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Regulation of FSH expression by differentially expressed miR-186-5p in rat anterior adenohypophyseal cells
Follicle-stimulating hormone (FSH) has key roles in animal reproduction, including spermatogenesis and ovarian maturation. Many factors influence FSH secretion. However, despite the broad functions of microRNAs (miRNAs) via the regulation of target genes, little is known about their roles in FSH sec...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5849326/ https://www.ncbi.nlm.nih.gov/pubmed/29534107 http://dx.doi.org/10.1371/journal.pone.0194300 |
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author | Han, Dong-Xu Xiao, Yue Wang, Chang-Jiang Jiang, Hao Gao, Yan Yuan, Bao Zhang, Jia-Bao |
author_facet | Han, Dong-Xu Xiao, Yue Wang, Chang-Jiang Jiang, Hao Gao, Yan Yuan, Bao Zhang, Jia-Bao |
author_sort | Han, Dong-Xu |
collection | PubMed |
description | Follicle-stimulating hormone (FSH) has key roles in animal reproduction, including spermatogenesis and ovarian maturation. Many factors influence FSH secretion. However, despite the broad functions of microRNAs (miRNAs) via the regulation of target genes, little is known about their roles in FSH secretion. Our previous results suggested that miR-186-5p targets the 3′ UTR of FSHb; therefore, we examined whether miR-186-5p could regulate FSH secretion in rat anterior adenohypophyseal cells. miR-186-5p was transfected into rat anterior pituitary cells. The expression of FSHb and the secretion of FSH were examined by RT-qPCR and ELISA. A miR-186-5p mimic decreased the expression of FSHb compared with expression in the control group and decreased FSH secretion. In contrast, both the mRNA levels and secretion of FSH increased in response to miR-186-5p inhibitors. Our results demonstrate that miR-186-5p regulates FSH secretion by directly targeting the FSHb 3′ UTR, providing additional functional evidence for the importance of miRNAs in the regulation of animal reproduction. |
format | Online Article Text |
id | pubmed-5849326 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58493262018-03-23 Regulation of FSH expression by differentially expressed miR-186-5p in rat anterior adenohypophyseal cells Han, Dong-Xu Xiao, Yue Wang, Chang-Jiang Jiang, Hao Gao, Yan Yuan, Bao Zhang, Jia-Bao PLoS One Research Article Follicle-stimulating hormone (FSH) has key roles in animal reproduction, including spermatogenesis and ovarian maturation. Many factors influence FSH secretion. However, despite the broad functions of microRNAs (miRNAs) via the regulation of target genes, little is known about their roles in FSH secretion. Our previous results suggested that miR-186-5p targets the 3′ UTR of FSHb; therefore, we examined whether miR-186-5p could regulate FSH secretion in rat anterior adenohypophyseal cells. miR-186-5p was transfected into rat anterior pituitary cells. The expression of FSHb and the secretion of FSH were examined by RT-qPCR and ELISA. A miR-186-5p mimic decreased the expression of FSHb compared with expression in the control group and decreased FSH secretion. In contrast, both the mRNA levels and secretion of FSH increased in response to miR-186-5p inhibitors. Our results demonstrate that miR-186-5p regulates FSH secretion by directly targeting the FSHb 3′ UTR, providing additional functional evidence for the importance of miRNAs in the regulation of animal reproduction. Public Library of Science 2018-03-13 /pmc/articles/PMC5849326/ /pubmed/29534107 http://dx.doi.org/10.1371/journal.pone.0194300 Text en © 2018 Han et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Han, Dong-Xu Xiao, Yue Wang, Chang-Jiang Jiang, Hao Gao, Yan Yuan, Bao Zhang, Jia-Bao Regulation of FSH expression by differentially expressed miR-186-5p in rat anterior adenohypophyseal cells |
title | Regulation of FSH expression by differentially expressed miR-186-5p in rat anterior adenohypophyseal cells |
title_full | Regulation of FSH expression by differentially expressed miR-186-5p in rat anterior adenohypophyseal cells |
title_fullStr | Regulation of FSH expression by differentially expressed miR-186-5p in rat anterior adenohypophyseal cells |
title_full_unstemmed | Regulation of FSH expression by differentially expressed miR-186-5p in rat anterior adenohypophyseal cells |
title_short | Regulation of FSH expression by differentially expressed miR-186-5p in rat anterior adenohypophyseal cells |
title_sort | regulation of fsh expression by differentially expressed mir-186-5p in rat anterior adenohypophyseal cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5849326/ https://www.ncbi.nlm.nih.gov/pubmed/29534107 http://dx.doi.org/10.1371/journal.pone.0194300 |
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