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Extracellular vesicles as a platform for membrane-associated therapeutic protein delivery
Membrane proteins are of great research interest, particularly because they are rich in targets for therapeutic application. The suitability of various membrane proteins as targets for therapeutic formulations, such as drugs or antibodies, has been studied in preclinical and clinical studies. For th...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5844050/ https://www.ncbi.nlm.nih.gov/pubmed/29535849 http://dx.doi.org/10.1080/20013078.2018.1440131 |
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author | Yang, Yoosoo Hong, Yeonsun Cho, Eunji Kim, Gi Beom Kim, In-San |
author_facet | Yang, Yoosoo Hong, Yeonsun Cho, Eunji Kim, Gi Beom Kim, In-San |
author_sort | Yang, Yoosoo |
collection | PubMed |
description | Membrane proteins are of great research interest, particularly because they are rich in targets for therapeutic application. The suitability of various membrane proteins as targets for therapeutic formulations, such as drugs or antibodies, has been studied in preclinical and clinical studies. For therapeutic application, however, a protein must be expressed and purified in as close to its native conformation as possible. This has proven difficult for membrane proteins, as their native conformation requires the association with an appropriate cellular membrane. One solution to this problem is to use extracellular vesicles as a display platform. Exosomes and microvesicles are membranous extracellular vesicles that are released from most cells. Their membranes may provide a favourable microenvironment for membrane proteins to take on their proper conformation, activity, and membrane distribution; moreover, membrane proteins can cluster into microdomains on the surface of extracellular vesicles following their biogenesis. In this review, we survey the state-of-the-art of extracellular vesicle (exosome and small-sized microvesicle)-based therapeutics, evaluate the current biological understanding of these formulations, and forecast the technical advances that will be needed to continue driving the development of membrane protein therapeutics. |
format | Online Article Text |
id | pubmed-5844050 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-58440502018-03-13 Extracellular vesicles as a platform for membrane-associated therapeutic protein delivery Yang, Yoosoo Hong, Yeonsun Cho, Eunji Kim, Gi Beom Kim, In-San J Extracell Vesicles Review Article Membrane proteins are of great research interest, particularly because they are rich in targets for therapeutic application. The suitability of various membrane proteins as targets for therapeutic formulations, such as drugs or antibodies, has been studied in preclinical and clinical studies. For therapeutic application, however, a protein must be expressed and purified in as close to its native conformation as possible. This has proven difficult for membrane proteins, as their native conformation requires the association with an appropriate cellular membrane. One solution to this problem is to use extracellular vesicles as a display platform. Exosomes and microvesicles are membranous extracellular vesicles that are released from most cells. Their membranes may provide a favourable microenvironment for membrane proteins to take on their proper conformation, activity, and membrane distribution; moreover, membrane proteins can cluster into microdomains on the surface of extracellular vesicles following their biogenesis. In this review, we survey the state-of-the-art of extracellular vesicle (exosome and small-sized microvesicle)-based therapeutics, evaluate the current biological understanding of these formulations, and forecast the technical advances that will be needed to continue driving the development of membrane protein therapeutics. Taylor & Francis 2018-03-01 /pmc/articles/PMC5844050/ /pubmed/29535849 http://dx.doi.org/10.1080/20013078.2018.1440131 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Article Yang, Yoosoo Hong, Yeonsun Cho, Eunji Kim, Gi Beom Kim, In-San Extracellular vesicles as a platform for membrane-associated therapeutic protein delivery |
title | Extracellular vesicles as a platform for membrane-associated therapeutic protein delivery |
title_full | Extracellular vesicles as a platform for membrane-associated therapeutic protein delivery |
title_fullStr | Extracellular vesicles as a platform for membrane-associated therapeutic protein delivery |
title_full_unstemmed | Extracellular vesicles as a platform for membrane-associated therapeutic protein delivery |
title_short | Extracellular vesicles as a platform for membrane-associated therapeutic protein delivery |
title_sort | extracellular vesicles as a platform for membrane-associated therapeutic protein delivery |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5844050/ https://www.ncbi.nlm.nih.gov/pubmed/29535849 http://dx.doi.org/10.1080/20013078.2018.1440131 |
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