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Quantitative evaluation of canine urinary bladder transitional cell carcinoma using contrast-enhanced ultrasonography

BACKGROUND: In veterinary medicine, contrast-enhanced ultrasonography allowed the accurate quantification of liver, splenic and kidney vascularization in healthy dogs and the differentiation between malignant and benign hepatic, renal, and splenic nodules in dogs and cats based on perfusion patterns...

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Autores principales: Macrì, Francesco, Di Pietro, Simona, Mangano, Cyndi, Pugliese, Michela, Mazzullo, Giuseppe, Iannelli, Nicola M., Angileri, Vito, Morabito, Simona, De Majo, Massimo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5848439/
https://www.ncbi.nlm.nih.gov/pubmed/29530040
http://dx.doi.org/10.1186/s12917-018-1384-5
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author Macrì, Francesco
Di Pietro, Simona
Mangano, Cyndi
Pugliese, Michela
Mazzullo, Giuseppe
Iannelli, Nicola M.
Angileri, Vito
Morabito, Simona
De Majo, Massimo
author_facet Macrì, Francesco
Di Pietro, Simona
Mangano, Cyndi
Pugliese, Michela
Mazzullo, Giuseppe
Iannelli, Nicola M.
Angileri, Vito
Morabito, Simona
De Majo, Massimo
author_sort Macrì, Francesco
collection PubMed
description BACKGROUND: In veterinary medicine, contrast-enhanced ultrasonography allowed the accurate quantification of liver, splenic and kidney vascularization in healthy dogs and the differentiation between malignant and benign hepatic, renal, and splenic nodules in dogs and cats based on perfusion patterns. The utility of contrast-enhanced ultrasonography in other applications is still under study. The aim of this study was to develop diagnostic criteria by contrast-enhanced ultrasonography in 8 client-owned adult dogs affected by urinary bladder transitional cell carcinoma with definitive diagnosis made by cytopathologic evaluation after suction biopsy. The contrast enhancement pattern and the quantification of blood flow parameters of this tumor were reported. RESULTS: Examinations with B-mode, Doppler ultrasonography and contrast-enhanced ultrasonography were performed in all not sedated dogs. Assessments of bladder masses and bladder wall infiltration were performed. Each dog received 2 bolus injections of sulfur hexafluoride during the contrast-enhanced ultrasonography. Quantitative analysis of the contrast-enhanced ultrasonography images were performed. For each dog, one region of interest was manually drawn around the entire tumor. Software analysis of contrast-enhanced time-intensity curves was used to identify peak enhancement, time to peak enhancement, regional blood volume, regional blood flow, and mean transit time. Contrast-enhanced ultrasonography showed an avid enhancement of the tumour tissue, with a heterogeneous or homogeneous pattern. The exam also showed the loss of planes between the lesion and the muscular layer. The presence of vascularized tissue through the bladder wall confirms the infiltrative feature of the tumour. Post-processing quantitative analysis showed a time-intensity curve with a rapid wash-in, a low level of signal intensity and a slow wash-out. CONCLUSIONS: Contrast-enhanced ultrasonography provided useful clinical information and defined a vascular enhancement patterns and calculated parameters associated with TCC. It may be a useful, noninvasive and reproducible tool for detecting these tumors in dogs.
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spelling pubmed-58484392018-03-21 Quantitative evaluation of canine urinary bladder transitional cell carcinoma using contrast-enhanced ultrasonography Macrì, Francesco Di Pietro, Simona Mangano, Cyndi Pugliese, Michela Mazzullo, Giuseppe Iannelli, Nicola M. Angileri, Vito Morabito, Simona De Majo, Massimo BMC Vet Res Research Article BACKGROUND: In veterinary medicine, contrast-enhanced ultrasonography allowed the accurate quantification of liver, splenic and kidney vascularization in healthy dogs and the differentiation between malignant and benign hepatic, renal, and splenic nodules in dogs and cats based on perfusion patterns. The utility of contrast-enhanced ultrasonography in other applications is still under study. The aim of this study was to develop diagnostic criteria by contrast-enhanced ultrasonography in 8 client-owned adult dogs affected by urinary bladder transitional cell carcinoma with definitive diagnosis made by cytopathologic evaluation after suction biopsy. The contrast enhancement pattern and the quantification of blood flow parameters of this tumor were reported. RESULTS: Examinations with B-mode, Doppler ultrasonography and contrast-enhanced ultrasonography were performed in all not sedated dogs. Assessments of bladder masses and bladder wall infiltration were performed. Each dog received 2 bolus injections of sulfur hexafluoride during the contrast-enhanced ultrasonography. Quantitative analysis of the contrast-enhanced ultrasonography images were performed. For each dog, one region of interest was manually drawn around the entire tumor. Software analysis of contrast-enhanced time-intensity curves was used to identify peak enhancement, time to peak enhancement, regional blood volume, regional blood flow, and mean transit time. Contrast-enhanced ultrasonography showed an avid enhancement of the tumour tissue, with a heterogeneous or homogeneous pattern. The exam also showed the loss of planes between the lesion and the muscular layer. The presence of vascularized tissue through the bladder wall confirms the infiltrative feature of the tumour. Post-processing quantitative analysis showed a time-intensity curve with a rapid wash-in, a low level of signal intensity and a slow wash-out. CONCLUSIONS: Contrast-enhanced ultrasonography provided useful clinical information and defined a vascular enhancement patterns and calculated parameters associated with TCC. It may be a useful, noninvasive and reproducible tool for detecting these tumors in dogs. BioMed Central 2018-03-12 /pmc/articles/PMC5848439/ /pubmed/29530040 http://dx.doi.org/10.1186/s12917-018-1384-5 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Macrì, Francesco
Di Pietro, Simona
Mangano, Cyndi
Pugliese, Michela
Mazzullo, Giuseppe
Iannelli, Nicola M.
Angileri, Vito
Morabito, Simona
De Majo, Massimo
Quantitative evaluation of canine urinary bladder transitional cell carcinoma using contrast-enhanced ultrasonography
title Quantitative evaluation of canine urinary bladder transitional cell carcinoma using contrast-enhanced ultrasonography
title_full Quantitative evaluation of canine urinary bladder transitional cell carcinoma using contrast-enhanced ultrasonography
title_fullStr Quantitative evaluation of canine urinary bladder transitional cell carcinoma using contrast-enhanced ultrasonography
title_full_unstemmed Quantitative evaluation of canine urinary bladder transitional cell carcinoma using contrast-enhanced ultrasonography
title_short Quantitative evaluation of canine urinary bladder transitional cell carcinoma using contrast-enhanced ultrasonography
title_sort quantitative evaluation of canine urinary bladder transitional cell carcinoma using contrast-enhanced ultrasonography
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5848439/
https://www.ncbi.nlm.nih.gov/pubmed/29530040
http://dx.doi.org/10.1186/s12917-018-1384-5
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