A Tentative Mechanism of Solubilization of Neuropathy Target Esterase from Chicken Embryo Brain by Phospholipase A(2)

The neuropathy target esterase is a membrane-bound enzyme linked to organophosphate-induced distal neuropathy. Here we report a tentative mechanism of its solubilization from chicken embryo brains by using phospholipase A(2). The enzyme was released from brain membranes after degradation of their st...

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Detalles Bibliográficos
Autor principal: Seifert, Josef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2008
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5848763/
https://www.ncbi.nlm.nih.gov/pubmed/18661050
http://dx.doi.org/10.1100/tsw.2008.51
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author Seifert, Josef
author_facet Seifert, Josef
author_sort Seifert, Josef
collection PubMed
description The neuropathy target esterase is a membrane-bound enzyme linked to organophosphate-induced distal neuropathy. Here we report a tentative mechanism of its solubilization from chicken embryo brains by using phospholipase A(2). The enzyme was released from brain membranes after degradation of their structural phospholipids initiated by phospholipase A(2). L-α-lysophosphatidylcholine, tested as a representative product of phospholipid hydrolysis, was identified as a new efficient detergent for solubilization of the neuropathy target esterase.
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spelling pubmed-58487632018-04-17 A Tentative Mechanism of Solubilization of Neuropathy Target Esterase from Chicken Embryo Brain by Phospholipase A(2) Seifert, Josef ScientificWorldJournal Short Communication The neuropathy target esterase is a membrane-bound enzyme linked to organophosphate-induced distal neuropathy. Here we report a tentative mechanism of its solubilization from chicken embryo brains by using phospholipase A(2). The enzyme was released from brain membranes after degradation of their structural phospholipids initiated by phospholipase A(2). L-α-lysophosphatidylcholine, tested as a representative product of phospholipid hydrolysis, was identified as a new efficient detergent for solubilization of the neuropathy target esterase. TheScientificWorldJOURNAL 2008-04-01 /pmc/articles/PMC5848763/ /pubmed/18661050 http://dx.doi.org/10.1100/tsw.2008.51 Text en Copyright © 2008 Josef Seifert. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Seifert, Josef
A Tentative Mechanism of Solubilization of Neuropathy Target Esterase from Chicken Embryo Brain by Phospholipase A(2)
title A Tentative Mechanism of Solubilization of Neuropathy Target Esterase from Chicken Embryo Brain by Phospholipase A(2)
title_full A Tentative Mechanism of Solubilization of Neuropathy Target Esterase from Chicken Embryo Brain by Phospholipase A(2)
title_fullStr A Tentative Mechanism of Solubilization of Neuropathy Target Esterase from Chicken Embryo Brain by Phospholipase A(2)
title_full_unstemmed A Tentative Mechanism of Solubilization of Neuropathy Target Esterase from Chicken Embryo Brain by Phospholipase A(2)
title_short A Tentative Mechanism of Solubilization of Neuropathy Target Esterase from Chicken Embryo Brain by Phospholipase A(2)
title_sort tentative mechanism of solubilization of neuropathy target esterase from chicken embryo brain by phospholipase a(2)
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5848763/
https://www.ncbi.nlm.nih.gov/pubmed/18661050
http://dx.doi.org/10.1100/tsw.2008.51
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