Cargando…
Freely suspended perforated polymer nanomembranes for protein separations
Selective removal of nanometer-sized compounds such as proteins from fluids is an often challenging task in many scientific and industrial areas. Addressing such tasks with highly efficient and selective membranes is desirable since commonly used chromatographic approaches are expensive and difficul...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5849607/ https://www.ncbi.nlm.nih.gov/pubmed/29535317 http://dx.doi.org/10.1038/s41598-018-22200-4 |
_version_ | 1783306066160779264 |
---|---|
author | Schuster, Christian Rodler, Agnes Tscheliessnig, Rupert Jungbauer, Alois |
author_facet | Schuster, Christian Rodler, Agnes Tscheliessnig, Rupert Jungbauer, Alois |
author_sort | Schuster, Christian |
collection | PubMed |
description | Selective removal of nanometer-sized compounds such as proteins from fluids is an often challenging task in many scientific and industrial areas. Addressing such tasks with highly efficient and selective membranes is desirable since commonly used chromatographic approaches are expensive and difficult to scale up. Nanomembranes, molecularly thin separation layers, have been predicted and shown to possess outstanding properties but in spite ultra-fast diffusion times and high-resolution separation, to date they generally lack either of two crucial characteristics: compatibility with biological fluids and low-cost production. Here we report the fast and easy fabrication of highly crosslinked polymer membranes based on a thermoset resin (poly[(o-cresyl glycidyl ether)-co-formaldehyde (PCGF) cured with branched polyethyleneimine (PEI)) with nanoscale perforations of 25 nm diameter. During spin casting, microphase separation of a polylactide-co-glycolide induces the formation of nanometer sized domains that serve as templates for perforations which penetrate the 80 nm thick membranes. Ultrathin perforated nanomembranes can be freely suspended on the cm scale, exhibit high mechanical strength, low surface energies and a sharp permeability cutoff at a hydrodynamic diameter of 10 nm suitable for protein separations. |
format | Online Article Text |
id | pubmed-5849607 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58496072018-03-21 Freely suspended perforated polymer nanomembranes for protein separations Schuster, Christian Rodler, Agnes Tscheliessnig, Rupert Jungbauer, Alois Sci Rep Article Selective removal of nanometer-sized compounds such as proteins from fluids is an often challenging task in many scientific and industrial areas. Addressing such tasks with highly efficient and selective membranes is desirable since commonly used chromatographic approaches are expensive and difficult to scale up. Nanomembranes, molecularly thin separation layers, have been predicted and shown to possess outstanding properties but in spite ultra-fast diffusion times and high-resolution separation, to date they generally lack either of two crucial characteristics: compatibility with biological fluids and low-cost production. Here we report the fast and easy fabrication of highly crosslinked polymer membranes based on a thermoset resin (poly[(o-cresyl glycidyl ether)-co-formaldehyde (PCGF) cured with branched polyethyleneimine (PEI)) with nanoscale perforations of 25 nm diameter. During spin casting, microphase separation of a polylactide-co-glycolide induces the formation of nanometer sized domains that serve as templates for perforations which penetrate the 80 nm thick membranes. Ultrathin perforated nanomembranes can be freely suspended on the cm scale, exhibit high mechanical strength, low surface energies and a sharp permeability cutoff at a hydrodynamic diameter of 10 nm suitable for protein separations. Nature Publishing Group UK 2018-03-13 /pmc/articles/PMC5849607/ /pubmed/29535317 http://dx.doi.org/10.1038/s41598-018-22200-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Schuster, Christian Rodler, Agnes Tscheliessnig, Rupert Jungbauer, Alois Freely suspended perforated polymer nanomembranes for protein separations |
title | Freely suspended perforated polymer nanomembranes for protein separations |
title_full | Freely suspended perforated polymer nanomembranes for protein separations |
title_fullStr | Freely suspended perforated polymer nanomembranes for protein separations |
title_full_unstemmed | Freely suspended perforated polymer nanomembranes for protein separations |
title_short | Freely suspended perforated polymer nanomembranes for protein separations |
title_sort | freely suspended perforated polymer nanomembranes for protein separations |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5849607/ https://www.ncbi.nlm.nih.gov/pubmed/29535317 http://dx.doi.org/10.1038/s41598-018-22200-4 |
work_keys_str_mv | AT schusterchristian freelysuspendedperforatedpolymernanomembranesforproteinseparations AT rodleragnes freelysuspendedperforatedpolymernanomembranesforproteinseparations AT tscheliessnigrupert freelysuspendedperforatedpolymernanomembranesforproteinseparations AT jungbaueralois freelysuspendedperforatedpolymernanomembranesforproteinseparations |