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The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture

Mechanical stimuli obviously affect disc nucleus pulposus (NP) biology. Previous studies have indicated that static compression exhibits detrimental effects on disc biology compared with dynamic compression. To study disc NP cell senescence under static compression and dynamic compression in a disc...

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Detalles Bibliográficos
Autores principales: Shi, Jianmin, Pang, Lianglong, Jiao, Shouguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843747/
https://www.ncbi.nlm.nih.gov/pubmed/29437905
http://dx.doi.org/10.1042/BSR20180064
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author Shi, Jianmin
Pang, Lianglong
Jiao, Shouguo
author_facet Shi, Jianmin
Pang, Lianglong
Jiao, Shouguo
author_sort Shi, Jianmin
collection PubMed
description Mechanical stimuli obviously affect disc nucleus pulposus (NP) biology. Previous studies have indicated that static compression exhibits detrimental effects on disc biology compared with dynamic compression. To study disc NP cell senescence under static compression and dynamic compression in a disc organ culture, porcine discs were cultured and subjected to compression (static compression: 0.4 MPa for 4 h once per day; dynamic compression: 0.4 MPa at a frequency of 1.0 Hz for 4 h once per day) for 7 days using a self-developed mechanically active bioreactor. The non-compressed discs were used as controls. Compared with the dynamic compression, static compression significantly promoted disc NP cell senescence, reflected by the increased senescence-associated β-galactosidase (SA-β-Gal) activity, senescence-associated heterochromatic foci (SAHF) formation and senescence markers expression, and the decreased telomerase (TE) activity and NP matrix biosynthesis. Static compression accelerates disc NP cell senescence compared with the dynamic compression in a disc organ culture. The present study provides that acceleration of NP cell senescence may be involved in previously reported static compression-mediated disc NP degenerative changes.
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spelling pubmed-58437472018-03-26 The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture Shi, Jianmin Pang, Lianglong Jiao, Shouguo Biosci Rep Research Articles Mechanical stimuli obviously affect disc nucleus pulposus (NP) biology. Previous studies have indicated that static compression exhibits detrimental effects on disc biology compared with dynamic compression. To study disc NP cell senescence under static compression and dynamic compression in a disc organ culture, porcine discs were cultured and subjected to compression (static compression: 0.4 MPa for 4 h once per day; dynamic compression: 0.4 MPa at a frequency of 1.0 Hz for 4 h once per day) for 7 days using a self-developed mechanically active bioreactor. The non-compressed discs were used as controls. Compared with the dynamic compression, static compression significantly promoted disc NP cell senescence, reflected by the increased senescence-associated β-galactosidase (SA-β-Gal) activity, senescence-associated heterochromatic foci (SAHF) formation and senescence markers expression, and the decreased telomerase (TE) activity and NP matrix biosynthesis. Static compression accelerates disc NP cell senescence compared with the dynamic compression in a disc organ culture. The present study provides that acceleration of NP cell senescence may be involved in previously reported static compression-mediated disc NP degenerative changes. Portland Press Ltd. 2018-03-09 /pmc/articles/PMC5843747/ /pubmed/29437905 http://dx.doi.org/10.1042/BSR20180064 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Shi, Jianmin
Pang, Lianglong
Jiao, Shouguo
The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture
title The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture
title_full The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture
title_fullStr The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture
title_full_unstemmed The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture
title_short The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture
title_sort response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843747/
https://www.ncbi.nlm.nih.gov/pubmed/29437905
http://dx.doi.org/10.1042/BSR20180064
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