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Quantification of systemic renin-angiotensin system peptides of hypertensive black and white African men established from the RAS-Fingerprint®
OBJECTIVE: The objective of this study was to make use of a quantitative and qualitative approach comparing the systemic renin-angiotensin system (RAS) of hypertensive black and white African men by using RAS equilibrium analysis. MATERIALS AND METHODS: This sub-study involved 23 black (n = 15) and...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843883/ https://www.ncbi.nlm.nih.gov/pubmed/27737932 http://dx.doi.org/10.1177/1470320316669880 |
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author | van Rooyen, JM Poglitsch, M Huisman, HW Mels, CMC Kruger, R Malan, L Botha, S Lammertyn, L Gafane, L Schutte, AE |
author_facet | van Rooyen, JM Poglitsch, M Huisman, HW Mels, CMC Kruger, R Malan, L Botha, S Lammertyn, L Gafane, L Schutte, AE |
author_sort | van Rooyen, JM |
collection | PubMed |
description | OBJECTIVE: The objective of this study was to make use of a quantitative and qualitative approach comparing the systemic renin-angiotensin system (RAS) of hypertensive black and white African men by using RAS equilibrium analysis. MATERIALS AND METHODS: This sub-study involved 23 black (n = 15) and white (n = 8) hypertensive men aged 39.5–41 years, living in the North West Province of South Africa. The RAS-Fingerprinting was determined with LC-MS/MS quantification of angiotensin peptides. Blood pressure and other variables were determined with known methods. RESULTS: The main finding of this study was the significant lower Ang I (<5.0 and 45.1 pg/ml; p = 0.005) and Ang II (15.6 and 123.9 pg/ml; p ⩽ 0.001) encountered in the hypertensive black African men compared to their white counterparts. Levels of Ang 1-5 (downstream metabolite of Ang 1-7) (1.8 and 3.0 pg/ml), were detected in black and white hypertensive men, respectively. CONCLUSIONS: The observed differences between circulating RAS components, which are reflected via equilibrium angiotensin levels, point to a distinctive molecular regulation of the RAAS in the two study cohorts. The increased peripheral resistance observed in hypertensive black individuals might take over a dominant role in control of blood pressure in this study population. A novel highly sensitive LC-MS/MS method resolved the issue of peptide recovery variations during sample preparation by using internal standards for each individual angiotensin metabolite. |
format | Online Article Text |
id | pubmed-5843883 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-58438832018-03-20 Quantification of systemic renin-angiotensin system peptides of hypertensive black and white African men established from the RAS-Fingerprint® van Rooyen, JM Poglitsch, M Huisman, HW Mels, CMC Kruger, R Malan, L Botha, S Lammertyn, L Gafane, L Schutte, AE J Renin Angiotensin Aldosterone Syst Original Article OBJECTIVE: The objective of this study was to make use of a quantitative and qualitative approach comparing the systemic renin-angiotensin system (RAS) of hypertensive black and white African men by using RAS equilibrium analysis. MATERIALS AND METHODS: This sub-study involved 23 black (n = 15) and white (n = 8) hypertensive men aged 39.5–41 years, living in the North West Province of South Africa. The RAS-Fingerprinting was determined with LC-MS/MS quantification of angiotensin peptides. Blood pressure and other variables were determined with known methods. RESULTS: The main finding of this study was the significant lower Ang I (<5.0 and 45.1 pg/ml; p = 0.005) and Ang II (15.6 and 123.9 pg/ml; p ⩽ 0.001) encountered in the hypertensive black African men compared to their white counterparts. Levels of Ang 1-5 (downstream metabolite of Ang 1-7) (1.8 and 3.0 pg/ml), were detected in black and white hypertensive men, respectively. CONCLUSIONS: The observed differences between circulating RAS components, which are reflected via equilibrium angiotensin levels, point to a distinctive molecular regulation of the RAAS in the two study cohorts. The increased peripheral resistance observed in hypertensive black individuals might take over a dominant role in control of blood pressure in this study population. A novel highly sensitive LC-MS/MS method resolved the issue of peptide recovery variations during sample preparation by using internal standards for each individual angiotensin metabolite. SAGE Publications 2016-10-12 /pmc/articles/PMC5843883/ /pubmed/27737932 http://dx.doi.org/10.1177/1470320316669880 Text en © The Author(s) 2016 http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article van Rooyen, JM Poglitsch, M Huisman, HW Mels, CMC Kruger, R Malan, L Botha, S Lammertyn, L Gafane, L Schutte, AE Quantification of systemic renin-angiotensin system peptides of hypertensive black and white African men established from the RAS-Fingerprint® |
title | Quantification of systemic renin-angiotensin system peptides of hypertensive black and white African men established from the RAS-Fingerprint® |
title_full | Quantification of systemic renin-angiotensin system peptides of hypertensive black and white African men established from the RAS-Fingerprint® |
title_fullStr | Quantification of systemic renin-angiotensin system peptides of hypertensive black and white African men established from the RAS-Fingerprint® |
title_full_unstemmed | Quantification of systemic renin-angiotensin system peptides of hypertensive black and white African men established from the RAS-Fingerprint® |
title_short | Quantification of systemic renin-angiotensin system peptides of hypertensive black and white African men established from the RAS-Fingerprint® |
title_sort | quantification of systemic renin-angiotensin system peptides of hypertensive black and white african men established from the ras-fingerprint® |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843883/ https://www.ncbi.nlm.nih.gov/pubmed/27737932 http://dx.doi.org/10.1177/1470320316669880 |
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