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Distinguishing cell shoving mechanisms

Motivated by in vitro time–lapse images of ovarian cancer spheroids inducing mesothelial cell clearance, the traditional agent–based model of cell migration, based on simple volume exclusion, was extended to include the possibility that a cell seeking to move into an occupied location may push the r...

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Detalles Bibliográficos
Autores principales: Nan, Pingyu, Walsh, Darragh M., Landman, Kerry A., Hughes, Barry D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847236/
https://www.ncbi.nlm.nih.gov/pubmed/29529095
http://dx.doi.org/10.1371/journal.pone.0193975
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author Nan, Pingyu
Walsh, Darragh M.
Landman, Kerry A.
Hughes, Barry D.
author_facet Nan, Pingyu
Walsh, Darragh M.
Landman, Kerry A.
Hughes, Barry D.
author_sort Nan, Pingyu
collection PubMed
description Motivated by in vitro time–lapse images of ovarian cancer spheroids inducing mesothelial cell clearance, the traditional agent–based model of cell migration, based on simple volume exclusion, was extended to include the possibility that a cell seeking to move into an occupied location may push the resident cell, and any cells neighbouring it, out of the way to occupy that location. In traditional discrete models of motile cells with volume exclusion such a move would be aborted. We introduce a new shoving mechanism which allows cells to choose the direction to shove cells that expends the least amount of shoving effort (to account for the likely resistance of cells to being pushed). We call this motility rule ‘smart shoving’. We examine whether agent–based simulations of different shoving mechanisms can be distinguished on the basis of single realisations and averages over many realisations. We emphasise the difficulty in distinguishing cell mechanisms from cellular automata simulations based on snap–shots of cell distributions, site–occupancy averages and the evolution of the number of cells of each species averaged over many realisations. This difficulty suggests the need for higher resolution cell tracking.
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spelling pubmed-58472362018-03-23 Distinguishing cell shoving mechanisms Nan, Pingyu Walsh, Darragh M. Landman, Kerry A. Hughes, Barry D. PLoS One Research Article Motivated by in vitro time–lapse images of ovarian cancer spheroids inducing mesothelial cell clearance, the traditional agent–based model of cell migration, based on simple volume exclusion, was extended to include the possibility that a cell seeking to move into an occupied location may push the resident cell, and any cells neighbouring it, out of the way to occupy that location. In traditional discrete models of motile cells with volume exclusion such a move would be aborted. We introduce a new shoving mechanism which allows cells to choose the direction to shove cells that expends the least amount of shoving effort (to account for the likely resistance of cells to being pushed). We call this motility rule ‘smart shoving’. We examine whether agent–based simulations of different shoving mechanisms can be distinguished on the basis of single realisations and averages over many realisations. We emphasise the difficulty in distinguishing cell mechanisms from cellular automata simulations based on snap–shots of cell distributions, site–occupancy averages and the evolution of the number of cells of each species averaged over many realisations. This difficulty suggests the need for higher resolution cell tracking. Public Library of Science 2018-03-12 /pmc/articles/PMC5847236/ /pubmed/29529095 http://dx.doi.org/10.1371/journal.pone.0193975 Text en © 2018 Nan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Nan, Pingyu
Walsh, Darragh M.
Landman, Kerry A.
Hughes, Barry D.
Distinguishing cell shoving mechanisms
title Distinguishing cell shoving mechanisms
title_full Distinguishing cell shoving mechanisms
title_fullStr Distinguishing cell shoving mechanisms
title_full_unstemmed Distinguishing cell shoving mechanisms
title_short Distinguishing cell shoving mechanisms
title_sort distinguishing cell shoving mechanisms
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847236/
https://www.ncbi.nlm.nih.gov/pubmed/29529095
http://dx.doi.org/10.1371/journal.pone.0193975
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