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Highly efficient heritable targeted deletions of gene clusters and non-coding regulatory regions in Arabidopsis using CRISPR/Cas9

Genome editing using CRISPR/Cas9 is considered the best instrument for genome engineering in plants. This methodology is based on the nuclease activity of Cas9 that is guided to specific genome sequences by single guide RNAs (sgRNAs) thus enabling researchers to engineer simple mutations or large ch...

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Detalles Bibliográficos
Autores principales:	Durr, Julius, Papareddy, Ranjith, Nakajima, Keiji, Gutierrez-Marcos, Jose
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5849686/ https://www.ncbi.nlm.nih.gov/pubmed/29535386 http://dx.doi.org/10.1038/s41598-018-22667-1

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