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Identification and Validation of a New Source of Low Grain Cadmium Accumulation in Durum Wheat

Cadmium (Cd) is a heavy metal that has no known biological function and is toxic for many living organisms. The maximum level of Cd concentration allowed in the international market for wheat grain is 0.2 mg kg(−1). Because phenotyping for Cd uptake is expensive and time consuming, molecular markers...

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Autores principales: Oladzad-Abbasabadi, Atena, Kumar, Ajay, Pirseyedi, Seyed, Salsman, Evan, Dobrydina, Marina, Poudel, Roshan Sharma, AbuHammad, Wesam A., Chao, Shiaoman, Faris, Justin D., Elias, Elias M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5844312/
https://www.ncbi.nlm.nih.gov/pubmed/29352079
http://dx.doi.org/10.1534/g3.117.300370
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author Oladzad-Abbasabadi, Atena
Kumar, Ajay
Pirseyedi, Seyed
Salsman, Evan
Dobrydina, Marina
Poudel, Roshan Sharma
AbuHammad, Wesam A.
Chao, Shiaoman
Faris, Justin D.
Elias, Elias M.
author_facet Oladzad-Abbasabadi, Atena
Kumar, Ajay
Pirseyedi, Seyed
Salsman, Evan
Dobrydina, Marina
Poudel, Roshan Sharma
AbuHammad, Wesam A.
Chao, Shiaoman
Faris, Justin D.
Elias, Elias M.
author_sort Oladzad-Abbasabadi, Atena
collection PubMed
description Cadmium (Cd) is a heavy metal that has no known biological function and is toxic for many living organisms. The maximum level of Cd concentration allowed in the international market for wheat grain is 0.2 mg kg(−1). Because phenotyping for Cd uptake is expensive and time consuming, molecular markers associated with genes conferring low Cd uptake would expedite selection and lead to the development of durum cultivars with reduced Cd concentrations. Here, we identified single nucleotide polymorphisms (SNPs) associated with a novel low Cd uptake locus in the durum experimental line D041735, which has hexaploid common wheat in its pedigree. Genetic analysis revealed a single major QTL for Cd uptake on chromosome arm 5BL within a 0.3 cM interval flanked by SNP markers. Analysis of the intervening sequence revealed a gene with homology to an aluminum-induced protein as a candidate gene. Validation and allelism tests revealed that the low Cd uptake gene identified in this study is different from the closely linked Cdu1-B gene, which also resides on 5BL. This study therefore showed that the durum experimental line D041735 contains a novel low Cd uptake gene that was likely acquired from hexaploid wheat.
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spelling pubmed-58443122018-03-22 Identification and Validation of a New Source of Low Grain Cadmium Accumulation in Durum Wheat Oladzad-Abbasabadi, Atena Kumar, Ajay Pirseyedi, Seyed Salsman, Evan Dobrydina, Marina Poudel, Roshan Sharma AbuHammad, Wesam A. Chao, Shiaoman Faris, Justin D. Elias, Elias M. G3 (Bethesda) Investigations Cadmium (Cd) is a heavy metal that has no known biological function and is toxic for many living organisms. The maximum level of Cd concentration allowed in the international market for wheat grain is 0.2 mg kg(−1). Because phenotyping for Cd uptake is expensive and time consuming, molecular markers associated with genes conferring low Cd uptake would expedite selection and lead to the development of durum cultivars with reduced Cd concentrations. Here, we identified single nucleotide polymorphisms (SNPs) associated with a novel low Cd uptake locus in the durum experimental line D041735, which has hexaploid common wheat in its pedigree. Genetic analysis revealed a single major QTL for Cd uptake on chromosome arm 5BL within a 0.3 cM interval flanked by SNP markers. Analysis of the intervening sequence revealed a gene with homology to an aluminum-induced protein as a candidate gene. Validation and allelism tests revealed that the low Cd uptake gene identified in this study is different from the closely linked Cdu1-B gene, which also resides on 5BL. This study therefore showed that the durum experimental line D041735 contains a novel low Cd uptake gene that was likely acquired from hexaploid wheat. Genetics Society of America 2018-01-19 /pmc/articles/PMC5844312/ /pubmed/29352079 http://dx.doi.org/10.1534/g3.117.300370 Text en Copyright © 2018 Abbasabadi et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Oladzad-Abbasabadi, Atena
Kumar, Ajay
Pirseyedi, Seyed
Salsman, Evan
Dobrydina, Marina
Poudel, Roshan Sharma
AbuHammad, Wesam A.
Chao, Shiaoman
Faris, Justin D.
Elias, Elias M.
Identification and Validation of a New Source of Low Grain Cadmium Accumulation in Durum Wheat
title Identification and Validation of a New Source of Low Grain Cadmium Accumulation in Durum Wheat
title_full Identification and Validation of a New Source of Low Grain Cadmium Accumulation in Durum Wheat
title_fullStr Identification and Validation of a New Source of Low Grain Cadmium Accumulation in Durum Wheat
title_full_unstemmed Identification and Validation of a New Source of Low Grain Cadmium Accumulation in Durum Wheat
title_short Identification and Validation of a New Source of Low Grain Cadmium Accumulation in Durum Wheat
title_sort identification and validation of a new source of low grain cadmium accumulation in durum wheat
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5844312/
https://www.ncbi.nlm.nih.gov/pubmed/29352079
http://dx.doi.org/10.1534/g3.117.300370
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