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Basophils activated via TLR signaling may contribute to pathophysiology of type 1 autoimmune pancreatitis

BACKGROUND: Pathophysiology of type 1 autoimmune pancreatitis (AIP) is still unclear. We previously reported that M2 macrophages might play an important role in type 1 AIP. Recently, it has been reported that basophils regulate differentiation to M2 macrophages. In this study, we investigated basoph...

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Autores principales: Yanagawa, Masato, Uchida, Kazushige, Ando, Yugo, Tomiyama, Takashi, Yamaguchi, Takashi, Ikeura, Tsukasa, Fukui, Toshiro, Nishio, Akiyoshi, Uemura, Yoshiko, Miyara, Takayuki, Okamoto, Hiroyuki, Satoi, Souhei, Okazaki, Kazuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Japan 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847205/
https://www.ncbi.nlm.nih.gov/pubmed/28921377
http://dx.doi.org/10.1007/s00535-017-1390-6
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author Yanagawa, Masato
Uchida, Kazushige
Ando, Yugo
Tomiyama, Takashi
Yamaguchi, Takashi
Ikeura, Tsukasa
Fukui, Toshiro
Nishio, Akiyoshi
Uemura, Yoshiko
Miyara, Takayuki
Okamoto, Hiroyuki
Satoi, Souhei
Okazaki, Kazuichi
author_facet Yanagawa, Masato
Uchida, Kazushige
Ando, Yugo
Tomiyama, Takashi
Yamaguchi, Takashi
Ikeura, Tsukasa
Fukui, Toshiro
Nishio, Akiyoshi
Uemura, Yoshiko
Miyara, Takayuki
Okamoto, Hiroyuki
Satoi, Souhei
Okazaki, Kazuichi
author_sort Yanagawa, Masato
collection PubMed
description BACKGROUND: Pathophysiology of type 1 autoimmune pancreatitis (AIP) is still unclear. We previously reported that M2 macrophages might play an important role in type 1 AIP. Recently, it has been reported that basophils regulate differentiation to M2 macrophages. In this study, we investigated basophils from the pancreatic tissue and peripheral blood of individuals with type 1 AIP. METHODS: By using immunohistochemistry, we investigated basophils in pancreatic tissue from 13 patients with type 1 AIP and examined expression of toll-like receptors (TLRs) by these cells. Additionally, we obtained peripheral blood samples from 27 healthy subjects, 40 patients with type 1 AIP, 8 patients with alcoholic chronic pancreatitis, 10 patients with bronchial asthma, and 10 patients with atopic dermatitis, and analyzed activation of basophils by stimulating them with ligands of TLR1–9. We also compared TLR expression in basophils from the tissue and blood samples. RESULTS: Basophils were detected in pancreatic tissues from 10 of 13 patients with type 1 AIP. Flow cytometric analysis revealed that the ratios of basophils activated by TLR4 stimulation in type 1 AIP (9.875 ± 1.148%) and atopic dermatitis (11.768 ± 1.899%) were significantly higher than those in healthy subjects (5.051 ± 0.730%; P < 0.05). Levels of basophils activated by TLR2 stimulation were higher in seven type 1 AIP cases. Furthermore, stimulation of TLR2 and/or TLR4, which were expressed by basophils in pancreas, activated basophils in peripheral blood. CONCLUSIONS: Basophils activated via TLR signaling may play an important role in the pathophysiology of type 1 AIP.
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spelling pubmed-58472052018-03-20 Basophils activated via TLR signaling may contribute to pathophysiology of type 1 autoimmune pancreatitis Yanagawa, Masato Uchida, Kazushige Ando, Yugo Tomiyama, Takashi Yamaguchi, Takashi Ikeura, Tsukasa Fukui, Toshiro Nishio, Akiyoshi Uemura, Yoshiko Miyara, Takayuki Okamoto, Hiroyuki Satoi, Souhei Okazaki, Kazuichi J Gastroenterol Original Article—Liver, Pancreas, and Biliary Tract BACKGROUND: Pathophysiology of type 1 autoimmune pancreatitis (AIP) is still unclear. We previously reported that M2 macrophages might play an important role in type 1 AIP. Recently, it has been reported that basophils regulate differentiation to M2 macrophages. In this study, we investigated basophils from the pancreatic tissue and peripheral blood of individuals with type 1 AIP. METHODS: By using immunohistochemistry, we investigated basophils in pancreatic tissue from 13 patients with type 1 AIP and examined expression of toll-like receptors (TLRs) by these cells. Additionally, we obtained peripheral blood samples from 27 healthy subjects, 40 patients with type 1 AIP, 8 patients with alcoholic chronic pancreatitis, 10 patients with bronchial asthma, and 10 patients with atopic dermatitis, and analyzed activation of basophils by stimulating them with ligands of TLR1–9. We also compared TLR expression in basophils from the tissue and blood samples. RESULTS: Basophils were detected in pancreatic tissues from 10 of 13 patients with type 1 AIP. Flow cytometric analysis revealed that the ratios of basophils activated by TLR4 stimulation in type 1 AIP (9.875 ± 1.148%) and atopic dermatitis (11.768 ± 1.899%) were significantly higher than those in healthy subjects (5.051 ± 0.730%; P < 0.05). Levels of basophils activated by TLR2 stimulation were higher in seven type 1 AIP cases. Furthermore, stimulation of TLR2 and/or TLR4, which were expressed by basophils in pancreas, activated basophils in peripheral blood. CONCLUSIONS: Basophils activated via TLR signaling may play an important role in the pathophysiology of type 1 AIP. Springer Japan 2017-09-18 2018 /pmc/articles/PMC5847205/ /pubmed/28921377 http://dx.doi.org/10.1007/s00535-017-1390-6 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article—Liver, Pancreas, and Biliary Tract
Yanagawa, Masato
Uchida, Kazushige
Ando, Yugo
Tomiyama, Takashi
Yamaguchi, Takashi
Ikeura, Tsukasa
Fukui, Toshiro
Nishio, Akiyoshi
Uemura, Yoshiko
Miyara, Takayuki
Okamoto, Hiroyuki
Satoi, Souhei
Okazaki, Kazuichi
Basophils activated via TLR signaling may contribute to pathophysiology of type 1 autoimmune pancreatitis
title Basophils activated via TLR signaling may contribute to pathophysiology of type 1 autoimmune pancreatitis
title_full Basophils activated via TLR signaling may contribute to pathophysiology of type 1 autoimmune pancreatitis
title_fullStr Basophils activated via TLR signaling may contribute to pathophysiology of type 1 autoimmune pancreatitis
title_full_unstemmed Basophils activated via TLR signaling may contribute to pathophysiology of type 1 autoimmune pancreatitis
title_short Basophils activated via TLR signaling may contribute to pathophysiology of type 1 autoimmune pancreatitis
title_sort basophils activated via tlr signaling may contribute to pathophysiology of type 1 autoimmune pancreatitis
topic Original Article—Liver, Pancreas, and Biliary Tract
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847205/
https://www.ncbi.nlm.nih.gov/pubmed/28921377
http://dx.doi.org/10.1007/s00535-017-1390-6
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