Cargando…
MicroRNA Signatures for circulating CD133-positive cells in hepatocellular carcinoma with HCV infection
AIM: Molecular characterization of the CD133+ stem cells associated with hepatocarinogensis through identifying the expression patterns of specific microRNAs (miRNAs). METHODS: We investigated the expression pattern of 13 miRNAs in purified CD133+ cells separated from the peripheral blood of healthy...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5849309/ https://www.ncbi.nlm.nih.gov/pubmed/29534065 http://dx.doi.org/10.1371/journal.pone.0193709 |
_version_ | 1783306029091520512 |
---|---|
author | Zekri, Abdel-Rahman N. El-Sisi, Enas Reda Youssef, Amira Salah El-Din Kamel, Mahmoud M. Nassar, Auhood Ahmed, Ola Sayed El Kassas, Mohamed Barakat, Ahmed Barakat Abd El-Motaleb, Alaa Ismail Bahnassy, Abeer A. |
author_facet | Zekri, Abdel-Rahman N. El-Sisi, Enas Reda Youssef, Amira Salah El-Din Kamel, Mahmoud M. Nassar, Auhood Ahmed, Ola Sayed El Kassas, Mohamed Barakat, Ahmed Barakat Abd El-Motaleb, Alaa Ismail Bahnassy, Abeer A. |
author_sort | Zekri, Abdel-Rahman N. |
collection | PubMed |
description | AIM: Molecular characterization of the CD133+ stem cells associated with hepatocarinogensis through identifying the expression patterns of specific microRNAs (miRNAs). METHODS: We investigated the expression pattern of 13 miRNAs in purified CD133+ cells separated from the peripheral blood of healthy volunteers, chronic hepatitis C (CHC), liver cirrhosis (LC) and hepatocellular carcinoma (HCC) patients a long with bone marrow samples from the healthy volunteers and the LC patients using custom miScript miRNA PCR array. RESULTS: The differential expression of the 13 studied miRNAs in CD133+ cells separated from the HCC patients' peripheral blood compared to the controls revealed that miR-602, miR-181b, miR-101, miR-122, miR-192, miR-125a-5p, and miR-221 were significantly up regulated (fold change = 1.8, 1.7, 2, 5.4, 1.6, 2.9 & 1.5 P value = 0.039, 0.0019, 0.0013, 0.0370, 00024, 0.000044 &0.000007 respectively). As for the HCC group compared to the CHC group; miR-602, miR-122, miR-181b, miR-125a-5p, and miR-192 were significantly up regulated (fold change = 13, 3.1, 2.8, 1.6 & 1.56, P value = 0.01, 0.001, 0.000004, 0.002 & 0.007 respectively). Upon comparing the HCC group to the LC group; miR-199a-3p, miR-192, miR-122, miR-181b, miR-224, miR-125a-5p, and miR-885-5p were significantly up regulated (fold change = 5, 6.7, 2.3, 3, 2.5, 4.2 & 39.5 P value = 0.001025, 0.000024, 0.000472, 0.000278, 0.000004, 0.000075 & 0.0000001 respectively) whereas miR-22 was significantly down regulated (fold change = 0.57 P value = 0.00002). Only, miR-192, miR-122, miR-181b and miR-125a-5p were significant common miRNAs in CD133+ cells of the HCC group compared to the other non-malignant groups. CONCLUSION: We identified a miRNA panel comprised of four miRNAs (miR-192, miR-122, miR-181b and miR-125a-5p) that may serve as a molecular tool for characterization of the CD133+ cells associated with different stages of hepatocarinogensis. This panel may aid in developing a new target therapy specific for those CD133+ cells. |
format | Online Article Text |
id | pubmed-5849309 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58493092018-03-23 MicroRNA Signatures for circulating CD133-positive cells in hepatocellular carcinoma with HCV infection Zekri, Abdel-Rahman N. El-Sisi, Enas Reda Youssef, Amira Salah El-Din Kamel, Mahmoud M. Nassar, Auhood Ahmed, Ola Sayed El Kassas, Mohamed Barakat, Ahmed Barakat Abd El-Motaleb, Alaa Ismail Bahnassy, Abeer A. PLoS One Research Article AIM: Molecular characterization of the CD133+ stem cells associated with hepatocarinogensis through identifying the expression patterns of specific microRNAs (miRNAs). METHODS: We investigated the expression pattern of 13 miRNAs in purified CD133+ cells separated from the peripheral blood of healthy volunteers, chronic hepatitis C (CHC), liver cirrhosis (LC) and hepatocellular carcinoma (HCC) patients a long with bone marrow samples from the healthy volunteers