscular mycorrhizal fungal (AMF) colonisation of plant roots is one of the most ancient and widespread interactions in ecology, yet the systemic consequences for plant secondary chemistry remain unclear. We performed the first metabolomic investigation into the impact of AMF colonisation by Rhizo...

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Autores principales: Hill, Elizabeth M., Robinson, Lynne A., Abdul-Sada, Ali, Vanbergen, Adam J., Hodge, Angela, Hartley, Sue E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843688/
https://www.ncbi.nlm.nih.gov/pubmed/29392532
http://dx.doi.org/10.1007/s10886-017-0921-1
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author Hill, Elizabeth M.
Robinson, Lynne A.
Abdul-Sada, Ali
Vanbergen, Adam J.
Hodge, Angela
Hartley, Sue E.
author_facet Hill, Elizabeth M.
Robinson, Lynne A.
Abdul-Sada, Ali
Vanbergen, Adam J.
Hodge, Angela
Hartley, Sue E.
author_sort Hill, Elizabeth M.
collection PubMed
description Arbuscular mycorrhizal fungal (AMF) colonisation of plant roots is one of the most ancient and widespread interactions in ecology, yet the systemic consequences for plant secondary chemistry remain unclear. We performed the first metabolomic investigation into the impact of AMF colonisation by Rhizophagus irregularis on the chemical defences, spanning above- and below-ground tissues, in its host-plant ragwort (Senecio jacobaea). We used a non-targeted metabolomics approach to profile, and where possible identify, compounds induced by AMF colonisation in both roots and shoots. Metabolomics analyses revealed that 33 compounds were significantly increased in the root tissue of AMF colonised plants, including seven blumenols, plant-derived compounds known to be associated with AMF colonisation. One of these was a novel structure conjugated with a malonyl-sugar and uronic acid moiety, hitherto an unreported combination. Such structural modifications of blumenols could be significant for their previously reported functional roles associated with the establishment and maintenance of AM colonisation. Pyrrolizidine alkaloids (PAs), key anti-herbivore defence compounds in ragwort, dominated the metabolomic profiles of root and shoot extracts. Analyses of the metabolomic profiles revealed an increase in four PAs in roots (but not shoots) of AMF colonised plants, with the potential to protect colonised plants from below-ground organisms. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10886-017-0921-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-58436882018-03-19 Arbuscular Mycorrhizal Fungi and Plant Chemical Defence: Effects of Colonisation on Aboveground and Belowground Metabolomes Hill, Elizabeth M. Robinson, Lynne A. Abdul-Sada, Ali Vanbergen, Adam J. Hodge, Angela Hartley, Sue E. J Chem Ecol Article Arbuscular mycorrhizal fungal (AMF) colonisation of plant roots is one of the most ancient and widespread interactions in ecology, yet the systemic consequences for plant secondary chemistry remain unclear. We performed the first metabolomic investigation into the impact of AMF colonisation by Rhizophagus irregularis on the chemical defences, spanning above- and below-ground tissues, in its host-plant ragwort (Senecio jacobaea). We used a non-targeted metabolomics approach to profile, and where possible identify, compounds induced by AMF colonisation in both roots and shoots. Metabolomics analyses revealed that 33 compounds were significantly increased in the root tissue of AMF colonised plants, including seven blumenols, plant-derived compounds known to be associated with AMF colonisation. One of these was a novel structure conjugated with a malonyl-sugar and uronic acid moiety, hitherto an unreported combination. Such structural modifications of blumenols could be significant for their previously reported functional roles associated with the establishment and maintenance of AM colonisation. Pyrrolizidine alkaloids (PAs), key anti-herbivore defence compounds in ragwort, dominated the metabolomic profiles of root and shoot extracts. Analyses of the metabolomic profiles revealed an increase in four PAs in roots (but not shoots) of AMF colonised plants, with the potential to protect colonised plants from below-ground organisms. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10886-017-0921-1) contains supplementary material, which is available to authorized users. Springer US 2018-02-02 2018 /pmc/articles/PMC5843688/ /pubmed/29392532 http://dx.doi.org/10.1007/s10886-017-0921-1 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Hill, Elizabeth M.
Robinson, Lynne A.
Abdul-Sada, Ali
Vanbergen, Adam J.
Hodge, Angela
Hartley, Sue E.
Arbuscular Mycorrhizal Fungi and Plant Chemical Defence: Effects of Colonisation on Aboveground and Belowground Metabolomes
title Arbuscular Mycorrhizal Fungi and Plant Chemical Defence: Effects of Colonisation on Aboveground and Belowground Metabolomes
title_full Arbuscular Mycorrhizal Fungi and Plant Chemical Defence: Effects of Colonisation on Aboveground and Belowground Metabolomes
title_fullStr Arbuscular Mycorrhizal Fungi and Plant Chemical Defence: Effects of Colonisation on Aboveground and Belowground Metabolomes
title_full_unstemmed Arbuscular Mycorrhizal Fungi and Plant Chemical Defence: Effects of Colonisation on Aboveground and Belowground Metabolomes
title_short Arbuscular Mycorrhizal Fungi and Plant Chemical Defence: Effects of Colonisation on Aboveground and Belowground Metabolomes
title_sort arbuscular mycorrhizal fungi and plant chemical defence: effects of colonisation on aboveground and belowground metabolomes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843688/
https://www.ncbi.nlm.nih.gov/pubmed/29392532
http://dx.doi.org/10.1007/s10886-017-0921-1
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