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Ultraviolet-irradiated endothelial cells secrete stem cell factor and induce epidermal pigmentation
Ultraviolet (UV)-associated hyperpigmented skins are characterized with increased vasculature underlying pigmentation, suggestive of the possible biological role of endothelial cells in the regulation of skin pigmentation during UV irradiation. In this study, we showed that UV-irradiated endothelial...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5844989/ https://www.ncbi.nlm.nih.gov/pubmed/29523807 http://dx.doi.org/10.1038/s41598-018-22608-y |
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author | Kim, Misun Shibata, Takako Kwon, Soohyun Park, Tae Jun Kang, Hee Young |
author_facet | Kim, Misun Shibata, Takako Kwon, Soohyun Park, Tae Jun Kang, Hee Young |
author_sort | Kim, Misun |
collection | PubMed |
description | Ultraviolet (UV)-associated hyperpigmented skins are characterized with increased vasculature underlying pigmentation, suggestive of the possible biological role of endothelial cells in the regulation of skin pigmentation during UV irradiation. In this study, we showed that UV-irradiated endothelial cells significantly increased the pigmentation of melanocytes through epithelial-mesenchymal crosstalk. The stimulatory effect of endothelial cells was further demonstrated using ex vivo human skin. RNA sequence analysis and enzyme-linked immunosorbent assay showed that endothelial cells secrete more stem cell factor (SCF) upon UV irradiation than non-irradiated cells. The increased pigmentation elicited by endothelial cells was abrogated following inhibition of SCF/c-KIT signaling. Together these results suggest that endothelial cells are activated upon UV exposure to release melanogenic factors such as SCF, which contributes to the development of skin hyperpigmentation during chronic sun exposure. |
format | Online Article Text |
id | pubmed-5844989 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58449892018-03-14 Ultraviolet-irradiated endothelial cells secrete stem cell factor and induce epidermal pigmentation Kim, Misun Shibata, Takako Kwon, Soohyun Park, Tae Jun Kang, Hee Young Sci Rep Article Ultraviolet (UV)-associated hyperpigmented skins are characterized with increased vasculature underlying pigmentation, suggestive of the possible biological role of endothelial cells in the regulation of skin pigmentation during UV irradiation. In this study, we showed that UV-irradiated endothelial cells significantly increased the pigmentation of melanocytes through epithelial-mesenchymal crosstalk. The stimulatory effect of endothelial cells was further demonstrated using ex vivo human skin. RNA sequence analysis and enzyme-linked immunosorbent assay showed that endothelial cells secrete more stem cell factor (SCF) upon UV irradiation than non-irradiated cells. The increased pigmentation elicited by endothelial cells was abrogated following inhibition of SCF/c-KIT signaling. Together these results suggest that endothelial cells are activated upon UV exposure to release melanogenic factors such as SCF, which contributes to the development of skin hyperpigmentation during chronic sun exposure. Nature Publishing Group UK 2018-03-09 /pmc/articles/PMC5844989/ /pubmed/29523807 http://dx.doi.org/10.1038/s41598-018-22608-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kim, Misun Shibata, Takako Kwon, Soohyun Park, Tae Jun Kang, Hee Young Ultraviolet-irradiated endothelial cells secrete stem cell factor and induce epidermal pigmentation |
title | Ultraviolet-irradiated endothelial cells secrete stem cell factor and induce epidermal pigmentation |
title_full | Ultraviolet-irradiated endothelial cells secrete stem cell factor and induce epidermal pigmentation |
title_fullStr | Ultraviolet-irradiated endothelial cells secrete stem cell factor and induce epidermal pigmentation |
title_full_unstemmed | Ultraviolet-irradiated endothelial cells secrete stem cell factor and induce epidermal pigmentation |
title_short | Ultraviolet-irradiated endothelial cells secrete stem cell factor and induce epidermal pigmentation |
title_sort | ultraviolet-irradiated endothelial cells secrete stem cell factor and induce epidermal pigmentation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5844989/ https://www.ncbi.nlm.nih.gov/pubmed/29523807 http://dx.doi.org/10.1038/s41598-018-22608-y |
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