and the LC patients using custom miScript miRNA PCR array. RESULTS: The differential expression of the 13 studied miRNAs in CD133+ cells separated from the HCC patients' peripheral blood compared to the controls revealed that miR-602, miR-181b, miR-101, miR-122, miR-192, miR-125a-5p, and miR-221 were significantly up regulated (fold change = 1.8, 1.7, 2, 5.4, 1.6, 2.9 & 1.5 P value = 0.039, 0.0019, 0.0013, 0.0370, 00024, 0.000044 &0.000007 respectively). As for the HCC group compared to the CHC group; miR-602, miR-122, miR-181b, miR-125a-5p, and miR-192 were significantly up regulated (fold change = 13, 3.1, 2.8, 1.6 & 1.56, P value = 0.01, 0.001, 0.000004, 0.002 & 0.007 respectively). Upon comparing the HCC group to the LC group; miR-199a-3p, miR-192, miR-122, miR-181b, miR-224, miR-125a-5p, and miR-885-5p were significantly up regulated (fold change = 5, 6.7, 2.3, 3, 2.5, 4.2 & 39.5 P value = 0.001025, 0.000024, 0.000472, 0.000278, 0.000004, 0.000075 & 0.0000001 respectively) whereas miR-22 was significantly down regulated (fold change = 0.57 P value = 0.00002). Only, miR-192, miR-122, miR-181b and miR-125a-5p were significant common miRNAs in CD133+ cells of the HCC group compared to the other non-malignant groups. CONCLUSION: We identified a miRNA panel comprised of four miRNAs (miR-192, miR-122, miR-181b and miR-125a-5p) that may serve as a molecular tool for characterization of the CD133+ cells associated with different stages of hepatocarinogensis. This panel may aid in developing a new target therapy specific for those CD133+ cells. Public Library of Science 2018-03-13 /pmc/articles/PMC5849309/ /pubmed/29534065 http://dx.doi.org/10.1371/journal.pone.0193709 Text en © 2018 Zekri et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zekri, Abdel-Rahman N. El-Sisi, Enas Reda Youssef, Amira Salah El-Din Kamel, Mahmoud M. Nassar, Auhood Ahmed, Ola Sayed El Kassas, Mohamed Barakat, Ahmed Barakat Abd El-Motaleb, Alaa Ismail Bahnassy, Abeer A. MicroRNA Signatures for circulating CD133-positive cells in hepatocellular carcinoma with HCV infection |
title | MicroRNA Signatures for circulating CD133-positive cells in hepatocellular carcinoma with HCV infection |
title_full | MicroRNA Signatures for circulating CD133-positive cells in hepatocellular carcinoma with HCV infection |
title_fullStr | MicroRNA Signatures for circulating CD133-positive cells in hepatocellular carcinoma with HCV infection |
title_full_unstemmed | MicroRNA Signatures for circulating CD133-positive cells in hepatocellular carcinoma with HCV infection |
title_short | MicroRNA Signatures for circulating CD133-positive cells in hepatocellular carcinoma with HCV infection |
title_sort | microrna signatures for circulating cd133-positive cells in hepatocellular carcinoma with hcv infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5849309/ https://www.ncbi.nlm.nih.gov/pubmed/29534065 http://dx.doi.org/10.1371/journal.pone.0193709 |
work_keys_str_mv | AT zekriabdelrahmann micrornasignaturesforcirculatingcd133positivecellsinhepatocellularcarcinomawithhcvinfection AT elsisienasreda micrornasignaturesforcirculatingcd133positivecellsinhepatocellularcarcinomawithhcvinfection AT youssefamirasalaheldin micrornasignaturesforcirculatingcd133positivecellsinhepatocellularcarcinomawithhcvinfection AT kamelmahmoudm micrornasignaturesforcirculatingcd133positivecellsinhepatocellularcarcinomawithhcvinfection AT nassarauhood micrornasignaturesforcirculatingcd133positivecellsinhepatocellularcarcinomawithhcvinfection AT ahmedolasayed micrornasignaturesforcirculatingcd133positivecellsinhepatocellularcarcinomawithhcvinfection AT elkassasmohamed micrornasignaturesforcirculatingcd133positivecellsinhepatocellularcarcinomawithhcvinfection AT barakatahmedbarakat micrornasignaturesforcirculatingcd133positivecellsinhepatocellularcarcinomawithhcvinfection AT abdelmotalebalaaismail micrornasignaturesforcirculatingcd133positivecellsinhepatocellularcarcinomawithhcvinfection AT bahnassyabeera micrornasignaturesforcirculatingcd133positivecellsinhepatocellularcarcinomawithhcvinfection |