Building the atomic model of a boreal lake virus of unknown fold in a 3.9 Å cryo-EM map

We report here the protocol adopted to build the atomic model of the newly discovered virus FLiP (Flavobacterium infecting, lipid-containing phage) into 3.9 Å cryo-electron microscopy (cryo-EM) maps. In particular, this report discusses the combination of density modification procedures, automatic m...

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Detalles Bibliográficos
Autores principales: De Colibus, Luigi, Stuart, David I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847487/
https://www.ncbi.nlm.nih.gov/pubmed/29092773
http://dx.doi.org/10.1016/j.jsb.2017.10.010
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author De Colibus, Luigi
Stuart, David I.
author_facet De Colibus, Luigi
Stuart, David I.
author_sort De Colibus, Luigi
collection PubMed
description We report here the protocol adopted to build the atomic model of the newly discovered virus FLiP (Flavobacterium infecting, lipid-containing phage) into 3.9 Å cryo-electron microscopy (cryo-EM) maps. In particular, this report discusses the combination of density modification procedures, automatic model building and bioinformatics tools applied to guide the tracing of the major capsid protein (MCP) of this virus. The protocol outlined here may serve as a reference for future structural determination by cryo-EM of viruses lacking detectable structural homologues.
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spelling pubmed-58474872018-04-01 Building the atomic model of a boreal lake virus of unknown fold in a 3.9 Å cryo-EM map De Colibus, Luigi Stuart, David I. J Struct Biol Article We report here the protocol adopted to build the atomic model of the newly discovered virus FLiP (Flavobacterium infecting, lipid-containing phage) into 3.9 Å cryo-electron microscopy (cryo-EM) maps. In particular, this report discusses the combination of density modification procedures, automatic model building and bioinformatics tools applied to guide the tracing of the major capsid protein (MCP) of this virus. The protocol outlined here may serve as a reference for future structural determination by cryo-EM of viruses lacking detectable structural homologues. Academic Press 2018-04 /pmc/articles/PMC5847487/ /pubmed/29092773 http://dx.doi.org/10.1016/j.jsb.2017.10.010 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
De Colibus, Luigi
Stuart, David I.
Building the atomic model of a boreal lake virus of unknown fold in a 3.9 Å cryo-EM map
title Building the atomic model of a boreal lake virus of unknown fold in a 3.9 Å cryo-EM map
title_full Building the atomic model of a boreal lake virus of unknown fold in a 3.9 Å cryo-EM map
title_fullStr Building the atomic model of a boreal lake virus of unknown fold in a 3.9 Å cryo-EM map
title_full_unstemmed Building the atomic model of a boreal lake virus of unknown fold in a 3.9 Å cryo-EM map
title_short Building the atomic model of a boreal lake virus of unknown fold in a 3.9 Å cryo-EM map
title_sort building the atomic model of a boreal lake virus of unknown fold in a 3.9 å cryo-em map
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847487/
https://www.ncbi.nlm.nih.gov/pubmed/29092773
http://dx.doi.org/10.1016/j.jsb.2017.10.010
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