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Construction of Designer Selectable Marker Deletions with a CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and New Design of Common Entry Vectors

Vectors encoding selectable markers have been widely used in yeast to maintain or express exogenous DNA fragments. In the fission yeast Schizosaccharomyces pombe, several engineered markers have been reported and widely used, such as ura4(+) and ScLEU2 from Saccharomyces cerevisiae, which complement...

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Detalles Bibliográficos
Autores principales:	Zhao, Yu, Boeke, Jef D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Genetics Society of America 2018
Materias:	Investigations
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5844300/ https://www.ncbi.nlm.nih.gov/pubmed/29321167 http://dx.doi.org/10.1534/g3.117.300363

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