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Activation of CD4 and CD8 T cell receptors and regulatory T cells in response to human proteins

This study assessed in detail the influence of four different human proteins on the activation of CD4+ and CD8+ T lymphocytes and on the formation of regulatory T cells. Human whole-blood samples were incubated with four different human proteins. The effects of these proteins on the downstream immun...

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Autor principal: Arneth, Borros M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5846456/
https://www.ncbi.nlm.nih.gov/pubmed/29568705
http://dx.doi.org/10.7717/peerj.4462
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author Arneth, Borros M.
author_facet Arneth, Borros M.
author_sort Arneth, Borros M.
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description This study assessed in detail the influence of four different human proteins on the activation of CD4+ and CD8+ T lymphocytes and on the formation of regulatory T cells. Human whole-blood samples were incubated with four different human proteins. The effects of these proteins on the downstream immune-system response, on the expression of extracellular activation markers on and intracellular cytokines in T lymphocytes, and on the number of regulatory T cells (T-reg cells) were investigated via flow cytometry. Incubation with β-actin or glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which are cytoplasmic proteins, increased the expression of both extracellular activation markers (CD69 and HLA-DR) and intracellular cytokines but did not significantly affect the number of T-reg cells. In contrast, incubation with human albumin or insulin, which are serum proteins, reduced both extracellular activation markers and intracellular cytokine expression and subsequently increased the number of T-reg cells. These findings may help to explain the etiological basis of autoimmune diseases.
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spelling pubmed-58464562018-03-22 Activation of CD4 and CD8 T cell receptors and regulatory T cells in response to human proteins Arneth, Borros M. PeerJ Allergy and Clinical Immunology This study assessed in detail the influence of four different human proteins on the activation of CD4+ and CD8+ T lymphocytes and on the formation of regulatory T cells. Human whole-blood samples were incubated with four different human proteins. The effects of these proteins on the downstream immune-system response, on the expression of extracellular activation markers on and intracellular cytokines in T lymphocytes, and on the number of regulatory T cells (T-reg cells) were investigated via flow cytometry. Incubation with β-actin or glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which are cytoplasmic proteins, increased the expression of both extracellular activation markers (CD69 and HLA-DR) and intracellular cytokines but did not significantly affect the number of T-reg cells. In contrast, incubation with human albumin or insulin, which are serum proteins, reduced both extracellular activation markers and intracellular cytokine expression and subsequently increased the number of T-reg cells. These findings may help to explain the etiological basis of autoimmune diseases. PeerJ Inc. 2018-03-09 /pmc/articles/PMC5846456/ /pubmed/29568705 http://dx.doi.org/10.7717/peerj.4462 Text en ©2018 Arneth http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Allergy and Clinical Immunology
Arneth, Borros M.
Activation of CD4 and CD8 T cell receptors and regulatory T cells in response to human proteins
title Activation of CD4 and CD8 T cell receptors and regulatory T cells in response to human proteins
title_full Activation of CD4 and CD8 T cell receptors and regulatory T cells in response to human proteins
title_fullStr Activation of CD4 and CD8 T cell receptors and regulatory T cells in response to human proteins
title_full_unstemmed Activation of CD4 and CD8 T cell receptors and regulatory T cells in response to human proteins
title_short Activation of CD4 and CD8 T cell receptors and regulatory T cells in response to human proteins
title_sort activation of cd4 and cd8 t cell receptors and regulatory t cells in response to human proteins
topic Allergy and Clinical Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5846456/
https://www.ncbi.nlm.nih.gov/pubmed/29568705
http://dx.doi.org/10.7717/peerj.4462
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