Cargando…
Genetic diversity within dominant Enterocytozoon bieneusi genotypes in pre-weaned calves
BACKGROUND: Cattle are commonly infected with the microsporidian parasite Enterocytozoon bieneusi. Sequence characterization of E. bieneusi in these animals at the ribosomal internal transcribed spacer (ITS) locus had identified I, J and BEB4 as the dominant genotypes. However, current studies on E....
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5848593/ https://www.ncbi.nlm.nih.gov/pubmed/29530084 http://dx.doi.org/10.1186/s13071-018-2768-x |
_version_ | 1783305902067023872 |
---|---|
author | Tang, Chuanxiang Cai, Min Wang, Lin Guo, Yaqiong Li, Na Feng, Yaoyu Xiao, Lihua |
author_facet | Tang, Chuanxiang Cai, Min Wang, Lin Guo, Yaqiong Li, Na Feng, Yaoyu Xiao, Lihua |
author_sort | Tang, Chuanxiang |
collection | PubMed |
description | BACKGROUND: Cattle are commonly infected with the microsporidian parasite Enterocytozoon bieneusi. Sequence characterization of E. bieneusi in these animals at the ribosomal internal transcribed spacer (ITS) locus had identified I, J and BEB4 as the dominant genotypes. However, current studies on E. bieneusi in dairy cattle are mostly on infection rates and genotype distribution. This study aims to examine the intragenotypic diversity within dominant E. bieneusi genotypes in pre-weaned dairy calves in Shanghai, China. METHODS: Enterocytozoon bieneusi genotypes and subtypes were identified by PCR sequence analysis of ITS and multilocus sequence typing (MLST), based on material from farms. Chi-square test was used to examine differences in E. bieneusi infection rates between farms or age groups. RESULTS: The overall infection rate of E. bieneusi was 26.5% (214/809), ranging from 12.6% (Farm 5) to 38.5% (Farm 4). Infection rates increased with age during early life, with the peak infection rate (43.0%; 43/100) occurring at six weeks. Four genotypes were present, including J (n = 145, 67.8%), BEB4 (n = 59, 27.6%), CHN4 (n = 4, 1.9%) and CHN15 (n = 1, 0.5%), with the former two belonging to Group 2 and the latter two belonging to Group 1. Differences were detected in the distribution of the dominant genotypes J and BEB4 among five study farms. Altogether, 10 multilocus genotypes (MLGs) were identified in the two dominant ITS genotypes, including MLG-J1 to MLG-J8 of genotype J and MLG-B1 to MLG-B2 of genotype BEB4. MLG-B1 and MLG-B2 were recovered in Farms 1, 2 and 5, whereas MLG-J1 to MLG-J5 and MLG-J6 to MLG-J8 were found in Farms 3 and 4, respectively. CONCLUSIONS: There is extensive genetic heterogeneity within the dominant E. bieneusi genotypes J and BEB4 in dairy calves in Shanghai, China, and MLST should be used in molecular epidemiological studies of E. bieneusi in cattle. |
format | Online Article Text |
id | pubmed-5848593 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-58485932018-03-21 Genetic diversity within dominant Enterocytozoon bieneusi genotypes in pre-weaned calves Tang, Chuanxiang Cai, Min Wang, Lin Guo, Yaqiong Li, Na Feng, Yaoyu Xiao, Lihua Parasit Vectors Research BACKGROUND: Cattle are commonly infected with the microsporidian parasite Enterocytozoon bieneusi. Sequence characterization of E. bieneusi in these animals at the ribosomal internal transcribed spacer (ITS) locus had identified I, J and BEB4 as the dominant genotypes. However, current studies on E. bieneusi in dairy cattle are mostly on infection rates and genotype distribution. This study aims to examine the intragenotypic diversity within dominant E. bieneusi genotypes in pre-weaned dairy calves in Shanghai, China. METHODS: Enterocytozoon bieneusi genotypes and subtypes were identified by PCR sequence analysis of ITS and multilocus sequence typing (MLST), based on material from farms. Chi-square test was used to examine differences in E. bieneusi infection rates between farms or age groups. RESULTS: The overall infection rate of E. bieneusi was 26.5% (214/809), ranging from 12.6% (Farm 5) to 38.5% (Farm 4). Infection rates increased with age during early life, with the peak infection rate (43.0%; 43/100) occurring at six weeks. Four genotypes were present, including J (n = 145, 67.8%), BEB4 (n = 59, 27.6%), CHN4 (n = 4, 1.9%) and CHN15 (n = 1, 0.5%), with the former two belonging to Group 2 and the latter two belonging to Group 1. Differences were detected in the distribution of the dominant genotypes J and BEB4 among five study farms. Altogether, 10 multilocus genotypes (MLGs) were identified in the two dominant ITS genotypes, including MLG-J1 to MLG-J8 of genotype J and MLG-B1 to MLG-B2 of genotype BEB4. MLG-B1 and MLG-B2 were recovered in Farms 1, 2 and 5, whereas MLG-J1 to MLG-J5 and MLG-J6 to MLG-J8 were found in Farms 3 and 4, respectively. CONCLUSIONS: There is extensive genetic heterogeneity within the dominant E. bieneusi genotypes J and BEB4 in dairy calves in Shanghai, China, and MLST should be used in molecular epidemiological studies of E. bieneusi in cattle. BioMed Central 2018-03-12 /pmc/articles/PMC5848593/ /pubmed/29530084 http://dx.doi.org/10.1186/s13071-018-2768-x Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Tang, Chuanxiang Cai, Min Wang, Lin Guo, Yaqiong Li, Na Feng, Yaoyu Xiao, Lihua Genetic diversity within dominant Enterocytozoon bieneusi genotypes in pre-weaned calves |
title | Genetic diversity within dominant Enterocytozoon bieneusi genotypes in pre-weaned calves |
title_full | Genetic diversity within dominant Enterocytozoon bieneusi genotypes in pre-weaned calves |
title_fullStr | Genetic diversity within dominant Enterocytozoon bieneusi genotypes in pre-weaned calves |
title_full_unstemmed | Genetic diversity within dominant Enterocytozoon bieneusi genotypes in pre-weaned calves |
title_short | Genetic diversity within dominant Enterocytozoon bieneusi genotypes in pre-weaned calves |
title_sort | genetic diversity within dominant enterocytozoon bieneusi genotypes in pre-weaned calves |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5848593/ https://www.ncbi.nlm.nih.gov/pubmed/29530084 http://dx.doi.org/10.1186/s13071-018-2768-x |
work_keys_str_mv | AT tangchuanxiang geneticdiversitywithindominantenterocytozoonbieneusigenotypesinpreweanedcalves AT caimin geneticdiversitywithindominantenterocytozoonbieneusigenotypesinpreweanedcalves AT wanglin geneticdiversitywithindominantenterocytozoonbieneusigenotypesinpreweanedcalves AT guoyaqiong geneticdiversitywithindominantenterocytozoonbieneusigenotypesinpreweanedcalves AT lina geneticdiversitywithindominantenterocytozoonbieneusigenotypesinpreweanedcalves AT fengyaoyu geneticdiversitywithindominantenterocytozoonbieneusigenotypesinpreweanedcalves AT xiaolihua geneticdiversitywithindominantenterocytozoonbieneusigenotypesinpreweanedcalves |