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Influence of Poly(L-Lactic Acid) Nanofibers and BMP-2–Containing Poly(L-Lactic Acid) Nanofibers on Growth and Osteogenic Differentiation of Human Mesenchymal Stem Cells

The aim of this study was to characterize synthetic poly-(L-lactic acid) (PLLA) nanofibers concerning their ability to promote growth and osteogenic differentiation of stem cells in vitro, as well as to test their suitability as a carrier system for growth factors. Fiber matrices composed of PLLA or...

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Autores principales: Schofer, Markus D., Fuchs-Winkelmann, Susanne, Gräbedünkel, Christian, Wack, Christina, Dersch, Roland, Rudisile, Markus, Wendorff, Joachim H., Greiner, Andreas, Paletta, Jürgen R. J., Boudriot, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5848682/
https://www.ncbi.nlm.nih.gov/pubmed/19112539
http://dx.doi.org/10.1100/tsw.2008.163
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author Schofer, Markus D.
Fuchs-Winkelmann, Susanne
Gräbedünkel, Christian
Wack, Christina
Dersch, Roland
Rudisile, Markus
Wendorff, Joachim H.
Greiner, Andreas
Paletta, Jürgen R. J.
Boudriot, Ulrich
author_facet Schofer, Markus D.
Fuchs-Winkelmann, Susanne
Gräbedünkel, Christian
Wack, Christina
Dersch, Roland
Rudisile, Markus
Wendorff, Joachim H.
Greiner, Andreas
Paletta, Jürgen R. J.
Boudriot, Ulrich
author_sort Schofer, Markus D.
collection PubMed
description The aim of this study was to characterize synthetic poly-(L-lactic acid) (PLLA) nanofibers concerning their ability to promote growth and osteogenic differentiation of stem cells in vitro, as well as to test their suitability as a carrier system for growth factors. Fiber matrices composed of PLLA or BMP-2–incorporated PLLA were seeded with human mesenchymal stem cells and cultivated over a period of 22 days under growth and osteoinductive conditions, and analyzed during the course of culture, with respect to gene expression of alkaline phosphatase (ALP), osteocalcin (OC), and collagen I (COL-I). Furthermore, COL-I and OC deposition, as well as cell densities and proliferation, were analyzed using fluorescence microscopy. Although the presence of nanofibers diminished the dexamethasone-induced proliferation, there were no differences in cell densities or deposition of either COL-I or OC after 22 days of culture. The gene expression of ALP, OC, and COL-I decreased in the initial phase of cell cultivation on PLLA nanofibers as compared to cover slip control, but normalized during the course of cultivation. The initial down-regulation was not observed when BMP-2 was directly incorporated into PLLA nanofibers by electrospinning, indicating that growth factors like BMP-2 might survive the spinning process in a bioactive form.
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spelling pubmed-58486822018-04-17 Influence of Poly(L-Lactic Acid) Nanofibers and BMP-2–Containing Poly(L-Lactic Acid) Nanofibers on Growth and Osteogenic Differentiation of Human Mesenchymal Stem Cells Schofer, Markus D. Fuchs-Winkelmann, Susanne Gräbedünkel, Christian Wack, Christina Dersch, Roland Rudisile, Markus Wendorff, Joachim H. Greiner, Andreas Paletta, Jürgen R. J. Boudriot, Ulrich ScientificWorldJournal Research Article The aim of this study was to characterize synthetic poly-(L-lactic acid) (PLLA) nanofibers concerning their ability to promote growth and osteogenic differentiation of stem cells in vitro, as well as to test their suitability as a carrier system for growth factors. Fiber matrices composed of PLLA or BMP-2–incorporated PLLA were seeded with human mesenchymal stem cells and cultivated over a period of 22 days under growth and osteoinductive conditions, and analyzed during the course of culture, with respect to gene expression of alkaline phosphatase (ALP), osteocalcin (OC), and collagen I (COL-I). Furthermore, COL-I and OC deposition, as well as cell densities and proliferation, were analyzed using fluorescence microscopy. Although the presence of nanofibers diminished the dexamethasone-induced proliferation, there were no differences in cell densities or deposition of either COL-I or OC after 22 days of culture. The gene expression of ALP, OC, and COL-I decreased in the initial phase of cell cultivation on PLLA nanofibers as compared to cover slip control, but normalized during the course of cultivation. The initial down-regulation was not observed when BMP-2 was directly incorporated into PLLA nanofibers by electrospinning, indicating that growth factors like BMP-2 might survive the spinning process in a bioactive form. TheScientificWorldJOURNAL 2008-12-25 /pmc/articles/PMC5848682/ /pubmed/19112539 http://dx.doi.org/10.1100/tsw.2008.163 Text en Copyright © 2008 Markus D. Schofer et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Schofer, Markus D.
Fuchs-Winkelmann, Susanne
Gräbedünkel, Christian
Wack, Christina
Dersch, Roland
Rudisile, Markus
Wendorff, Joachim H.
Greiner, Andreas
Paletta, Jürgen R. J.
Boudriot, Ulrich
Influence of Poly(L-Lactic Acid) Nanofibers and BMP-2–Containing Poly(L-Lactic Acid) Nanofibers on Growth and Osteogenic Differentiation of Human Mesenchymal Stem Cells
title Influence of Poly(L-Lactic Acid) Nanofibers and BMP-2–Containing Poly(L-Lactic Acid) Nanofibers on Growth and Osteogenic Differentiation of Human Mesenchymal Stem Cells
title_full Influence of Poly(L-Lactic Acid) Nanofibers and BMP-2–Containing Poly(L-Lactic Acid) Nanofibers on Growth and Osteogenic Differentiation of Human Mesenchymal Stem Cells
title_fullStr Influence of Poly(L-Lactic Acid) Nanofibers and BMP-2–Containing Poly(L-Lactic Acid) Nanofibers on Growth and Osteogenic Differentiation of Human Mesenchymal Stem Cells
title_full_unstemmed Influence of Poly(L-Lactic Acid) Nanofibers and BMP-2–Containing Poly(L-Lactic Acid) Nanofibers on Growth and Osteogenic Differentiation of Human Mesenchymal Stem Cells
title_short Influence of Poly(L-Lactic Acid) Nanofibers and BMP-2–Containing Poly(L-Lactic Acid) Nanofibers on Growth and Osteogenic Differentiation of Human Mesenchymal Stem Cells
title_sort influence of poly(l-lactic acid) nanofibers and bmp-2–containing poly(l-lactic acid) nanofibers on growth and osteogenic differentiation of human mesenchymal stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5848682/
https://www.ncbi.nlm.nih.gov/pubmed/19112539
http://dx.doi.org/10.1100/tsw.2008.163
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