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In Vitro Study of the Cytotoxic, Cytostatic, and Antigenotoxic Profile of Hemidesmus indicus (L.) R.Br. (Apocynaceae) Crude Drug Extract on T Lymphoblastic Cells

In traditional Indian medicine, the crude drug Hemidesmus indicus root—commonly known as Indian sarsaparilla—is used alone or in poly-herbal preparations for the treatment of a wide range of diseases. The present study focuses on the cancer chemopreventive and therapeutic potential of H. indicus ext...

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Autores principales: Turrini, Eleonora, Calcabrini, Cinzia, Tacchini, Massimo, Efferth, Thomas, Sacchetti, Gianni, Guerrini, Alessandra, Paganetto, Guglielmo, Catanzaro, Elena, Greco, Giulia, Fimognari, Carmela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5848171/
https://www.ncbi.nlm.nih.gov/pubmed/29415441
http://dx.doi.org/10.3390/toxins10020070
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author Turrini, Eleonora
Calcabrini, Cinzia
Tacchini, Massimo
Efferth, Thomas
Sacchetti, Gianni
Guerrini, Alessandra
Paganetto, Guglielmo
Catanzaro, Elena
Greco, Giulia
Fimognari, Carmela
author_facet Turrini, Eleonora
Calcabrini, Cinzia
Tacchini, Massimo
Efferth, Thomas
Sacchetti, Gianni
Guerrini, Alessandra
Paganetto, Guglielmo
Catanzaro, Elena
Greco, Giulia
Fimognari, Carmela
author_sort Turrini, Eleonora
collection PubMed
description In traditional Indian medicine, the crude drug Hemidesmus indicus root—commonly known as Indian sarsaparilla—is used alone or in poly-herbal preparations for the treatment of a wide range of diseases. The present study focuses on the cancer chemopreventive and therapeutic potential of H. indicus extracts on an acute lymphoblastic leukemia cell line (CCRF-CEM). With this aim in mind, we subjected H. indicus roots to two subsequent extractions (hydro-alcoholic extraction and soxhlet extraction). As DNA damage is an important prerequisite for the induction of mutations/cancer by genotoxic carcinogens, cancer chemoprevention may be achieved by preventing genotoxicity. Through an integrated experimental approach, we explored the genoprotective potential of the soxhlet H. indicus extract against different mutagenic compounds and its cytotoxic, proapoptotic, and cytostatic properties. In our experimental conditions, H. indicus induced a cytotoxic effect involving the activation of both intrinsic and extrinsic apoptotic pathways and blocked the cell cycle in the S phase. Moreover, the antigenotoxicity results showed that the extract was able to mitigate DNA damage, an essential mechanism for its applicability as a chemopreventive agent, via either the modulation of extracellular and intracellular events involved in DNA damage. These data add to the growing body of evidence that H. indicus can represent a noteworthy strategy to target early and late stages of cancer.
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spelling pubmed-58481712018-03-14 In Vitro Study of the Cytotoxic, Cytostatic, and Antigenotoxic Profile of Hemidesmus indicus (L.) R.Br. (Apocynaceae) Crude Drug Extract on T Lymphoblastic Cells Turrini, Eleonora Calcabrini, Cinzia Tacchini, Massimo Efferth, Thomas Sacchetti, Gianni Guerrini, Alessandra Paganetto, Guglielmo Catanzaro, Elena Greco, Giulia Fimognari, Carmela Toxins (Basel) Article In traditional Indian medicine, the crude drug Hemidesmus indicus root—commonly known as Indian sarsaparilla—is used alone or in poly-herbal preparations for the treatment of a wide range of diseases. The present study focuses on the cancer chemopreventive and therapeutic potential of H. indicus extracts on an acute lymphoblastic leukemia cell line (CCRF-CEM). With this aim in mind, we subjected H. indicus roots to two subsequent extractions (hydro-alcoholic extraction and soxhlet extraction). As DNA damage is an important prerequisite for the induction of mutations/cancer by genotoxic carcinogens, cancer chemoprevention may be achieved by preventing genotoxicity. Through an integrated experimental approach, we explored the genoprotective potential of the soxhlet H. indicus extract against different mutagenic compounds and its cytotoxic, proapoptotic, and cytostatic properties. In our experimental conditions, H. indicus induced a cytotoxic effect involving the activation of both intrinsic and extrinsic apoptotic pathways and blocked the cell cycle in the S phase. Moreover, the antigenotoxicity results showed that the extract was able to mitigate DNA damage, an essential mechanism for its applicability as a chemopreventive agent, via either the modulation of extracellular and intracellular events involved in DNA damage. These data add to the growing body of evidence that H. indicus can represent a noteworthy strategy to target early and late stages of cancer. MDPI 2018-02-06 /pmc/articles/PMC5848171/ /pubmed/29415441 http://dx.doi.org/10.3390/toxins10020070 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Turrini, Eleonora
Calcabrini, Cinzia
Tacchini, Massimo
Efferth, Thomas
Sacchetti, Gianni
Guerrini, Alessandra
Paganetto, Guglielmo
Catanzaro, Elena
Greco, Giulia
Fimognari, Carmela
In Vitro Study of the Cytotoxic, Cytostatic, and Antigenotoxic Profile of Hemidesmus indicus (L.) R.Br. (Apocynaceae) Crude Drug Extract on T Lymphoblastic Cells
title In Vitro Study of the Cytotoxic, Cytostatic, and Antigenotoxic Profile of Hemidesmus indicus (L.) R.Br. (Apocynaceae) Crude Drug Extract on T Lymphoblastic Cells
title_full In Vitro Study of the Cytotoxic, Cytostatic, and Antigenotoxic Profile of Hemidesmus indicus (L.) R.Br. (Apocynaceae) Crude Drug Extract on T Lymphoblastic Cells
title_fullStr In Vitro Study of the Cytotoxic, Cytostatic, and Antigenotoxic Profile of Hemidesmus indicus (L.) R.Br. (Apocynaceae) Crude Drug Extract on T Lymphoblastic Cells
title_full_unstemmed In Vitro Study of the Cytotoxic, Cytostatic, and Antigenotoxic Profile of Hemidesmus indicus (L.) R.Br. (Apocynaceae) Crude Drug Extract on T Lymphoblastic Cells
title_short In Vitro Study of the Cytotoxic, Cytostatic, and Antigenotoxic Profile of Hemidesmus indicus (L.) R.Br. (Apocynaceae) Crude Drug Extract on T Lymphoblastic Cells
title_sort in vitro study of the cytotoxic, cytostatic, and antigenotoxic profile of hemidesmus indicus (l.) r.br. (apocynaceae) crude drug extract on t lymphoblastic cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5848171/
https://www.ncbi.nlm.nih.gov/pubmed/29415441
http://dx.doi.org/10.3390/toxins10020070
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