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A T‐cell diagnostic test for cystic echinococcosis based on Antigen B peptides
Cystic echinococcosis (CE) immunodiagnosis is still imperfect. We recently set‐up a whole‐blood test based on the interleukin (IL)‐4 response to the native Antigen B (AgB) of Echinococcus granulosus. However, AgB is encoded by a multigene family coding for five putative subunits. Therefore, the aims...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5846893/ https://www.ncbi.nlm.nih.gov/pubmed/29171068 http://dx.doi.org/10.1111/pim.12499 |
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author | Petrone, L. Vanini, V. Amicosante, M. Corpolongo, A. Gomez Morales, M. A. Ludovisi, A. Ippolito, G. Pozio, E. Teggi, A. Goletti, D. |
author_facet | Petrone, L. Vanini, V. Amicosante, M. Corpolongo, A. Gomez Morales, M. A. Ludovisi, A. Ippolito, G. Pozio, E. Teggi, A. Goletti, D. |
author_sort | Petrone, L. |
collection | PubMed |
description | Cystic echinococcosis (CE) immunodiagnosis is still imperfect. We recently set‐up a whole‐blood test based on the interleukin (IL)‐4 response to the native Antigen B (AgB) of Echinococcus granulosus. However, AgB is encoded by a multigene family coding for five putative subunits. Therefore, the aims of this study were to analyse the IL‐4 response to peptides spanning the immunodominant regions of the five AgB subunits and to evaluate the accuracy of this assay for CE diagnosis. Peptides corresponding to each subunit were combined into five pools. A pool containing all peptides was also used (total pool). IL‐4 evaluated by enzyme‐linked immunosorbent assay was significantly higher in patients with CE compared to those without (NO‐CE subjects) when whole‐blood was stimulated with AgB1 and with the total pool. Moreover, IL‐4 levels in response to the total pool were significantly increased in patients with active cysts. Receiver Operator Curve analysis identified a cut‐off point of 0.59 pg/mL predicting active cysts diagnosis with 71% sensitivity and 82% specificity in serology‐positive CE patients. These data, if confirmed in a larger cohort, offer the opportunity to develop new diagnostic tools for CE based on a standardized source of AgB as the peptides. |
format | Online Article Text |
id | pubmed-5846893 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58468932018-03-20 A T‐cell diagnostic test for cystic echinococcosis based on Antigen B peptides Petrone, L. Vanini, V. Amicosante, M. Corpolongo, A. Gomez Morales, M. A. Ludovisi, A. Ippolito, G. Pozio, E. Teggi, A. Goletti, D. Parasite Immunol Original Articles Cystic echinococcosis (CE) immunodiagnosis is still imperfect. We recently set‐up a whole‐blood test based on the interleukin (IL)‐4 response to the native Antigen B (AgB) of Echinococcus granulosus. However, AgB is encoded by a multigene family coding for five putative subunits. Therefore, the aims of this study were to analyse the IL‐4 response to peptides spanning the immunodominant regions of the five AgB subunits and to evaluate the accuracy of this assay for CE diagnosis. Peptides corresponding to each subunit were combined into five pools. A pool containing all peptides was also used (total pool). IL‐4 evaluated by enzyme‐linked immunosorbent assay was significantly higher in patients with CE compared to those without (NO‐CE subjects) when whole‐blood was stimulated with AgB1 and with the total pool. Moreover, IL‐4 levels in response to the total pool were significantly increased in patients with active cysts. Receiver Operator Curve analysis identified a cut‐off point of 0.59 pg/mL predicting active cysts diagnosis with 71% sensitivity and 82% specificity in serology‐positive CE patients. These data, if confirmed in a larger cohort, offer the opportunity to develop new diagnostic tools for CE based on a standardized source of AgB as the peptides. John Wiley and Sons Inc. 2017-11-24 2017-12 /pmc/articles/PMC5846893/ /pubmed/29171068 http://dx.doi.org/10.1111/pim.12499 Text en © 2017 The Authors. Parasite Immunology Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Petrone, L. Vanini, V. Amicosante, M. Corpolongo, A. Gomez Morales, M. A. Ludovisi, A. Ippolito, G. Pozio, E. Teggi, A. Goletti, D. A T‐cell diagnostic test for cystic echinococcosis based on Antigen B peptides |
title | A T‐cell diagnostic test for cystic echinococcosis based on Antigen B peptides |
title_full | A T‐cell diagnostic test for cystic echinococcosis based on Antigen B peptides |
title_fullStr | A T‐cell diagnostic test for cystic echinococcosis based on Antigen B peptides |
title_full_unstemmed | A T‐cell diagnostic test for cystic echinococcosis based on Antigen B peptides |
title_short | A T‐cell diagnostic test for cystic echinococcosis based on Antigen B peptides |
title_sort | t‐cell diagnostic test for cystic echinococcosis based on antigen b peptides |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5846893/ https://www.ncbi.nlm.nih.gov/pubmed/29171068 http://dx.doi.org/10.1111/pim.12499 |
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