Detection of Heteromers Formed by Cannabinoid CB(1), Dopamine D(2), and Adenosine A(2A) G-Protein-Coupled Receptors by Combining Bimolecular Fluorescence Complementation and Bioluminescence Energy Transfer

Functional interactions in signaling occur between dopamine D(2) (D(2)R) and cannabinoid CB(1) (CB(1)R) receptors, between CB(1)R and adenosine A(2A) (A(2A)R) receptors, and between D(2)R and A(2A)R. Furthermore, direct molecular interactions have been reported for the pairs CB(1)R-D(2)R, A(2A)R-D(2...

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Detalles Bibliográficos
Autores principales: Navarro, Gemma, Carriba, Paulina, Gandí, Jorge, Ciruela, Francisco, Casadó, Vicent, Cortés, Antoni, Mallol, Josefa, Canela, Enric I., Lluis, Carmen, Franco, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2008
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5848735/
https://www.ncbi.nlm.nih.gov/pubmed/18956124
http://dx.doi.org/10.1100/tsw.2008.136
Descripción
Sumario:Functional interactions in signaling occur between dopamine D(2) (D(2)R) and cannabinoid CB(1) (CB(1)R) receptors, between CB(1)R and adenosine A(2A) (A(2A)R) receptors, and between D(2)R and A(2A)R. Furthermore, direct molecular interactions have been reported for the pairs CB(1)R-D(2)R, A(2A)R-D(2)R, and CB(1)R-A(2A)R. Here a combination of bimolecular fluorescence complementation and bioluminescence energy transfer techniques was used to identify the occurrence of D(2)R-CB(1)R-A(2A)R hetero-oligomers in living cells.

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