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Transcriptome profiling of Issatchenkia orientalis under ethanol stress

Issatchenkia orientalis, a non-Saccharomyces yeast that can resist a wide variety of environmental stresses, has potential use in winemaking and bioethanol production. Little is known about gene expression or the physiology of I. orientalis under ethanol stress. In this study, high-throughput RNA se...

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Autores principales: Miao, Yingjie, Xiong, