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Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip

This study was designed to prepare and evaluate the sensitivity and specificity of a Norovirus GI and GII fluorescent particles combined detection test strip method. Using selected chromatographic materials and antibodies specific to Norovirus GI and GII, the Norovirus GI and GII fluorescent particl...

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Autores principales: Zhang, Lifang, Peng, Yanxia, Jiang, Na, Shi, Lei, Lin, Jieping, Wu, Ping, Pan, Qingjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5845527/
https://www.ncbi.nlm.nih.gov/pubmed/29682555
http://dx.doi.org/10.1155/2018/7862467
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author Zhang, Lifang
Peng, Yanxia
Jiang, Na
Shi, Lei
Lin, Jieping
Wu, Ping
Pan, Qingjun
author_facet Zhang, Lifang
Peng, Yanxia
Jiang, Na
Shi, Lei
Lin, Jieping
Wu, Ping
Pan, Qingjun
author_sort Zhang, Lifang
collection PubMed
description This study was designed to prepare and evaluate the sensitivity and specificity of a Norovirus GI and GII fluorescent particles combined detection test strip method. Using selected chromatographic materials and antibodies specific to Norovirus GI and GII, the Norovirus GI and GII fluorescent particles combined detection test strip (tested method) was prepared as a conventional double antibody sandwich. The samples assayed included cultured rotavirus and 465 specimens from patients with symptoms of gastrointestinal infection. Norovirus was detected using the tested method and a reference method (CerTest Norovirus GI-GII test card). The results indicated that the sensitivity of the tested method was 4 (for GI detection) or 8 times (for GII detection) greater than the reference method. Neither of the two methods cross-reacted with rotavirus and so on. For specimens, 29 were found to be negative by the reference method and positive by the tested method, and 8 were found to be negative by the tested method and positive by the reference method. Furthermore, a retesting of these samples by qPCR showed that 28 of the 29 were positive, and 3 of the 8 were positive. In summary, the Norovirus GI and GII fluorescent particles combined detection test strip was successfully prepared and had good detection performance.
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spelling pubmed-58455272018-04-21 Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip Zhang, Lifang Peng, Yanxia Jiang, Na Shi, Lei Lin, Jieping Wu, Ping Pan, Qingjun Biomed Res Int Research Article This study was designed to prepare and evaluate the sensitivity and specificity of a Norovirus GI and GII fluorescent particles combined detection test strip method. Using selected chromatographic materials and antibodies specific to Norovirus GI and GII, the Norovirus GI and GII fluorescent particles combined detection test strip (tested method) was prepared as a conventional double antibody sandwich. The samples assayed included cultured rotavirus and 465 specimens from patients with symptoms of gastrointestinal infection. Norovirus was detected using the tested method and a reference method (CerTest Norovirus GI-GII test card). The results indicated that the sensitivity of the tested method was 4 (for GI detection) or 8 times (for GII detection) greater than the reference method. Neither of the two methods cross-reacted with rotavirus and so on. For specimens, 29 were found to be negative by the reference method and positive by the tested method, and 8 were found to be negative by the tested method and positive by the reference method. Furthermore, a retesting of these samples by qPCR showed that 28 of the 29 were positive, and 3 of the 8 were positive. In summary, the Norovirus GI and GII fluorescent particles combined detection test strip was successfully prepared and had good detection performance. Hindawi 2018-02-25 /pmc/articles/PMC5845527/ /pubmed/29682555 http://dx.doi.org/10.1155/2018/7862467 Text en Copyright © 2018 Lifang Zhang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Lifang
Peng, Yanxia
Jiang, Na
Shi, Lei
Lin, Jieping
Wu, Ping
Pan, Qingjun
Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip
title Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip
title_full Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip
title_fullStr Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip
title_full_unstemmed Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip
title_short Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip
title_sort preparation and evaluation of combined detection of norovirus gi and gii: an innovative fluorescent particles test strip
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5845527/
https://www.ncbi.nlm.nih.gov/pubmed/29682555
http://dx.doi.org/10.1155/2018/7862467
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