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A novel monoclonal antibody efficiently blocks the infection of serotype 4 fowl adenovirus by targeting fiber-2

A recent outbreak of hepatitis–hydropericardium syndrome caused by serotype 4 fowl adenovirus (FAdV-4) has resulted in significant economic losses to the poultry industry worldwide. However, little is known about the molecular pathogenesis of FAdV-4. In this study, a novel monoclonal antibody (mAb)...

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Autores principales: Wang, Ping, Zhang, Jianjun, Wang, Weikang, Li, Tuofan, Liang, Guangchen, Shao, Hongxia, Gao, Wei, Qin, Aijian, Ye, Jianqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5845368/
https://www.ncbi.nlm.nih.gov/pubmed/29523195
http://dx.doi.org/10.1186/s13567-018-0525-y
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author Wang, Ping
Zhang, Jianjun
Wang, Weikang
Li, Tuofan
Liang, Guangchen
Shao, Hongxia
Gao, Wei
Qin, Aijian
Ye, Jianqiang
author_facet Wang, Ping
Zhang, Jianjun
Wang, Weikang
Li, Tuofan
Liang, Guangchen
Shao, Hongxia
Gao, Wei
Qin, Aijian
Ye, Jianqiang
author_sort Wang, Ping
collection PubMed
description A recent outbreak of hepatitis–hydropericardium syndrome caused by serotype 4 fowl adenovirus (FAdV-4) has resulted in significant economic losses to the poultry industry worldwide. However, little is known about the molecular pathogenesis of FAdV-4. In this study, a novel monoclonal antibody (mAb) targeting the fiber-2 protein of FAdV-4 was generated, mAb 3C2. Indirect immunofluorescence assay showed that mAb 3C2 neither reacted with serotype 8 fowl adenovirus (FAdV-8) nor reacted with the fiber-1 protein of FAdV-4; it specifically reacted with the fiber-2 protein of FAdV-4. Notably, mAb 3C2 could efficiently immunoprecipitate the fiber-2 protein in chicken liver cells either infected with FAdV-4 or transfected with pcDNA3.1-Fiber2. Moreover, mAb 3C2 demonstrated marked neutralizing activity against FAdV-4 and could efficiently inhibit the infection of FAdV-4 in vitro. Using truncated fiber-2 constructs, the epitope recognized by mAb 3C2 was determined to be located between amino acids 416–448 at the C-terminus of fiber-2. Our data not only provide a foundation for the establishment of a rapid fiber-2 peptide-based diagnostic assay for FAdV-4 but also highlight the critical role of the fiber-2 protein in mediating infection by FAdV-4. Furthermore, the epitope recognized by 3C2 might serve as a novel target for the development of a vaccine targeting FAdV-4.
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spelling pubmed-58453682018-03-19 A novel monoclonal antibody efficiently blocks the infection of serotype 4 fowl adenovirus by targeting fiber-2 Wang, Ping Zhang, Jianjun Wang, Weikang Li, Tuofan Liang, Guangchen Shao, Hongxia Gao, Wei Qin, Aijian Ye, Jianqiang Vet Res Research Article A recent outbreak of hepatitis–hydropericardium syndrome caused by serotype 4 fowl adenovirus (FAdV-4) has resulted in significant economic losses to the poultry industry worldwide. However, little is known about the molecular pathogenesis of FAdV-4. In this study, a novel monoclonal antibody (mAb) targeting the fiber-2 protein of FAdV-4 was generated, mAb 3C2. Indirect immunofluorescence assay showed that mAb 3C2 neither reacted with serotype 8 fowl adenovirus (FAdV-8) nor reacted with the fiber-1 protein of FAdV-4; it specifically reacted with the fiber-2 protein of FAdV-4. Notably, mAb 3C2 could efficiently immunoprecipitate the fiber-2 protein in chicken liver cells either infected with FAdV-4 or transfected with pcDNA3.1-Fiber2. Moreover, mAb 3C2 demonstrated marked neutralizing activity against FAdV-4 and could efficiently inhibit the infection of FAdV-4 in vitro. Using truncated fiber-2 constructs, the epitope recognized by mAb 3C2 was determined to be located between amino acids 416–448 at the C-terminus of fiber-2. Our data not only provide a foundation for the establishment of a rapid fiber-2 peptide-based diagnostic assay for FAdV-4 but also highlight the critical role of the fiber-2 protein in mediating infection by FAdV-4. Furthermore, the epitope recognized by 3C2 might serve as a novel target for the development of a vaccine targeting FAdV-4. BioMed Central 2018-03-09 2018 /pmc/articles/PMC5845368/ /pubmed/29523195 http://dx.doi.org/10.1186/s13567-018-0525-y Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Wang, Ping
Zhang, Jianjun
Wang, Weikang
Li, Tuofan
Liang, Guangchen
Shao, Hongxia
Gao, Wei
Qin, Aijian
Ye, Jianqiang
A novel monoclonal antibody efficiently blocks the infection of serotype 4 fowl adenovirus by targeting fiber-2
title A novel monoclonal antibody efficiently blocks the infection of serotype 4 fowl adenovirus by targeting fiber-2
title_full A novel monoclonal antibody efficiently blocks the infection of serotype 4 fowl adenovirus by targeting fiber-2
title_fullStr A novel monoclonal antibody efficiently blocks the infection of serotype 4 fowl adenovirus by targeting fiber-2
title_full_unstemmed A novel monoclonal antibody efficiently blocks the infection of serotype 4 fowl adenovirus by targeting fiber-2
title_short A novel monoclonal antibody efficiently blocks the infection of serotype 4 fowl adenovirus by targeting fiber-2
title_sort novel monoclonal antibody efficiently blocks the infection of serotype 4 fowl adenovirus by targeting fiber-2
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5845368/
https://www.ncbi.nlm.nih.gov/pubmed/29523195
http://dx.doi.org/10.1186/s13567-018-0525-y
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