Cargando…

A Dual Reporter Iodinated Labeling Reagent for Cancer Positron Emission Tomography Imaging and Fluorescence-Guided Surgery

[Image: see text] The combination of early diagnosis and complete surgical resection offers the greatest prospect of curative cancer treatment. An iodine-124/fluorescein-based dual-modality labeling reagent, (124)I-Green, constitutes a generic tool for one-step installation of a positron emission to...

Descripción completa

Detalles Bibliográficos
Autores principales: Lu, Zhi, Pham, Truc Thuy, Rajkumar, Vineeth, Yu, Zilin, Pedley, R Barbara, Årstad, Erik, Maher, John, Yan, Ran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847118/
https://www.ncbi.nlm.nih.gov/pubmed/29388770
http://dx.doi.org/10.1021/acs.jmedchem.7b01746
_version_ 1783305688256086016
author Lu, Zhi
Pham, Truc Thuy
Rajkumar, Vineeth
Yu, Zilin
Pedley, R Barbara
Årstad, Erik
Maher, John
Yan, Ran
author_facet Lu, Zhi
Pham, Truc Thuy
Rajkumar, Vineeth
Yu, Zilin
Pedley, R Barbara
Årstad, Erik
Maher, John
Yan, Ran
author_sort Lu, Zhi
collection PubMed
description [Image: see text] The combination of early diagnosis and complete surgical resection offers the greatest prospect of curative cancer treatment. An iodine-124/fluorescein-based dual-modality labeling reagent, (124)I-Green, constitutes a generic tool for one-step installation of a positron emission tomography (PET) and a fluorescent reporter to any cancer-specific antibody. The resulting antibody conjugate would allow both cancer PET imaging and intraoperative fluorescence-guided surgery. (124)I-Green was synthesized in excellent radiochemical yields of 92 ± 5% (n = 4) determined by HPLC with an improved one-pot three-component radioiodination reaction. The A5B7 carcinoembryonic antigen (CEA)-specific antibody was conjugated to (124)I-Green. High tumor uptake of the dual-labeled A5B7 of 20.21 ± 2.70, 13.31 ± 0.73, and 10.64 ± 1.86%ID/g was observed in CEA-expressing SW1222 xenograft mouse model (n = 3) at 24, 48, and 72 h post intravenous injection, respectively. The xenografts were clearly visualized by both PET/CT and ex vivo fluorescence imaging. These encouraging results warrant the further translational development of (124)I-Green for cancer PET imaging and fluorescence-guided surgery.
format Online
Article
Text
id pubmed-5847118
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-58471182018-03-13 A Dual Reporter Iodinated Labeling Reagent for Cancer Positron Emission Tomography Imaging and Fluorescence-Guided Surgery Lu, Zhi Pham, Truc Thuy Rajkumar, Vineeth Yu, Zilin Pedley, R Barbara Årstad, Erik Maher, John Yan, Ran J Med Chem [Image: see text] The combination of early diagnosis and complete surgical resection offers the greatest prospect of curative cancer treatment. An iodine-124/fluorescein-based dual-modality labeling reagent, (124)I-Green, constitutes a generic tool for one-step installation of a positron emission tomography (PET) and a fluorescent reporter to any cancer-specific antibody. The resulting antibody conjugate would allow both cancer PET imaging and intraoperative fluorescence-guided surgery. (124)I-Green was synthesized in excellent radiochemical yields of 92 ± 5% (n = 4) determined by HPLC with an improved one-pot three-component radioiodination reaction. The A5B7 carcinoembryonic antigen (CEA)-specific antibody was conjugated to (124)I-Green. High tumor uptake of the dual-labeled A5B7 of 20.21 ± 2.70, 13.31 ± 0.73, and 10.64 ± 1.86%ID/g was observed in CEA-expressing SW1222 xenograft mouse model (n = 3) at 24, 48, and 72 h post intravenous injection, respectively. The xenografts were clearly visualized by both PET/CT and ex vivo fluorescence imaging. These encouraging results warrant the further translational development of (124)I-Green for cancer PET imaging and fluorescence-guided surgery. American Chemical Society 2018-02-01 2018-02-22 /pmc/articles/PMC5847118/ /pubmed/29388770 http://dx.doi.org/10.1021/acs.jmedchem.7b01746 Text en Copyright © 2018 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
