Cargando…

A T‐cell diagnostic test for cystic echinococcosis based on Antigen B peptides

Cystic echinococcosis (CE) immunodiagnosis is still imperfect. We recently set‐up a whole‐blood test based on the interleukin (IL)‐4 response to the native Antigen B (AgB) of Echinococcus granulosus. However, AgB is encoded by a multigene family coding for five putative subunits. Therefore, the aims...

Descripción completa

Detalles Bibliográficos
Autores principales: Petrone, L., Vanini, V., Amicosante, M., Corpolongo, A., Gomez Morales, M. A., Ludovisi, A., Ippolito, G., Pozio, E., Teggi, A., Goletti, D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5846893/
https://www.ncbi.nlm.nih.gov/pubmed/29171068
http://dx.doi.org/10.1111/pim.12499
_version_ 1783305652606599168
author Petrone, L.
Vanini, V.
Amicosante, M.
Corpolongo, A.
Gomez Morales, M. A.
Ludovisi, A.
Ippolito, G.
Pozio, E.
Teggi, A.
Goletti, D.
author_facet Petrone, L.
Vanini, V.
Amicosante, M.
Corpolongo, A.
Gomez Morales, M. A.
Ludovisi, A.
Ippolito, G.
Pozio, E.
Teggi, A.
Goletti, D.
author_sort Petrone, L.
collection PubMed
description Cystic echinococcosis (CE) immunodiagnosis is still imperfect. We recently set‐up a whole‐blood test based on the interleukin (IL)‐4 response to the native Antigen B (AgB) of Echinococcus granulosus. However, AgB is encoded by a multigene family coding for five putative subunits. Therefore, the aims of this study were to analyse the IL‐4 response to peptides spanning the immunodominant regions of the five AgB subunits and to evaluate the accuracy of this assay for CE diagnosis. Peptides corresponding to each subunit were combined into five pools. A pool containing all peptides was also used (total pool). IL‐4 evaluated by enzyme‐linked immunosorbent assay was significantly higher in patients with CE compared to those without (NO‐CE subjects) when whole‐blood was stimulated with AgB1 and with the total pool. Moreover, IL‐4 levels in response to the total pool were significantly increased in patients with active cysts. Receiver Operator Curve analysis identified a cut‐off point of 0.59 pg/mL predicting active cysts diagnosis with 71% sensitivity and 82% specificity in serology‐positive CE patients. These data, if confirmed in a larger cohort, offer the opportunity to develop new diagnostic tools for CE based on a standardized source of AgB as the peptides.
format Online
Article
Text
id pubmed-5846893
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-58468932018-03-20 A T‐cell diagnostic test for cystic echinococcosis based on Antigen B peptides Petrone, L. Vanini, V. Amicosante, M. Corpolongo, A. Gomez Morales, M. A. Ludovisi, A. Ippolito, G. Pozio, E. Teggi, A. Goletti, D. Parasite Immunol Original Articles Cystic echinococcosis (CE) immunodiagnosis is still imperfect. We recently set‐up a whole‐blood test based on the interleukin (IL)‐4 response to the native Antigen B (AgB) of Echinococcus granulosus. However, AgB is encoded by a multigene family coding for five putative subunits. Therefore, the aims of this study were to analyse the IL‐4 response to peptides spanning the immunodominant regions of the five AgB subunits and to evaluate the accuracy of this assay for CE diagnosis. Peptides corresponding to each subunit were combined into five pools. A pool containing all peptides was also used (total pool). IL‐4 evaluated by enzyme‐linked immunosorbent assay was significantly higher in patients with CE compared to those without (NO‐CE subjects) when whole‐blood was stimulated with AgB1 and with the total pool. Moreover, IL‐4 levels in response to the total pool were significantly increased in patients with active cysts. Receiver Operator Curve analysis identified a cut‐off point of 0.59 pg/mL predicting active cysts diagnosis with 71% sensitivity and 82% specificity in serology‐positive CE patients. These data, if confirmed in a larger cohort, offer the opportunity to develop new diagnostic tools for CE based on a standardized source of AgB as the peptides. John Wiley and Sons Inc. 2017-11-24 2017-12 /pmc/articles/PMC5846893/ /pubmed/29171068 http://dx.doi.org/10.1111/pim.12499 Text en © 2017 The Authors. Parasite Immunology Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Petrone, L.
Vanini, V.
Amicosante, M.
Corpolongo, A.
Gomez Morales, M. A.
Ludovisi, A.
Ippolito, G.
Pozio, E.
Teggi, A.
Goletti, D.
A T‐cell diagnostic test for cystic echinococcosis based on Antigen B peptides
title A T‐cell diagnostic test for cystic echinococcosis based on Antigen B peptides
title_full A T‐cell diagnostic test for cystic echinococcosis based on Antigen B peptides
title_fullStr A T‐cell diagnostic test for cystic echinococcosis based on Antigen B peptides
title_full_unstemmed A T‐cell diagnostic test for cystic echinococcosis based on Antigen B peptides
title_short A T‐cell diagnostic test for cystic echinococcosis based on Antigen B peptides
title_sort t‐cell diagnostic test for cystic echinococcosis based on antigen b peptides
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5846893/
https://www.ncbi.nlm.nih.gov/pubmed/29171068
http://dx.doi.org/10.1111/pim.12499
work_keys_str_mv AT petronel atcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides
AT vaniniv atcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides
AT amicosantem atcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides
AT corpolongoa atcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides
AT gomezmoralesma atcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides
AT ludovisia atcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides
AT ippolitog atcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides
AT pozioe atcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides
AT teggia atcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides
AT golettid atcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides
AT petronel tcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides
AT vaniniv tcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides
AT amicosantem tcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides
AT corpolongoa tcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides
AT gomezmoralesma tcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides
AT ludovisia tcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides
AT ippolitog tcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides
AT pozioe tcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides
AT teggia tcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides
AT golettid tcelldiagnostictestforcysticechinococcosisbasedonantigenbpeptides