Cargando…
Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip
This study was designed to prepare and evaluate the sensitivity and specificity of a Norovirus GI and GII fluorescent particles combined detection test strip method. Using selected chromatographic materials and antibodies specific to Norovirus GI and GII, the Norovirus GI and GII fluorescent particl...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5845527/ https://www.ncbi.nlm.nih.gov/pubmed/29682555 http://dx.doi.org/10.1155/2018/7862467 |
_version_ | 1783305444121378816 |
---|---|
author | Zhang, Lifang Peng, Yanxia Jiang, Na Shi, Lei Lin, Jieping Wu, Ping Pan, Qingjun |
author_facet | Zhang, Lifang Peng, Yanxia Jiang, Na Shi, Lei Lin, Jieping Wu, Ping Pan, Qingjun |
author_sort | Zhang, Lifang |
collection | PubMed |
description | This study was designed to prepare and evaluate the sensitivity and specificity of a Norovirus GI and GII fluorescent particles combined detection test strip method. Using selected chromatographic materials and antibodies specific to Norovirus GI and GII, the Norovirus GI and GII fluorescent particles combined detection test strip (tested method) was prepared as a conventional double antibody sandwich. The samples assayed included cultured rotavirus and 465 specimens from patients with symptoms of gastrointestinal infection. Norovirus was detected using the tested method and a reference method (CerTest Norovirus GI-GII test card). The results indicated that the sensitivity of the tested method was 4 (for GI detection) or 8 times (for GII detection) greater than the reference method. Neither of the two methods cross-reacted with rotavirus and so on. For specimens, 29 were found to be negative by the reference method and positive by the tested method, and 8 were found to be negative by the tested method and positive by the reference method. Furthermore, a retesting of these samples by qPCR showed that 28 of the 29 were positive, and 3 of the 8 were positive. In summary, the Norovirus GI and GII fluorescent particles combined detection test strip was successfully prepared and had good detection performance. |
format | Online Article Text |
id | pubmed-5845527 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-58455272018-04-21 Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip Zhang, Lifang Peng, Yanxia Jiang, Na Shi, Lei Lin, Jieping Wu, Ping Pan, Qingjun Biomed Res Int Research Article This study was designed to prepare and evaluate the sensitivity and specificity of a Norovirus GI and GII fluorescent particles combined detection test strip method. Using selected chromatographic materials and antibodies specific to Norovirus GI and GII, the Norovirus GI and GII fluorescent particles combined detection test strip (tested method) was prepared as a conventional double antibody sandwich. The samples assayed included cultured rotavirus and 465 specimens from patients with symptoms of gastrointestinal infection. Norovirus was detected using the tested method and a reference method (CerTest Norovirus GI-GII test card). The results indicated that the sensitivity of the tested method was 4 (for GI detection) or 8 times (for GII detection) greater than the reference method. Neither of the two methods cross-reacted with rotavirus and so on. For specimens, 29 were found to be negative by the reference method and positive by the tested method, and 8 were found to be negative by the tested method and positive by the reference method. Furthermore, a retesting of these samples by qPCR showed that 28 of the 29 were positive, and 3 of the 8 were positive. In summary, the Norovirus GI and GII fluorescent particles combined detection test strip was successfully prepared and had good detection performance. Hindawi 2018-02-25 /pmc/articles/PMC5845527/ /pubmed/29682555 http://dx.doi.org/10.1155/2018/7862467 Text en Copyright © 2018 Lifang Zhang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhang, Lifang Peng, Yanxia Jiang, Na Shi, Lei Lin, Jieping Wu, Ping Pan, Qingjun Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip |
title | Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip |
title_full | Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip |
title_fullStr | Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip |
title_full_unstemmed | Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip |
title_short | Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip |
title_sort | preparation and evaluation of combined detection of norovirus gi and gii: an innovative fluorescent particles test strip |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5845527/ https://www.ncbi.nlm.nih.gov/pubmed/29682555 http://dx.doi.org/10.1155/2018/7862467 |
work_keys_str_mv | AT zhanglifang preparationandevaluationofcombineddetectionofnorovirusgiandgiianinnovativefluorescentparticlesteststrip AT pengyanxia preparationandevaluationofcombineddetectionofnorovirusgiandgiianinnovativefluorescentparticlesteststrip AT jiangna preparationandevaluationofcombineddetectionofnorovirusgiandgiianinnovativefluorescentparticlesteststrip AT shilei preparationandevaluationofcombineddetectionofnorovirusgiandgiianinnovativefluorescentparticlesteststrip AT linjieping preparationandevaluationofcombineddetectionofnorovirusgiandgiianinnovativefluorescentparticlesteststrip AT wuping preparationandevaluationofcombineddetectionofnorovirusgiandgiianinnovativefluorescentparticlesteststrip AT panqingjun preparationandevaluationofcombineddetectionofnorovirusgiandgiianinnovativefluorescentparticlesteststrip |