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Stability of matrix metalloproteinase-9 as biological marker in colorectal cancer
Matrix metalloproteinases (MMPs) are believed to be of importance in the growth and spread of colorectal cancer (CRC). MMP-9 level has been suggested as a biological predictor of prognosis in CRC as well as in other types of cancer such as breast and cervical cancer. The purpose of this study was to...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843695/ https://www.ncbi.nlm.nih.gov/pubmed/29520667 http://dx.doi.org/10.1007/s12032-018-1109-4 |
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author | Jonsson, Andreas Hjalmarsson, Claes Falk, Peter Ivarsson, Marie-Lois |
author_facet | Jonsson, Andreas Hjalmarsson, Claes Falk, Peter Ivarsson, Marie-Lois |
author_sort | Jonsson, Andreas |
collection | PubMed |
description | Matrix metalloproteinases (MMPs) are believed to be of importance in the growth and spread of colorectal cancer (CRC). MMP-9 level has been suggested as a biological predictor of prognosis in CRC as well as in other types of cancer such as breast and cervical cancer. The purpose of this study was to investigate the stability over time of MMP-9 in cryopreserved plasma, colorectal tumor tissue extract and macroscopically tumor-free colon mucosa tissue extract samples. Plasma and tissue samples were taken from patients at primary CRC surgery and analyzed for MMP-9. Aliquots of samples from the same patients were stored at – 80 °C pending analysis. These aliquots were analyzed using identical methods after storage periods of nine (plasma) and twelve (tissue) years. No significant difference in plasma MMP-9 concentration was seen between baseline samples and those after 9 years of cryopreservation (median values 9.9 and 9.7 ng/mL, respectively; p > 0.05). MMP-9 levels in the tumor-free tissue extracts had increased to baseline (median values 7.1 and 8.1 ng/mL, respectively; p < 0.01). MMP-9 levels in the tumor tissue extracts had also increased significantly (median values 89.9 and 133.5 ng/mL, respectively; p < 0.01). We have demonstrated that MMP-9 levels in frozen citrated plasma are stable if stored at − 80 °C, whereas MMP-9 levels in extracts from tumor tissue and tumor-free intestinal mucosa appear to increase with time. We conclude that MMP-9 levels in cryopreserved plasma may be considered stable over time and are thus suitable for comparison purposes in consecutive series. |
format | Online Article Text |
id | pubmed-5843695 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-58436952018-03-19 Stability of matrix metalloproteinase-9 as biological marker in colorectal cancer Jonsson, Andreas Hjalmarsson, Claes Falk, Peter Ivarsson, Marie-Lois Med Oncol Original Paper Matrix metalloproteinases (MMPs) are believed to be of importance in the growth and spread of colorectal cancer (CRC). MMP-9 level has been suggested as a biological predictor of prognosis in CRC as well as in other types of cancer such as breast and cervical cancer. The purpose of this study was to investigate the stability over time of MMP-9 in cryopreserved plasma, colorectal tumor tissue extract and macroscopically tumor-free colon mucosa tissue extract samples. Plasma and tissue samples were taken from patients at primary CRC surgery and analyzed for MMP-9. Aliquots of samples from the same patients were stored at – 80 °C pending analysis. These aliquots were analyzed using identical methods after storage periods of nine (plasma) and twelve (tissue) years. No significant difference in plasma MMP-9 concentration was seen between baseline samples and those after 9 years of cryopreservation (median values 9.9 and 9.7 ng/mL, respectively; p > 0.05). MMP-9 levels in the tumor-free tissue extracts had increased to baseline (median values 7.1 and 8.1 ng/mL, respectively; p < 0.01). MMP-9 levels in the tumor tissue extracts had also increased significantly (median values 89.9 and 133.5 ng/mL, respectively; p < 0.01). We have demonstrated that MMP-9 levels in frozen citrated plasma are stable if stored at − 80 °C, whereas MMP-9 levels in extracts from tumor tissue and tumor-free intestinal mucosa appear to increase with time. We conclude that MMP-9 levels in cryopreserved plasma may be considered stable over time and are thus suitable for comparison purposes in consecutive series. Springer US 2018-03-09 2018 /pmc/articles/PMC5843695/ /pubmed/29520667 http://dx.doi.org/10.1007/s12032-018-1109-4 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Paper Jonsson, Andreas Hjalmarsson, Claes Falk, Peter Ivarsson, Marie-Lois Stability of matrix metalloproteinase-9 as biological marker in colorectal cancer |
title | Stability of matrix metalloproteinase-9 as biological marker in colorectal cancer |
title_full | Stability of matrix metalloproteinase-9 as biological marker in colorectal cancer |
title_fullStr | Stability of matrix metalloproteinase-9 as biological marker in colorectal cancer |
title_full_unstemmed | Stability of matrix metalloproteinase-9 as biological marker in colorectal cancer |
title_short | Stability of matrix metalloproteinase-9 as biological marker in colorectal cancer |
title_sort | stability of matrix metalloproteinase-9 as biological marker in colorectal cancer |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843695/ https://www.ncbi.nlm.nih.gov/pubmed/29520667 http://dx.doi.org/10.1007/s12032-018-1109-4 |
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