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The MtrAB two-component system controls antibiotic production in Streptomyces coelicolor A3(2)

MtrAB is a highly conserved two-component system implicated in the regulation of cell division in the Actinobacteria. It coordinates DNA replication with cell division in the unicellular Mycobacterium tuberculosis and links antibiotic production to sporulation in the filamentous Streptomyces venezue...

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Autores principales: Som, Nicolle F., Heine, Daniel, Holmes, Neil, Knowles, Felicity, Chandra, Govind, Seipke, Ryan F., Hoskisson, Paul A., Wilkinson, Barrie, Hutchings, Matthew I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5845573/
https://www.ncbi.nlm.nih.gov/pubmed/28884676
http://dx.doi.org/10.1099/mic.0.000524
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author Som, Nicolle F.
Heine, Daniel
Holmes, Neil
Knowles, Felicity
Chandra, Govind
Seipke, Ryan F.
Hoskisson, Paul A.
Wilkinson, Barrie
Hutchings, Matthew I.
author_facet Som, Nicolle F.
Heine, Daniel
Holmes, Neil
Knowles, Felicity
Chandra, Govind
Seipke, Ryan F.
Hoskisson, Paul A.
Wilkinson, Barrie
Hutchings, Matthew I.
author_sort Som, Nicolle F.
collection PubMed
description MtrAB is a highly conserved two-component system implicated in the regulation of cell division in the Actinobacteria. It coordinates DNA replication with cell division in the unicellular Mycobacterium tuberculosis and links antibiotic production to sporulation in the filamentous Streptomyces venezuelae. Chloramphenicol biosynthesis is directly regulated by MtrA in S. venezuelae and deletion of mtrB constitutively activates MtrA and results in constitutive over-production of chloramphenicol. Here we report that in Streptomyces coelicolor, MtrA binds to sites upstream of developmental genes and the genes encoding ActII-1, ActII-4 and RedZ, which are cluster-situated regulators of the antibiotics actinorhodin (Act) and undecylprodigiosin (Red). Consistent with this, deletion of mtrB switches on the production of Act, Red and streptorubin B, a product of the Red pathway. Thus, we propose that MtrA is a key regulator that links antibiotic production to development and can be used to upregulate antibiotic production in distantly related streptomycetes.
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spelling pubmed-58455732018-03-13 The MtrAB two-component system controls antibiotic production in Streptomyces coelicolor A3(2) Som, Nicolle F. Heine, Daniel Holmes, Neil Knowles, Felicity Chandra, Govind Seipke, Ryan F. Hoskisson, Paul A. Wilkinson, Barrie Hutchings, Matthew I. Microbiology (Reading) Short Communication MtrAB is a highly conserved two-component system implicated in the regulation of cell division in the Actinobacteria. It coordinates DNA replication with cell division in the unicellular Mycobacterium tuberculosis and links antibiotic production to sporulation in the filamentous Streptomyces venezuelae. Chloramphenicol biosynthesis is directly regulated by MtrA in S. venezuelae and deletion of mtrB constitutively activates MtrA and results in constitutive over-production of chloramphenicol. Here we report that in Streptomyces coelicolor, MtrA binds to sites upstream of developmental genes and the genes encoding ActII-1, ActII-4 and RedZ, which are cluster-situated regulators of the antibiotics actinorhodin (Act) and undecylprodigiosin (Red). Consistent with this, deletion of mtrB switches on the production of Act, Red and streptorubin B, a product of the Red pathway. Thus, we propose that MtrA is a key regulator that links antibiotic production to development and can be used to upregulate antibiotic production in distantly related streptomycetes. Microbiology Society 2017-10 2017-09-08 /pmc/articles/PMC5845573/ /pubmed/28884676 http://dx.doi.org/10.1099/mic.0.000524 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
spellingShingle Short Communication
Som, Nicolle F.
Heine, Daniel
Holmes, Neil
Knowles, Felicity
Chandra, Govind
Seipke, Ryan F.
Hoskisson, Paul A.
Wilkinson, Barrie
Hutchings, Matthew I.
The MtrAB two-component system controls antibiotic production in Streptomyces coelicolor A3(2)
title The MtrAB two-component system controls antibiotic production in Streptomyces coelicolor A3(2)
title_full The MtrAB two-component system controls antibiotic production in Streptomyces coelicolor A3(2)
title_fullStr The MtrAB two-component system controls antibiotic production in Streptomyces coelicolor A3(2)
title_full_unstemmed The MtrAB two-component system controls antibiotic production in Streptomyces coelicolor A3(2)
title_short The MtrAB two-component system controls antibiotic production in Streptomyces coelicolor A3(2)
title_sort mtrab two-component system controls antibiotic production in streptomyces coelicolor a3(2)
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5845573/
https://www.ncbi.nlm.nih.gov/pubmed/28884676
http://dx.doi.org/10.1099/mic.0.000524
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