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Transcriptome profiling of Issatchenkia orientalis under ethanol stress
Issatchenkia orientalis, a non-Saccharomyces yeast that can resist a wide variety of environmental stresses, has potential use in winemaking and bioethanol production. Little is known about gene expression or the physiology of I. orientalis under ethanol stress. In this study, high-throughput RNA se...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5849708/ https://www.ncbi.nlm.nih.gov/pubmed/29536208 http://dx.doi.org/10.1186/s13568-018-0568-5 |
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author | Miao, Yingjie Xiong, Guotong Li, Ruoyun Wu, Zufang Zhang, Xin Weng, Peifang |
author_facet | Miao, Yingjie Xiong, Guotong Li, Ruoyun Wu, Zufang Zhang, Xin Weng, Peifang |
author_sort | Miao, Yingjie |
collection | PubMed |
description | Issatchenkia orientalis, a non-Saccharomyces yeast that can resist a wide variety of environmental stresses, has potential use in winemaking and bioethanol production. Little is known about gene expression or the physiology of I. orientalis under ethanol stress. In this study, high-throughput RNA sequencing was used to investigate the transcriptome profile of I. orientalis in response to ethanol. 502 gene transcripts were differentially expressed, of which 451 were more abundant, and 51 less abundant, in cells subjected to 4 h of ethanol stress (10% v/v). Annotation and statistical analyses suggest that multiple genes involved in ergosterol biosynthesis, trehalose metabolism, and stress response are differentially expressed under these conditions. The up-regulation of molecular chaperones HSP90 and HSP70, and also genes associated with the ubiquitin–proteasome proteolytic pathway suggests that ethanol stress may cause aggregation of misfolded proteins. Finally, ethanol stress in I. orientalis appears to have a nitrogen starvation effect, and many genes involved in nutrient uptake were up-regulated. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13568-018-0568-5) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5849708 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-58497082018-03-21 Transcriptome profiling of Issatchenkia orientalis under ethanol stress Miao, Yingjie Xiong, Guotong Li, Ruoyun Wu, Zufang Zhang, Xin Weng, Peifang AMB Express Original Article Issatchenkia orientalis, a non-Saccharomyces yeast that can resist a wide variety of environmental stresses, has potential use in winemaking and bioethanol production. Little is known about gene expression or the physiology of I. orientalis under ethanol stress. In this study, high-throughput RNA sequencing was used to investigate the transcriptome profile of I. orientalis in response to ethanol. 502 gene transcripts were differentially expressed, of which 451 were more abundant, and 51 less abundant, in cells subjected to 4 h of ethanol stress (10% v/v). Annotation and statistical analyses suggest that multiple genes involved in ergosterol biosynthesis, trehalose metabolism, and stress response are differentially expressed under these conditions. The up-regulation of molecular chaperones HSP90 and HSP70, and also genes associated with the ubiquitin–proteasome proteolytic pathway suggests that ethanol stress may cause aggregation of misfolded proteins. Finally, ethanol stress in I. orientalis appears to have a nitrogen starvation effect, and many genes involved in nutrient uptake were up-regulated. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13568-018-0568-5) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2018-03-13 /pmc/articles/PMC5849708/ /pubmed/29536208 http://dx.doi.org/10.1186/s13568-018-0568-5 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Miao, Yingjie Xiong, Guotong Li, Ruoyun Wu, Zufang Zhang, Xin Weng, Peifang Transcriptome profiling of Issatchenkia orientalis under ethanol stress |
title | Transcriptome profiling of Issatchenkia orientalis under ethanol stress |
title_full | Transcriptome profiling of Issatchenkia orientalis under ethanol stress |
title_fullStr | Transcriptome profiling of Issatchenkia orientalis under ethanol stress |
title_full_unstemmed | Transcriptome profiling of Issatchenkia orientalis under ethanol stress |
title_short | Transcriptome profiling of Issatchenkia orientalis under ethanol stress |
title_sort | transcriptome profiling of issatchenkia orientalis under ethanol stress |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5849708/ https://www.ncbi.nlm.nih.gov/pubmed/29536208 http://dx.doi.org/10.1186/s13568-018-0568-5 |
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