Cargando…
Transplantation of allogenic nucleus pulposus cells attenuates intervertebral disc degeneration by inhibiting apoptosis and increasing migration
Transplantation of nucleus pulposus cells (NPCs) into the intervertebral disc (IVD) has been demonstrated to be an effective treatment of degenerative disc disease (DDD). However, the underlying mechanisms have remained to be sufficiently elucidated. The aim of the present study was to explore the p...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5846671/ https://www.ncbi.nlm.nih.gov/pubmed/29436582 http://dx.doi.org/10.3892/ijmm.2018.3454 |
_version_ | 1783305609286778880 |
---|---|
author | Wang, Weiheng Deng, Guoying Qiu, Yuanyuan Huang, Xiaodong Xi, Yanhai Yu, Jiangming Yang, Xiangqun Ye, Xiaojian |
author_facet | Wang, Weiheng Deng, Guoying Qiu, Yuanyuan Huang, Xiaodong Xi, Yanhai Yu, Jiangming Yang, Xiangqun Ye, Xiaojian |
author_sort | Wang, Weiheng |
collection | PubMed |
description | Transplantation of nucleus pulposus cells (NPCs) into the intervertebral disc (IVD) has been demonstrated to be an effective treatment of degenerative disc disease (DDD). However, the underlying mechanisms have remained to be sufficiently elucidated. The aim of the present study was to explore the potential cell migration and anti-apoptosis efficacy of NPCs in the treatment of DDD. NPCs cultured from rats expressing green fluorescent protein (GFP-NPCs) were transplanted into the degenerated IVD, and the migration of GFP-NPCs, as well as the degeneration and apoptosis of the IVD were detected to evaluate the therapeutic effect in vivo. In vitro, disc chondrocytes (DCs) and annulus fibrosus cells (AFCs) were co-cultured to explore the underlying mechanism. The results demonstrated that injection of NPCs suppressed DDD by inhibiting apoptosis and increasing extracellular matrix in vivo and in vitro. NPCs migrated into the inner AF in vivo, and NPC migration was observed to be promoted by AFCs and DCs in vitro, particularly by damaged AFCs. These results demonstrated the anti-apoptotic effects and migratory capacity of allogenic NPCs transplanted into the IVD, which evidences the contribution of NPCs to disc regeneration and provide a novel strategy for treating DDD. |
format | Online Article Text |
id | pubmed-5846671 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-58466712018-03-20 Transplantation of allogenic nucleus pulposus cells attenuates intervertebral disc degeneration by inhibiting apoptosis and increasing migration Wang, Weiheng Deng, Guoying Qiu, Yuanyuan Huang, Xiaodong Xi, Yanhai Yu, Jiangming Yang, Xiangqun Ye, Xiaojian Int J Mol Med Articles Transplantation of nucleus pulposus cells (NPCs) into the intervertebral disc (IVD) has been demonstrated to be an effective treatment of degenerative disc disease (DDD). However, the underlying mechanisms have remained to be sufficiently elucidated. The aim of the present study was to explore the potential cell migration and anti-apoptosis efficacy of NPCs in the treatment of DDD. NPCs cultured from rats expressing green fluorescent protein (GFP-NPCs) were transplanted into the degenerated IVD, and the migration of GFP-NPCs, as well as the degeneration and apoptosis of the IVD were detected to evaluate the therapeutic effect in vivo. In vitro, disc chondrocytes (DCs) and annulus fibrosus cells (AFCs) were co-cultured to explore the underlying mechanism. The results demonstrated that injection of NPCs suppressed DDD by inhibiting apoptosis and increasing extracellular matrix in vivo and in vitro. NPCs migrated into the inner AF in vivo, and NPC migration was observed to be promoted by AFCs and DCs in vitro, particularly by damaged AFCs. These results demonstrated the anti-apoptotic effects and migratory capacity of allogenic NPCs transplanted into the IVD, which evidences the contribution of NPCs to disc regeneration and provide a novel strategy for treating DDD. D.A. Spandidos 2018-05 2018-02-02 /pmc/articles/PMC5846671/ /pubmed/29436582 http://dx.doi.org/10.3892/ijmm.2018.3454 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Wang, Weiheng Deng, Guoying Qiu, Yuanyuan Huang, Xiaodong Xi, Yanhai Yu, Jiangming Yang, Xiangqun Ye, Xiaojian Transplantation of allogenic nucleus pulposus cells attenuates intervertebral disc degeneration by inhibiting apoptosis and increasing migration |
title | Transplantation of allogenic nucleus pulposus cells attenuates intervertebral disc degeneration by inhibiting apoptosis and increasing migration |
title_full | Transplantation of allogenic nucleus pulposus cells attenuates intervertebral disc degeneration by inhibiting apoptosis and increasing migration |
title_fullStr | Transplantation of allogenic nucleus pulposus cells attenuates intervertebral disc degeneration by inhibiting apoptosis and increasing migration |
title_full_unstemmed | Transplantation of allogenic nucleus pulposus cells attenuates intervertebral disc degeneration by inhibiting apoptosis and increasing migration |
title_short | Transplantation of allogenic nucleus pulposus cells attenuates intervertebral disc degeneration by inhibiting apoptosis and increasing migration |
title_sort | transplantation of allogenic nucleus pulposus cells attenuates intervertebral disc degeneration by inhibiting apoptosis and increasing migration |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5846671/ https://www.ncbi.nlm.nih.gov/pubmed/29436582 http://dx.doi.org/10.3892/ijmm.2018.3454 |
work_keys_str_mv | AT wangweiheng transplantationofallogenicnucleuspulposuscellsattenuatesintervertebraldiscdegenerationbyinhibitingapoptosisandincreasingmigration AT dengguoying transplantationofallogenicnucleuspulposuscellsattenuatesintervertebraldiscdegenerationbyinhibitingapoptosisandincreasingmigration AT qiuyuanyuan transplantationofallogenicnucleuspulposuscellsattenuatesintervertebraldiscdegenerationbyinhibitingapoptosisandincreasingmigration AT huangxiaodong transplantationofallogenicnucleuspulposuscellsattenuatesintervertebraldiscdegenerationbyinhibitingapoptosisandincreasingmigration AT xiyanhai transplantationofallogenicnucleuspulposuscellsattenuatesintervertebraldiscdegenerationbyinhibitingapoptosisandincreasingmigration AT yujiangming transplantationofallogenicnucleuspulposuscellsattenuatesintervertebraldiscdegenerationbyinhibitingapoptosisandincreasingmigration AT yangxiangqun transplantationofallogenicnucleuspulposuscellsattenuatesintervertebraldiscdegenerationbyinhibitingapoptosisandincreasingmigration AT yexiaojian transplantationofallogenicnucleuspulposuscellsattenuatesintervertebraldiscdegenerationbyinhibitingapoptosisandincreasingmigration |