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Transcriptome and Cell Physiological Analyses in Different Rice Cultivars Provide New Insights Into Adaptive and Salinity Stress Responses
Salinity tolerance has been extensively investigated in recent years due to its agricultural importance. Several features, such as the regulation of ionic transporters and metabolic adjustments, have been identified as salt tolerance hallmarks. Nevertheless, due to the complexity of the trait, the r...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5844958/ https://www.ncbi.nlm.nih.gov/pubmed/29556243 http://dx.doi.org/10.3389/fpls.2018.00204 |
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author | Formentin, Elide Sudiro, Cristina Perin, Giorgio Riccadonna, Samantha Barizza, Elisabetta Baldoni, Elena Lavezzo, Enrico Stevanato, Piergiorgio Sacchi, Gian Attilio Fontana, Paolo Toppo, Stefano Morosinotto, Tomas Zottini, Michela Lo Schiavo, Fiorella |
author_facet | Formentin, Elide Sudiro, Cristina Perin, Giorgio Riccadonna, Samantha Barizza, Elisabetta Baldoni, Elena Lavezzo, Enrico Stevanato, Piergiorgio Sacchi, Gian Attilio Fontana, Paolo Toppo, Stefano Morosinotto, Tomas Zottini, Michela Lo Schiavo, Fiorella |
author_sort | Formentin, Elide |
collection | PubMed |
description | Salinity tolerance has been extensively investigated in recent years due to its agricultural importance. Several features, such as the regulation of ionic transporters and metabolic adjustments, have been identified as salt tolerance hallmarks. Nevertheless, due to the complexity of the trait, the results achieved to date have met with limited success in improving the salt tolerance of rice plants when tested in the field, thus suggesting that a better understanding of the tolerance mechanisms is still required. In this work, differences between two varieties of rice with contrasting salt sensitivities were revealed by the imaging of photosynthetic parameters, ion content analysis and a transcriptomic approach. The transcriptomic analysis conducted on tolerant plants supported the setting up of an adaptive program consisting of sodium distribution preferentially limited to the roots and older leaves, and in the activation of regulatory mechanisms of photosynthesis in the new leaves. As a result, plants resumed grow even under prolonged saline stress. In contrast, in the sensitive variety, RNA-seq analysis revealed a misleading response, ending in senescence and cell death. The physiological response at the cellular level was investigated by measuring the intracellular profile of H(2)O(2) in the roots, using a fluorescent probe. In the roots of tolerant plants, a quick response was observed with an increase in H(2)O(2) production within 5 min after salt treatment. The expression analysis of some of the genes involved in perception, signal transduction and salt stress response confirmed their early induction in the roots of tolerant plants compared to sensitive ones. By inhibiting the synthesis of apoplastic H(2)O(2), a reduction in the expression of these genes was detected. Our results indicate that quick H(2)O(2) signaling in the roots is part of a coordinated response that leads to adaptation instead of senescence in salt-treated rice plants. |
format | Online Article Text |
id | pubmed-5844958 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58449582018-03-19 Transcriptome and Cell Physiological Analyses in Different Rice Cultivars Provide New Insights Into Adaptive and Salinity Stress Responses Formentin, Elide Sudiro, Cristina Perin, Giorgio Riccadonna, Samantha Barizza, Elisabetta Baldoni, Elena Lavezzo, Enrico Stevanato, Piergiorgio Sacchi, Gian Attilio Fontana, Paolo Toppo, Stefano Morosinotto, Tomas Zottini, Michela Lo Schiavo, Fiorella Front Plant Sci Plant Science Salinity tolerance has been extensively investigated in recent years due to its agricultural importance. Several features, such as the regulation of ionic transporters and metabolic adjustments, have been identified as salt tolerance hallmarks. Nevertheless, due to the complexity of the trait, the results achieved to date have met with limited success in improving the salt tolerance of rice plants when tested in the field, thus suggesting that a better understanding of the tolerance mechanisms is still required. In this work, differences between two varieties of rice with contrasting salt sensitivities were revealed by the imaging of photosynthetic parameters, ion content analysis and a transcriptomic approach. The transcriptomic analysis conducted on tolerant plants supported the setting up of an adaptive program consisting of sodium distribution preferentially limited to the roots and older leaves, and in the activation of regulatory mechanisms of photosynthesis in the new leaves. As a result, plants resumed grow even under prolonged saline stress. In contrast, in the sensitive variety, RNA-seq analysis revealed a misleading response, ending in senescence and cell death. The physiological response at the cellular level was investigated by measuring the intracellular profile of H(2)O(2) in the roots, using a fluorescent probe. In the roots of tolerant plants, a quick response was observed with an increase in H(2)O(2) production within 5 min after salt treatment. The expression analysis of some of the genes involved in perception, signal transduction and salt stress response confirmed their early induction in the roots of tolerant plants compared to sensitive ones. By inhibiting the synthesis of apoplastic H(2)O(2), a reduction in the expression of these genes was detected. Our results indicate that quick H(2)O(2) signaling in the roots is part of a coordinated response that leads to adaptation instead of senescence in salt-treated rice plants. Frontiers Media S.A. 2018-03-05 /pmc/articles/PMC5844958/ /pubmed/29556243 http://dx.doi.org/10.3389/fpls.2018.00204 Text en Copyright © 2018 Formentin, Sudiro, Perin, Riccadonna, Barizza, Baldoni, Lavezzo, Stevanato, Sacchi, Fontana, Toppo, Morosinotto, Zottini and Lo Schiavo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Formentin, Elide Sudiro, Cristina Perin, Giorgio Riccadonna, Samantha Barizza, Elisabetta Baldoni, Elena Lavezzo, Enrico Stevanato, Piergiorgio Sacchi, Gian Attilio Fontana, Paolo Toppo, Stefano Morosinotto, Tomas Zottini, Michela Lo Schiavo, Fiorella Transcriptome and Cell Physiological Analyses in Different Rice Cultivars Provide New Insights Into Adaptive and Salinity Stress Responses |
title | Transcriptome and Cell Physiological Analyses in Different Rice Cultivars Provide New Insights Into Adaptive and Salinity Stress Responses |
title_full | Transcriptome and Cell Physiological Analyses in Different Rice Cultivars Provide New Insights Into Adaptive and Salinity Stress Responses |
title_fullStr | Transcriptome and Cell Physiological Analyses in Different Rice Cultivars Provide New Insights Into Adaptive and Salinity Stress Responses |
title_full_unstemmed | Transcriptome and Cell Physiological Analyses in Different Rice Cultivars Provide New Insights Into Adaptive and Salinity Stress Responses |
title_short | Transcriptome and Cell Physiological Analyses in Different Rice Cultivars Provide New Insights Into Adaptive and Salinity Stress Responses |
title_sort | transcriptome and cell physiological analyses in different rice cultivars provide new insights into adaptive and salinity stress responses |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5844958/ https://www.ncbi.nlm.nih.gov/pubmed/29556243 http://dx.doi.org/10.3389/fpls.2018.00204 |
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