Cargando…
Activation of CD4 and CD8 T cell receptors and regulatory T cells in response to human proteins
This study assessed in detail the influence of four different human proteins on the activation of CD4+ and CD8+ T lymphocytes and on the formation of regulatory T cells. Human whole-blood samples were incubated with four different human proteins. The effects of these proteins on the downstream immun...
Autor principal: | |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5846456/ https://www.ncbi.nlm.nih.gov/pubmed/29568705 http://dx.doi.org/10.7717/peerj.4462 |
_version_ | 1783305595180285952 |
---|---|
author | Arneth, Borros M. |
author_facet | Arneth, Borros M. |
author_sort | Arneth, Borros M. |
collection | PubMed |
description | This study assessed in detail the influence of four different human proteins on the activation of CD4+ and CD8+ T lymphocytes and on the formation of regulatory T cells. Human whole-blood samples were incubated with four different human proteins. The effects of these proteins on the downstream immune-system response, on the expression of extracellular activation markers on and intracellular cytokines in T lymphocytes, and on the number of regulatory T cells (T-reg cells) were investigated via flow cytometry. Incubation with β-actin or glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which are cytoplasmic proteins, increased the expression of both extracellular activation markers (CD69 and HLA-DR) and intracellular cytokines but did not significantly affect the number of T-reg cells. In contrast, incubation with human albumin or insulin, which are serum proteins, reduced both extracellular activation markers and intracellular cytokine expression and subsequently increased the number of T-reg cells. These findings may help to explain the etiological basis of autoimmune diseases. |
format | Online Article Text |
id | pubmed-5846456 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58464562018-03-22 Activation of CD4 and CD8 T cell receptors and regulatory T cells in response to human proteins Arneth, Borros M. PeerJ Allergy and Clinical Immunology This study assessed in detail the influence of four different human proteins on the activation of CD4+ and CD8+ T lymphocytes and on the formation of regulatory T cells. Human whole-blood samples were incubated with four different human proteins. The effects of these proteins on the downstream immune-system response, on the expression of extracellular activation markers on and intracellular cytokines in T lymphocytes, and on the number of regulatory T cells (T-reg cells) were investigated via flow cytometry. Incubation with β-actin or glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which are cytoplasmic proteins, increased the expression of both extracellular activation markers (CD69 and HLA-DR) and intracellular cytokines but did not significantly affect the number of T-reg cells. In contrast, incubation with human albumin or insulin, which are serum proteins, reduced both extracellular activation markers and intracellular cytokine expression and subsequently increased the number of T-reg cells. These findings may help to explain the etiological basis of autoimmune diseases. PeerJ Inc. 2018-03-09 /pmc/articles/PMC5846456/ /pubmed/29568705 http://dx.doi.org/10.7717/peerj.4462 Text en ©2018 Arneth http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Allergy and Clinical Immunology Arneth, Borros M. Activation of CD4 and CD8 T cell receptors and regulatory T cells in response to human proteins |
title | Activation of CD4 and CD8 T cell receptors and regulatory T cells in response to human proteins |
title_full | Activation of CD4 and CD8 T cell receptors and regulatory T cells in response to human proteins |
title_fullStr | Activation of CD4 and CD8 T cell receptors and regulatory T cells in response to human proteins |
title_full_unstemmed | Activation of CD4 and CD8 T cell receptors and regulatory T cells in response to human proteins |
title_short | Activation of CD4 and CD8 T cell receptors and regulatory T cells in response to human proteins |
title_sort | activation of cd4 and cd8 t cell receptors and regulatory t cells in response to human proteins |
topic | Allergy and Clinical Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5846456/ https://www.ncbi.nlm.nih.gov/pubmed/29568705 http://dx.doi.org/10.7717/peerj.4462 |
work_keys_str_mv | AT arnethborrosm activationofcd4andcd8tcellreceptorsandregulatorytcellsinresponsetohumanproteins |