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Genetic characterization of H9N2 avian influenza viruses isolated from poultry in Poland during 2013/2014

The study presents molecular characterization of H9N2 avian influenza (AI) isolates from field outbreaks in turkeys that occurred in Poland in 2013–2014. Sequences of all gene segments of one isolate from 2013 (A/turkey/Poland/14/2013(H9N2)) and two isolates from 2014 (A/turkey/Poland/08/2014(H9N2),...

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Autores principales: Świętoń, Edyta, Jóźwiak, Michał, Minta, Zenon, Śmietanka, Krzysztof
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847159/
https://www.ncbi.nlm.nih.gov/pubmed/29052126
http://dx.doi.org/10.1007/s11262-017-1513-4
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author Świętoń, Edyta
Jóźwiak, Michał
Minta, Zenon
Śmietanka, Krzysztof
author_facet Świętoń, Edyta
Jóźwiak, Michał
Minta, Zenon
Śmietanka, Krzysztof
author_sort Świętoń, Edyta
collection PubMed
description The study presents molecular characterization of H9N2 avian influenza (AI) isolates from field outbreaks in turkeys that occurred in Poland in 2013–2014. Sequences of all gene segments of one isolate from 2013 (A/turkey/Poland/14/2013(H9N2)) and two isolates from 2014 (A/turkey/Poland/08/2014(H9N2), A/turkey/Poland/09/2014(H9N2)) were obtained and analyzed in search of the phylogenetic relationship and molecular markers of zoonotic potential or increased pathogenicity. All gene segments were shown to originate from the wild bird reservoir and the close relationship of the analyzed isolates proved the link between the outbreaks in 2013 and 2014. However, remarkable molecular differences between isolates from 2013 to 2014 were identified, including mutation in the HA cleavage site (CS) leading to conversion from the PAASNR*GLF to the PAASKR*GLF motif and truncation of the PB1-F2 protein. Additionally, T97I substitution in the PA protein in A/turkey/Poland/08/2014 was detected which can be responsible for enhanced activity of viral polymerase in mammalian cells. However, experimental infection of mice with both isolates from 2014 showed their low pathogenicity, and no statistically significant differences in virus replication were observed between the viruses. Nevertheless, these findings indicate the dynamic evolution of H9N2 in the field emphasizing the need for monitoring of the situation in terms of H9N2 AI in Europe. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11262-017-1513-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-58471592018-03-20 Genetic characterization of H9N2 avian influenza viruses isolated from poultry in Poland during 2013/2014 Świętoń, Edyta Jóźwiak, Michał Minta, Zenon Śmietanka, Krzysztof Virus Genes Article The study presents molecular characterization of H9N2 avian influenza (AI) isolates from field outbreaks in turkeys that occurred in Poland in 2013–2014. Sequences of all gene segments of one isolate from 2013 (A/turkey/Poland/14/2013(H9N2)) and two isolates from 2014 (A/turkey/Poland/08/2014(H9N2), A/turkey/Poland/09/2014(H9N2)) were obtained and analyzed in search of the phylogenetic relationship and molecular markers of zoonotic potential or increased pathogenicity. All gene segments were shown to originate from the wild bird reservoir and the close relationship of the analyzed isolates proved the link between the outbreaks in 2013 and 2014. However, remarkable molecular differences between isolates from 2013 to 2014 were identified, including mutation in the HA cleavage site (CS) leading to conversion from the PAASNR*GLF to the PAASKR*GLF motif and truncation of the PB1-F2 protein. Additionally, T97I substitution in the PA protein in A/turkey/Poland/08/2014 was detected which can be responsible for enhanced activity of viral polymerase in mammalian cells. However, experimental infection of mice with both isolates from 2014 showed their low pathogenicity, and no statistically significant differences in virus replication were observed between the viruses. Nevertheless, these findings indicate the dynamic evolution of H9N2 in the field emphasizing the need for monitoring of the situation in terms of H9N2 AI in Europe. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11262-017-1513-4) contains supplementary material, which is available to authorized users. Springer US 2017-10-19 2018 /pmc/articles/PMC5847159/ /pubmed/29052126 http://dx.doi.org/10.1007/s11262-017-1513-4 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Świętoń, Edyta
Jóźwiak, Michał
Minta, Zenon
Śmietanka, Krzysztof
Genetic characterization of H9N2 avian influenza viruses isolated from poultry in Poland during 2013/2014
title Genetic characterization of H9N2 avian influenza viruses isolated from poultry in Poland during 2013/2014
title_full Genetic characterization of H9N2 avian influenza viruses isolated from poultry in Poland during 2013/2014
title_fullStr Genetic characterization of H9N2 avian influenza viruses isolated from poultry in Poland during 2013/2014
title_full_unstemmed Genetic characterization of H9N2 avian influenza viruses isolated from poultry in Poland during 2013/2014
title_short Genetic characterization of H9N2 avian influenza viruses isolated from poultry in Poland during 2013/2014
title_sort genetic characterization of h9n2 avian influenza viruses isolated from poultry in poland during 2013/2014
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847159/
https://www.ncbi.nlm.nih.gov/pubmed/29052126
http://dx.doi.org/10.1007/s11262-017-1513-4
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