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miR-199a-5p inhibits proliferation and induces apoptosis in hemangioma cells through targeting HIF1A
MicroRNAs (miRNAs) exhibit a crucial role in the regulation of angiogenesis and tumor progression, of which miR-199a-5p (miR-199a) has been reported to function as a tumor suppressor in multiple malignancies. However, the precise mechanisms underlying miR-199a in hemangiomas (HAs) remain elusive. In...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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SAGE Publications
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5849215/ https://www.ncbi.nlm.nih.gov/pubmed/29268640 http://dx.doi.org/10.1177/0394632017749357 |
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author | Wang, Yang Dai, Yu-Xin Wang, Shu-Qing Qiu, Ming-Ke Quan, Zhi-Wei Liu, Ying-Bin Ou, Jing-Min |
author_facet | Wang, Yang Dai, Yu-Xin Wang, Shu-Qing Qiu, Ming-Ke Quan, Zhi-Wei Liu, Ying-Bin Ou, Jing-Min |
author_sort | Wang, Yang |
collection | PubMed |
description | MicroRNAs (miRNAs) exhibit a crucial role in the regulation of angiogenesis and tumor progression, of which miR-199a-5p (miR-199a) has been reported to function as a tumor suppressor in multiple malignancies. However, the precise mechanisms underlying miR-199a in hemangiomas (HAs) remain elusive. In this study, we found that miR-199a had low expression level, while proliferating cell nuclear antigen (PCNA) had high expression level in proliferating-phase HAs compared with the involuting-phase HAs and normal tissues. Spearman correlation analysis revealed the negative correlation of miR-199a with PCNA expression in proliferating-phase HAs. In vitro experiments showed that restoration of miR-199a suppressed cell proliferation capability and induced cell apoptosis in HA-derived endothelial cells (HDEC) and CRL-2586 EOMA cells, followed with decreased PCNA expression and increased cleaved caspase-3 expression, but miR-199a inhibitor reversed these effects. Furthermore, HIF1A was identified as a target of miR-199a and had negative correlation with miR-199a expression in proliferating-phase HAs. Overexpression of HIF1A attenuated the anti-proliferation effect of miR-199a mimic in HAs cells. Taken together, our findings demonstrate that miR-199a may inhibit proliferation and induce apoptosis in HAs cells via targeting HIF1A and provide a potential therapeutic target for HAs. |
format | Online Article Text |
id | pubmed-5849215 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-58492152018-12-20 miR-199a-5p inhibits proliferation and induces apoptosis in hemangioma cells through targeting HIF1A Wang, Yang Dai, Yu-Xin Wang, Shu-Qing Qiu, Ming-Ke Quan, Zhi-Wei Liu, Ying-Bin Ou, Jing-Min Int J Immunopathol Pharmacol Original Research Article MicroRNAs (miRNAs) exhibit a crucial role in the regulation of angiogenesis and tumor progression, of which miR-199a-5p (miR-199a) has been reported to function as a tumor suppressor in multiple malignancies. However, the precise mechanisms underlying miR-199a in hemangiomas (HAs) remain elusive. In this study, we found that miR-199a had low expression level, while proliferating cell nuclear antigen (PCNA) had high expression level in proliferating-phase HAs compared with the involuting-phase HAs and normal tissues. Spearman correlation analysis revealed the negative correlation of miR-199a with PCNA expression in proliferating-phase HAs. In vitro experiments showed that restoration of miR-199a suppressed cell proliferation capability and induced cell apoptosis in HA-derived endothelial cells (HDEC) and CRL-2586 EOMA cells, followed with decreased PCNA expression and increased cleaved caspase-3 expression, but miR-199a inhibitor reversed these effects. Furthermore, HIF1A was identified as a target of miR-199a and had negative correlation with miR-199a expression in proliferating-phase HAs. Overexpression of HIF1A attenuated the anti-proliferation effect of miR-199a mimic in HAs cells. Taken together, our findings demonstrate that miR-199a may inhibit proliferation and induce apoptosis in HAs cells via targeting HIF1A and provide a potential therapeutic target for HAs. SAGE Publications 2017-12-22 /pmc/articles/PMC5849215/ /pubmed/29268640 http://dx.doi.org/10.1177/0394632017749357 Text en © The Author(s) 2017 http://www.creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Research Article Wang, Yang Dai, Yu-Xin Wang, Shu-Qing Qiu, Ming-Ke Quan, Zhi-Wei Liu, Ying-Bin Ou, Jing-Min miR-199a-5p inhibits proliferation and induces apoptosis in hemangioma cells through targeting HIF1A |
title | miR-199a-5p inhibits proliferation and induces apoptosis in
hemangioma cells through targeting HIF1A |
title_full | miR-199a-5p inhibits proliferation and induces apoptosis in
hemangioma cells through targeting HIF1A |
title_fullStr | miR-199a-5p inhibits proliferation and induces apoptosis in
hemangioma cells through targeting HIF1A |
title_full_unstemmed | miR-199a-5p inhibits proliferation and induces apoptosis in
hemangioma cells through targeting HIF1A |
title_short | miR-199a-5p inhibits proliferation and induces apoptosis in
hemangioma cells through targeting HIF1A |
title_sort | mir-199a-5p inhibits proliferation and induces apoptosis in
hemangioma cells through targeting hif1a |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5849215/ https://www.ncbi.nlm.nih.gov/pubmed/29268640 http://dx.doi.org/10.1177/0394632017749357 |
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