spellingShingle Lu, Zhi
Pham, Truc Thuy
Rajkumar, Vineeth
Yu, Zilin
Pedley, R Barbara
Årstad, Erik
Maher, John
Yan, Ran
A Dual Reporter Iodinated Labeling Reagent for Cancer Positron Emission Tomography Imaging and Fluorescence-Guided Surgery
title A Dual Reporter Iodinated Labeling Reagent for Cancer Positron Emission Tomography Imaging and Fluorescence-Guided Surgery
title_full A Dual Reporter Iodinated Labeling Reagent for Cancer Positron Emission Tomography Imaging and Fluorescence-Guided Surgery
title_fullStr A Dual Reporter Iodinated Labeling Reagent for Cancer Positron Emission Tomography Imaging and Fluorescence-Guided Surgery
title_full_unstemmed A Dual Reporter Iodinated Labeling Reagent for Cancer Positron Emission Tomography Imaging and Fluorescence-Guided Surgery
title_short A Dual Reporter Iodinated Labeling Reagent for Cancer Positron Emission Tomography Imaging and Fluorescence-Guided Surgery
title_sort dual reporter iodinated labeling reagent for cancer positron emission tomography imaging and fluorescence-guided surgery
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847118/
https://www.ncbi.nlm.nih.gov/pubmed/29388770
http://dx.doi.org/10.1021/acs.jmedchem.7b01746
work_keys_str_mv AT luzhi adualreporteriodinatedlabelingreagentforcancerpositronemissiontomographyimagingandfluorescenceguidedsurgery
AT phamtructhuy adualreporteriodinatedlabelingreagentforcancerpositronemissiontomographyimagingandfluorescenceguidedsurgery
AT rajkumarvineeth adualreporteriodinatedlabelingreagentforcancerpositronemissiontomographyimagingandfluorescenceguidedsurgery
AT yuzilin adualreporteriodinatedlabelingreagentforcancerpositronemissiontomographyimagingandfluorescenceguidedsurgery
AT pedleyrbarbara adualreporteriodinatedlabelingreagentforcancerpositronemissiontomographyimagingandfluorescenceguidedsurgery
AT arstaderik adualreporteriodinatedlabelingreagentforcancerpositronemissiontomographyimagingandfluorescenceguidedsurgery
AT maherjohn adualreporteriodinatedlabelingreagentforcancerpositronemissiontomographyimagingandfluorescenceguidedsurgery
AT yanran adualreporteriodinatedlabelingreagentforcancerpositronemissiontomographyimagingandfluorescenceguidedsurgery
AT luzhi dualreporteriodinatedlabelingreagentforcancerpositronemissiontomographyimagingandfluorescenceguidedsurgery
AT phamtructhuy dualreporteriodinatedlabelingreagentforcancerpositronemissiontomographyimagingandfluorescenceguidedsurgery
AT rajkumarvineeth dualreporteriodinatedlabelingreagentforcancerpositronemissiontomographyimagingandfluorescenceguidedsurgery
AT yuzilin dualreporteriodinatedlabelingreagentforcancerpositronemissiontomographyimagingandfluorescenceguidedsurgery
AT pedleyrbarbara dualreporteriodinatedlabelingreagentforcancerpositronemissiontomographyimagingandfluorescenceguidedsurgery
AT arstaderik dualreporteriodinatedlabelingreagentforcancerpositronemissiontomographyimagingandfluorescenceguidedsurgery
AT maherjohn dualreporteriodinatedlabelingreagentforcancerpositronemissiontomographyimagingandfluorescenceguidedsurgery
AT yanran dualreporteriodinatedlabelingreagentforcancerpositronemissiontomographyimagingandfluorescenceguidedsurgery