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Heart irradiation reduces microvascular density and accumulation of HSPA1 in mice

PURPOSE: Improvement of radiotherapy techniques reduces the exposure of normal tissues to ionizing radiation. However, the risk of radiation-related late effects remains elevated. In the present study, we investigated long-term effects of radiation on heart muscle morphology. MATERIALS AND METHODS:...

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Autores principales: Walaszczyk, Anna, Szołtysek, Katarzyna, Jelonek, Karol, Polańska, Joanna, Dörr, Wolfgang, Haagen, Julia, Widłak, Piotr, Gabryś, Dorota
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847036/
https://www.ncbi.nlm.nih.gov/pubmed/29063166
http://dx.doi.org/10.1007/s00066-017-1220-z
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author Walaszczyk, Anna
Szołtysek, Katarzyna
Jelonek, Karol
Polańska, Joanna
Dörr, Wolfgang
Haagen, Julia
Widłak, Piotr
Gabryś, Dorota
author_facet Walaszczyk, Anna
Szołtysek, Katarzyna
Jelonek, Karol
Polańska, Joanna
Dörr, Wolfgang
Haagen, Julia
Widłak, Piotr
Gabryś, Dorota
author_sort Walaszczyk, Anna
collection PubMed
description PURPOSE: Improvement of radiotherapy techniques reduces the exposure of normal tissues to ionizing radiation. However, the risk of radiation-related late effects remains elevated. In the present study, we investigated long-term effects of radiation on heart muscle morphology. MATERIALS AND METHODS: We established a mouse model to study microvascular density (MVD), deposition of collagen fibers, and changes in accumulation of heat shock 70 kDa protein 1 (HSPA1) in irradiated heart tissue. Hearts of C57BL/6 mice received a single dose of X‑ray radiation in the range 0.2–16 Gy. Analyses were performed 20, 40, and 60 weeks after irradiation. RESULTS: Reduction in MD was revealed as a long-term effect observed 20–60 weeks after irradiation. Moreover, a significant and dose-dependent increase in accumulation of HSPA1, both cytoplasmic and nuclear, was observed in heart tissues collected 20 weeks after irradiation. We also noticed an increase in collagen deposition in hearts treated with higher doses. CONCLUSIONS: This study shows that some changes induced by radiation in the heart tissue, such as reduction in microvessel density, increase in collagen deposition, and accumulation of HSPA1, are observed as long-term effects which might be associated with late radiation cardiotoxicity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00066-017-1220-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-58470362018-03-20 Heart irradiation reduces microvascular density and accumulation of HSPA1 in mice Walaszczyk, Anna Szołtysek, Katarzyna Jelonek, Karol Polańska, Joanna Dörr, Wolfgang Haagen, Julia Widłak, Piotr Gabryś, Dorota Strahlenther Onkol Original Article PURPOSE: Improvement of radiotherapy techniques reduces the exposure of normal tissues to ionizing radiation. However, the risk of radiation-related late effects remains elevated. In the present study, we investigated long-term effects of radiation on heart muscle morphology. MATERIALS AND METHODS: We established a mouse model to study microvascular density (MVD), deposition of collagen fibers, and changes in accumulation of heat shock 70 kDa protein 1 (HSPA1) in irradiated heart tissue. Hearts of C57BL/6 mice received a single dose of X‑ray radiation in the range 0.2–16 Gy. Analyses were performed 20, 40, and 60 weeks after irradiation. RESULTS: Reduction in MD was revealed as a long-term effect observed 20–60 weeks after irradiation. Moreover, a significant and dose-dependent increase in accumulation of HSPA1, both cytoplasmic and nuclear, was observed in heart tissues collected 20 weeks after irradiation. We also noticed an increase in collagen deposition in hearts treated with higher doses. CONCLUSIONS: This study shows that some changes induced by radiation in the heart tissue, such as reduction in microvessel density, increase in collagen deposition, and accumulation of HSPA1, are observed as long-term effects which might be associated with late radiation cardiotoxicity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00066-017-1220-z) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2017-10-23 2018 /pmc/articles/PMC5847036/ /pubmed/29063166 http://dx.doi.org/10.1007/s00066-017-1220-z Text en © The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Walaszczyk, Anna
Szołtysek, Katarzyna
Jelonek, Karol
Polańska, Joanna
Dörr, Wolfgang
Haagen, Julia
Widłak, Piotr
Gabryś, Dorota
Heart irradiation reduces microvascular density and accumulation of HSPA1 in mice
title Heart irradiation reduces microvascular density and accumulation of HSPA1 in mice
title_full Heart irradiation reduces microvascular density and accumulation of HSPA1 in mice
title_fullStr Heart irradiation reduces microvascular density and accumulation of HSPA1 in mice
title_full_unstemmed Heart irradiation reduces microvascular density and accumulation of HSPA1 in mice
title_short Heart irradiation reduces microvascular density and accumulation of HSPA1 in mice
title_sort heart irradiation reduces microvascular density and accumulation of hspa1 in mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847036/
https://www.ncbi.nlm.nih.gov/pubmed/29063166
http://dx.doi.org/10.1007/s00066-017-1220-z
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