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High levels of macrolide-resistant Mycoplasma genitalium in Queensland, Australia

The macrolide azithromycin is recommended for treatment of Mycoplasma genitalium infection; however, M. genitalium strains possessing macrolide resistance-mediating mutations (MRMMs) are increasingly being reported. Here, we used the SpeeDx ResistancePlus MG kit, which provides simultaneous detectio...

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Autores principales: Trembizki, Ella, Buckley, Cameron, Bletchly, Cheryl, Nimmo, Graeme R., Whiley, David M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5845567/
https://www.ncbi.nlm.nih.gov/pubmed/28893363
http://dx.doi.org/10.1099/jmm.0.000584
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author Trembizki, Ella
Buckley, Cameron
Bletchly, Cheryl
Nimmo, Graeme R.
Whiley, David M.
author_facet Trembizki, Ella
Buckley, Cameron
Bletchly, Cheryl
Nimmo, Graeme R.
Whiley, David M.
author_sort Trembizki, Ella
collection PubMed
description The macrolide azithromycin is recommended for treatment of Mycoplasma genitalium infection; however, M. genitalium strains possessing macrolide resistance-mediating mutations (MRMMs) are increasingly being reported. Here, we used the SpeeDx ResistancePlus MG kit, which provides simultaneous detection of M. genitalium and MRMMs, to assess MRMM carriage among M. genitalium infections in Queensland, Australia. Performance characteristics of the ResistancePlus MG kit for M. genitalium detection were compared to in-house PCR. Available M. genitalium PCR-positive (n=67) and negative (n=281) samples from the years 2011 to 2017 were tested using the SpeeDx ResistancePlus MG kit. In total, 63.6 % M. genitalium-positive samples were indicated to harbour MRMMs. The ResistancePlus MG method provided sensitivity and specificity of 97 and 99.6 % respectively compared to in-house PCR for M. genitalium detection. Such high levels of macrolide-resistant M. genitalium raise further concerns over future use of azithromycin for treatment of M. genitalium infection.
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spelling pubmed-58455672018-03-13 High levels of macrolide-resistant Mycoplasma genitalium in Queensland, Australia Trembizki, Ella Buckley, Cameron Bletchly, Cheryl Nimmo, Graeme R. Whiley, David M. J Med Microbiol Short Communication The macrolide azithromycin is recommended for treatment of Mycoplasma genitalium infection; however, M. genitalium strains possessing macrolide resistance-mediating mutations (MRMMs) are increasingly being reported. Here, we used the SpeeDx ResistancePlus MG kit, which provides simultaneous detection of M. genitalium and MRMMs, to assess MRMM carriage among M. genitalium infections in Queensland, Australia. Performance characteristics of the ResistancePlus MG kit for M. genitalium detection were compared to in-house PCR. Available M. genitalium PCR-positive (n=67) and negative (n=281) samples from the years 2011 to 2017 were tested using the SpeeDx ResistancePlus MG kit. In total, 63.6 % M. genitalium-positive samples were indicated to harbour MRMMs. The ResistancePlus MG method provided sensitivity and specificity of 97 and 99.6 % respectively compared to in-house PCR for M. genitalium detection. Such high levels of macrolide-resistant M. genitalium raise further concerns over future use of azithromycin for treatment of M. genitalium infection. Microbiology Society 2017-10 2017-09-12 /pmc/articles/PMC5845567/ /pubmed/28893363 http://dx.doi.org/10.1099/jmm.0.000584 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
spellingShingle Short Communication
Trembizki, Ella
Buckley, Cameron
Bletchly, Cheryl
Nimmo, Graeme R.
Whiley, David M.
High levels of macrolide-resistant Mycoplasma genitalium in Queensland, Australia
title High levels of macrolide-resistant Mycoplasma genitalium in Queensland, Australia
title_full High levels of macrolide-resistant Mycoplasma genitalium in Queensland, Australia
title_fullStr High levels of macrolide-resistant Mycoplasma genitalium in Queensland, Australia
title_full_unstemmed High levels of macrolide-resistant Mycoplasma genitalium in Queensland, Australia
title_short High levels of macrolide-resistant Mycoplasma genitalium in Queensland, Australia
title_sort high levels of macrolide-resistant mycoplasma genitalium in queensland, australia
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5845567/
https://www.ncbi.nlm.nih.gov/pubmed/28893363
http://dx.doi.org/10.1099/jmm.0.000584
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