Embryo development until blastocyst stage with and without renewal of single medium on day 3

OBJECTIVE: This study aimed to assess the effects of using a single culture medium not renewed on day 3 on the development of the embryo up to the blastocyst stage. METHODS: The study was carried out in the Assisted Reproduction Laboratory of Clínica Miraflores in the period ranging from April to De...

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Detalles Bibliográficos
Autores principales: Peña, Fernando, Dávalos, Rocío, Rechkemmer, Adolfo, Ascenzo, Alvaro, Gonzales, Mauricio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Brazilian Society of Assisted Reproduction 2018
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5844659/
https://www.ncbi.nlm.nih.gov/pubmed/29318868
http://dx.doi.org/10.5935/1518-0557.20180012
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author Peña, Fernando
Dávalos, Rocío
Rechkemmer, Adolfo
Ascenzo, Alvaro
Gonzales, Mauricio
author_facet Peña, Fernando
Dávalos, Rocío
Rechkemmer, Adolfo
Ascenzo, Alvaro
Gonzales, Mauricio
author_sort Peña, Fernando
collection PubMed
description OBJECTIVE: This study aimed to assess the effects of using a single culture medium not renewed on day 3 on the development of the embryo up to the blastocyst stage. METHODS: The study was carried out in the Assisted Reproduction Laboratory of Clínica Miraflores in the period ranging from April to December of 2016. The study included 589 human embryos obtained from 82 couples submitted to IVF/ICSI with donor oocytes. The couples were randomly divided into two groups: group 1 (medium renewed on day 3) and group 2 (medium not renewed on day 3). RESULTS: Significant differences in pregnancy rates, implantation rates, and blastocyst formation were not observed. CONCLUSION: No statistically significant difference was found between the groups with respect to the analyzed parameters. However, a non-significant trend was observed in favor of the group without medium renewal for clinical pregnancy and positive pregnancy rates (β-hCG).
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spelling pubmed-58446592018-03-22 Embryo development until blastocyst stage with and without renewal of single medium on day 3 Peña, Fernando Dávalos, Rocío Rechkemmer, Adolfo Ascenzo, Alvaro Gonzales, Mauricio JBRA Assist Reprod Original Article OBJECTIVE: This study aimed to assess the effects of using a single culture medium not renewed on day 3 on the development of the embryo up to the blastocyst stage. METHODS: The study was carried out in the Assisted Reproduction Laboratory of Clínica Miraflores in the period ranging from April to December of 2016. The study included 589 human embryos obtained from 82 couples submitted to IVF/ICSI with donor oocytes. The couples were randomly divided into two groups: group 1 (medium renewed on day 3) and group 2 (medium not renewed on day 3). RESULTS: Significant differences in pregnancy rates, implantation rates, and blastocyst formation were not observed. CONCLUSION: No statistically significant difference was found between the groups with respect to the analyzed parameters. However, a non-significant trend was observed in favor of the group without medium renewal for clinical pregnancy and positive pregnancy rates (β-hCG). Brazilian Society of Assisted Reproduction 2018 /pmc/articles/PMC5844659/ /pubmed/29318868 http://dx.doi.org/10.5935/1518-0557.20180012 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Peña, Fernando
Dávalos, Rocío
Rechkemmer, Adolfo
Ascenzo, Alvaro
Gonzales, Mauricio
Embryo development until blastocyst stage with and without renewal of single medium on day 3
title Embryo development until blastocyst stage with and without renewal of single medium on day 3
title_full Embryo development until blastocyst stage with and without renewal of single medium on day 3
title_fullStr Embryo development until blastocyst stage with and without renewal of single medium on day 3
title_full_unstemmed Embryo development until blastocyst stage with and without renewal of single medium on day 3
title_short Embryo development until blastocyst stage with and without renewal of single medium on day 3
title_sort embryo development until blastocyst stage with and without renewal of single medium on day 3
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5844659/
https://www.ncbi.nlm.nih.gov/pubmed/29318868
http://dx.doi.org/10.5935/1518-0557.20180012
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