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Distinguishing cell shoving mechanisms
Motivated by in vitro time–lapse images of ovarian cancer spheroids inducing mesothelial cell clearance, the traditional agent–based model of cell migration, based on simple volume exclusion, was extended to include the possibility that a cell seeking to move into an occupied location may push the r...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847236/ https://www.ncbi.nlm.nih.gov/pubmed/29529095 http://dx.doi.org/10.1371/journal.pone.0193975 |
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author | Nan, Pingyu Walsh, Darragh M. Landman, Kerry A. Hughes, Barry D. |
author_facet | Nan, Pingyu Walsh, Darragh M. Landman, Kerry A. Hughes, Barry D. |
author_sort | Nan, Pingyu |
collection | PubMed |
description | Motivated by in vitro time–lapse images of ovarian cancer spheroids inducing mesothelial cell clearance, the traditional agent–based model of cell migration, based on simple volume exclusion, was extended to include the possibility that a cell seeking to move into an occupied location may push the resident cell, and any cells neighbouring it, out of the way to occupy that location. In traditional discrete models of motile cells with volume exclusion such a move would be aborted. We introduce a new shoving mechanism which allows cells to choose the direction to shove cells that expends the least amount of shoving effort (to account for the likely resistance of cells to being pushed). We call this motility rule ‘smart shoving’. We examine whether agent–based simulations of different shoving mechanisms can be distinguished on the basis of single realisations and averages over many realisations. We emphasise the difficulty in distinguishing cell mechanisms from cellular automata simulations based on snap–shots of cell distributions, site–occupancy averages and the evolution of the number of cells of each species averaged over many realisations. This difficulty suggests the need for higher resolution cell tracking. |
format | Online Article Text |
id | pubmed-5847236 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58472362018-03-23 Distinguishing cell shoving mechanisms Nan, Pingyu Walsh, Darragh M. Landman, Kerry A. Hughes, Barry D. PLoS One Research Article Motivated by in vitro time–lapse images of ovarian cancer spheroids inducing mesothelial cell clearance, the traditional agent–based model of cell migration, based on simple volume exclusion, was extended to include the possibility that a cell seeking to move into an occupied location may push the resident cell, and any cells neighbouring it, out of the way to occupy that location. In traditional discrete models of motile cells with volume exclusion such a move would be aborted. We introduce a new shoving mechanism which allows cells to choose the direction to shove cells that expends the least amount of shoving effort (to account for the likely resistance of cells to being pushed). We call this motility rule ‘smart shoving’. We examine whether agent–based simulations of different shoving mechanisms can be distinguished on the basis of single realisations and averages over many realisations. We emphasise the difficulty in distinguishing cell mechanisms from cellular automata simulations based on snap–shots of cell distributions, site–occupancy averages and the evolution of the number of cells of each species averaged over many realisations. This difficulty suggests the need for higher resolution cell tracking. Public Library of Science 2018-03-12 /pmc/articles/PMC5847236/ /pubmed/29529095 http://dx.doi.org/10.1371/journal.pone.0193975 Text en © 2018 Nan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Nan, Pingyu Walsh, Darragh M. Landman, Kerry A. Hughes, Barry D. Distinguishing cell shoving mechanisms |
title | Distinguishing cell shoving mechanisms |
title_full | Distinguishing cell shoving mechanisms |
title_fullStr | Distinguishing cell shoving mechanisms |
title_full_unstemmed | Distinguishing cell shoving mechanisms |
title_short | Distinguishing cell shoving mechanisms |
title_sort | distinguishing cell shoving mechanisms |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847236/ https://www.ncbi.nlm.nih.gov/pubmed/29529095 http://dx.doi.org/10.1371/journal.pone.0193975 |
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