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Tea polyphenols inhibit the proliferation, migration, and invasion of melanoma cells through the down-regulation of TLR4

Melanoma is the most common skin cancer and malignant melanoma which can cause skin cancer-related deaths. Toll-like receptor 4 (TLR4) had been reported to play an important role in melanoma, and tea polyphenol (TP) is regarded as an anticancer substance. However, the relationship between TP and TLR...

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Autores principales: Chen, Xianjin, Chang, Lili, Qu, Yan, Liang, Jinning, Jin, Waishu, Xia, Xiujuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5849249/
https://www.ncbi.nlm.nih.gov/pubmed/29359608
http://dx.doi.org/10.1177/0394632017739531
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author Chen, Xianjin
Chang, Lili
Qu, Yan
Liang, Jinning
Jin, Waishu
Xia, Xiujuan
author_facet Chen, Xianjin
Chang, Lili
Qu, Yan
Liang, Jinning
Jin, Waishu
Xia, Xiujuan
author_sort Chen, Xianjin
collection PubMed
description Melanoma is the most common skin cancer and malignant melanoma which can cause skin cancer-related deaths. Toll-like receptor 4 (TLR4) had been reported to play an important role in melanoma, and tea polyphenol (TP) is regarded as an anticancer substance. However, the relationship between TP and TLR4 in melanoma is not well explored. Therefore, our aim is to figure out how TP has an influence on melanoma. Melanoma cell lines (B16F10 and A375) were treated with TP and lipopolysaccharides (LPS). Western blot assay was used to examine TLR4 expression, and MTT assay was conducted to assess proliferation. Wound healing assay was conducted to evaluate the migration of melanoma cells, and transwell assay was used to examine the melanoma cells’ invasiveness. Besides, in vivo experiments were practiced for TP function in mice with melanoma cells. TP inhibited the proliferation, migration and invasion ability of melanoma cells, which displayed a dosage and time dependence. TLR4 was highly expressed in melanoma cells compared with normal skin cells. TP could suppress TLR4 expression both in normal melanomas and in stimulated melanomas by TLR4 agonist LPS. Suppressing TLR4 in melanomas could inhibit cell function (proliferation, migration, and invasion), and blocking the expression of 67LR could abolish TP function on TLR4. TP can inhibit melanoma (B16F10) growth in vivo.
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spelling pubmed-58492492018-11-05 Tea polyphenols inhibit the proliferation, migration, and invasion of melanoma cells through the down-regulation of TLR4 Chen, Xianjin Chang, Lili Qu, Yan Liang, Jinning Jin, Waishu Xia, Xiujuan Int J Immunopathol Pharmacol Original Research Article Melanoma is the most common skin cancer and malignant melanoma which can cause skin cancer-related deaths. Toll-like receptor 4 (TLR4) had been reported to play an important role in melanoma, and tea polyphenol (TP) is regarded as an anticancer substance. However, the relationship between TP and TLR4 in melanoma is not well explored. Therefore, our aim is to figure out how TP has an influence on melanoma. Melanoma cell lines (B16F10 and A375) were treated with TP and lipopolysaccharides (LPS). Western blot assay was used to examine TLR4 expression, and MTT assay was conducted to assess proliferation. Wound healing assay was conducted to evaluate the migration of melanoma cells, and transwell assay was used to examine the melanoma cells’ invasiveness. Besides, in vivo experiments were practiced for TP function in mice with melanoma cells. TP inhibited the proliferation, migration and invasion ability of melanoma cells, which displayed a dosage and time dependence. TLR4 was highly expressed in melanoma cells compared with normal skin cells. TP could suppress TLR4 expression both in normal melanomas and in stimulated melanomas by TLR4 agonist LPS. Suppressing TLR4 in melanomas could inhibit cell function (proliferation, migration, and invasion), and blocking the expression of 67LR could abolish TP function on TLR4. TP can inhibit melanoma (B16F10) growth in vivo. SAGE Publications 2018-01-23 /pmc/articles/PMC5849249/ /pubmed/29359608 http://dx.doi.org/10.1177/0394632017739531 Text en © The Author(s) 2018 http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution 4.0 License (http://www.creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Research Article
Chen, Xianjin
Chang, Lili
Qu, Yan
Liang, Jinning
Jin, Waishu
Xia, Xiujuan
Tea polyphenols inhibit the proliferation, migration, and invasion of melanoma cells through the down-regulation of TLR4
title Tea polyphenols inhibit the proliferation, migration, and invasion of melanoma cells through the down-regulation of TLR4
title_full Tea polyphenols inhibit the proliferation, migration, and invasion of melanoma cells through the down-regulation of TLR4
title_fullStr Tea polyphenols inhibit the proliferation, migration, and invasion of melanoma cells through the down-regulation of TLR4
title_full_unstemmed Tea polyphenols inhibit the proliferation, migration, and invasion of melanoma cells through the down-regulation of TLR4
title_short Tea polyphenols inhibit the proliferation, migration, and invasion of melanoma cells through the down-regulation of TLR4
title_sort tea polyphenols inhibit the proliferation, migration, and invasion of melanoma cells through the down-regulation of tlr4
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5849249/
https://www.ncbi.nlm.nih.gov/pubmed/29359608
http://dx.doi.org/10.1177/0394632017739531
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