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The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture
Mechanical stimuli obviously affect disc nucleus pulposus (NP) biology. Previous studies have indicated that static compression exhibits detrimental effects on disc biology compared with dynamic compression. To study disc NP cell senescence under static compression and dynamic compression in a disc...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843747/ https://www.ncbi.nlm.nih.gov/pubmed/29437905 http://dx.doi.org/10.1042/BSR20180064 |
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author | Shi, Jianmin Pang, Lianglong Jiao, Shouguo |
author_facet | Shi, Jianmin Pang, Lianglong Jiao, Shouguo |
author_sort | Shi, Jianmin |
collection | PubMed |
description | Mechanical stimuli obviously affect disc nucleus pulposus (NP) biology. Previous studies have indicated that static compression exhibits detrimental effects on disc biology compared with dynamic compression. To study disc NP cell senescence under static compression and dynamic compression in a disc organ culture, porcine discs were cultured and subjected to compression (static compression: 0.4 MPa for 4 h once per day; dynamic compression: 0.4 MPa at a frequency of 1.0 Hz for 4 h once per day) for 7 days using a self-developed mechanically active bioreactor. The non-compressed discs were used as controls. Compared with the dynamic compression, static compression significantly promoted disc NP cell senescence, reflected by the increased senescence-associated β-galactosidase (SA-β-Gal) activity, senescence-associated heterochromatic foci (SAHF) formation and senescence markers expression, and the decreased telomerase (TE) activity and NP matrix biosynthesis. Static compression accelerates disc NP cell senescence compared with the dynamic compression in a disc organ culture. The present study provides that acceleration of NP cell senescence may be involved in previously reported static compression-mediated disc NP degenerative changes. |
format | Online Article Text |
id | pubmed-5843747 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58437472018-03-26 The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture Shi, Jianmin Pang, Lianglong Jiao, Shouguo Biosci Rep Research Articles Mechanical stimuli obviously affect disc nucleus pulposus (NP) biology. Previous studies have indicated that static compression exhibits detrimental effects on disc biology compared with dynamic compression. To study disc NP cell senescence under static compression and dynamic compression in a disc organ culture, porcine discs were cultured and subjected to compression (static compression: 0.4 MPa for 4 h once per day; dynamic compression: 0.4 MPa at a frequency of 1.0 Hz for 4 h once per day) for 7 days using a self-developed mechanically active bioreactor. The non-compressed discs were used as controls. Compared with the dynamic compression, static compression significantly promoted disc NP cell senescence, reflected by the increased senescence-associated β-galactosidase (SA-β-Gal) activity, senescence-associated heterochromatic foci (SAHF) formation and senescence markers expression, and the decreased telomerase (TE) activity and NP matrix biosynthesis. Static compression accelerates disc NP cell senescence compared with the dynamic compression in a disc organ culture. The present study provides that acceleration of NP cell senescence may be involved in previously reported static compression-mediated disc NP degenerative changes. Portland Press Ltd. 2018-03-09 /pmc/articles/PMC5843747/ /pubmed/29437905 http://dx.doi.org/10.1042/BSR20180064 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Shi, Jianmin Pang, Lianglong Jiao, Shouguo The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture |
title | The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture |
title_full | The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture |
title_fullStr | The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture |
title_full_unstemmed | The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture |
title_short | The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture |
title_sort | response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843747/ https://www.ncbi.nlm.nih.gov/pubmed/29437905 http://dx.doi.org/10.1042/BSR20180064 |
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