Cargando…

Ferric Sulfate and Proline Enhance Heavy-Metal Tolerance of Halophilic/Halotolerant Soil Microorganisms and Their Bioremediation Potential for Spilled-Oil Under Multiple Stresses

The aim of this study was to explore the heavy-metal resistance and hydrocarbonoclastic potential of microorganisms in a hypersaline soil. For this, hydrocarbonoclastic microorganisms were counted on a mineral medium with oil vapor as a sole carbon source in the presence of increasing concentrations...

Descripción completa

Detalles Bibliográficos
Autores principales: Al-Mailem, Dina M., Eliyas, Mohamed, Radwan, Samir S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5845895/
https://www.ncbi.nlm.nih.gov/pubmed/29563904
http://dx.doi.org/10.3389/fmicb.2018.00394
_version_ 1783305502486167552
author Al-Mailem, Dina M.
Eliyas, Mohamed
Radwan, Samir S.
author_facet Al-Mailem, Dina M.
Eliyas, Mohamed
Radwan, Samir S.
author_sort Al-Mailem, Dina M.
collection PubMed
description The aim of this study was to explore the heavy-metal resistance and hydrocarbonoclastic potential of microorganisms in a hypersaline soil. For this, hydrocarbonoclastic microorganisms were counted on a mineral medium with oil vapor as a sole carbon source in the presence of increasing concentrations of ZnSO(4), HgCl(2), CdSO(4), PbNO(3), CuSO(4), and Na(2)HAsO(4.) The colony-forming units counted decreased in number from about 150 g(-1) on the heavy-metal-free medium to zero units on media with 40–100 mg l(-1) of HgCl(2), CdSO(4), PbNO(3), or Na(2)HAsO(4). On media with CuSO(4) or ZnSO(4) on the other hand, numbers increased first reaching maxima on media with 50 mg l(-1) CuSO(4) and 90 mg l(-1) ZnSO(4). Higher concentrations reduced the numbers, which however, still remained considerable. Pure microbial isolates in cultures tolerated 200–1600 mg l(-1) of HgCl(2), CdSO(4), PbNO(3), CuSO(4), and Na(2)HAsO(4) in the absence of crude oil. In the presence of oil vapor, the isolates tolerated much lower concentrations of the heavy metals, only 10–80 mg l(-1). The addition of 10 Fe(2)(SO(4))(3) and 200 mg l(-1) proline (by up to two- to threefold) enhanced the tolerance of several isolates to heavy metals, and consequently their potential for oil biodegradation in their presence. The results are useful in designing bioremediation technologies for oil spilled in hypersaline areas.
format Online
Article
Text
id pubmed-5845895
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-58458952018-03-21 Ferric Sulfate and Proline Enhance Heavy-Metal Tolerance of Halophilic/Halotolerant Soil Microorganisms and Their Bioremediation Potential for Spilled-Oil Under Multiple Stresses Al-Mailem, Dina M. Eliyas, Mohamed Radwan, Samir S. Front Microbiol Microbiology The aim of this study was to explore the heavy-metal resistance and hydrocarbonoclastic potential of microorganisms in a hypersaline soil. For this, hydrocarbonoclastic microorganisms were counted on a mineral medium with oil vapor as a sole carbon source in the presence of increasing concentrations of ZnSO(4), HgCl(2), CdSO(4), PbNO(3), CuSO(4), and Na(2)HAsO(4.) The colony-forming units counted decreased in number from about 150 g(-1) on the heavy-metal-free medium to zero units on media with 40–100 mg l(-1) of HgCl(2), CdSO(4), PbNO(3), or Na(2)HAsO(4). On media with CuSO(4) or ZnSO(4) on the other hand, numbers increased first reaching maxima on media with 50 mg l(-1) CuSO(4) and 90 mg l(-1) ZnSO(4). Higher concentrations reduced the numbers, which however, still remained considerable. Pure microbial isolates in cultures tolerated 200–1600 mg l(-1) of HgCl(2), CdSO(4), PbNO(3), CuSO(4), and Na(2)HAsO(4) in the absence of crude oil. In the presence of oil vapor, the isolates tolerated much lower concentrations of the heavy metals, only 10–80 mg l(-1). The addition of 10 Fe(2)(SO(4))(3) and 200 mg l(-1) proline (by up to two- to threefold) enhanced the tolerance of several isolates to heavy metals, and consequently their potential for oil biodegradation in their presence. The results are useful in designing bioremediation technologies for oil spilled in hypersaline areas. Frontiers Media S.A. 2018-03-07 /pmc/articles/PMC5845895/ /pubmed/29563904 http://dx.doi.org/10.3389/fmicb.2018.00394 Text en Copyright © 2018 Al-Mailem, Eliyas and Radwan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Al-Mailem, Dina M.
Eliyas, Mohamed
Radwan, Samir S.
Ferric Sulfate and Proline Enhance Heavy-Metal Tolerance of Halophilic/Halotolerant Soil Microorganisms and Their Bioremediation Potential for Spilled-Oil Under Multiple Stresses
title Ferric Sulfate and Proline Enhance Heavy-Metal Tolerance of Halophilic/Halotolerant Soil Microorganisms and Their Bioremediation Potential for Spilled-Oil Under Multiple Stresses
title_full Ferric Sulfate and Proline Enhance Heavy-Metal Tolerance of Halophilic/Halotolerant Soil Microorganisms and Their Bioremediation Potential for Spilled-Oil Under Multiple Stresses
title_fullStr Ferric Sulfate and Proline Enhance Heavy-Metal Tolerance of Halophilic/Halotolerant Soil Microorganisms and Their Bioremediation Potential for Spilled-Oil Under Multiple Stresses
title_full_unstemmed Ferric Sulfate and Proline Enhance Heavy-Metal Tolerance of Halophilic/Halotolerant Soil Microorganisms and Their Bioremediation Potential for Spilled-Oil Under Multiple Stresses
title_short Ferric Sulfate and Proline Enhance Heavy-Metal Tolerance of Halophilic/Halotolerant Soil Microorganisms and Their Bioremediation Potential for Spilled-Oil Under Multiple Stresses
title_sort ferric sulfate and proline enhance heavy-metal tolerance of halophilic/halotolerant soil microorganisms and their bioremediation potential for spilled-oil under multiple stresses
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5845895/
https://www.ncbi.nlm.nih.gov/pubmed/29563904
http://dx.doi.org/10.3389/fmicb.2018.00394
work_keys_str_mv AT almailemdinam ferricsulfateandprolineenhanceheavymetaltoleranceofhalophilichalotolerantsoilmicroorganismsandtheirbioremediationpotentialforspilledoilundermultiplestresses
AT eliyasmohamed ferricsulfateandprolineenhanceheavymetaltoleranceofhalophilichalotolerantsoilmicroorganismsandtheirbioremediationpotentialforspilledoilundermultiplestresses
AT radwansamirs ferricsulfateandprolineenhanceheavymetaltoleranceofhalophilichalotolerantsoilmicroorganismsandtheirbioremediationpotentialforspilledoilundermultiplestresses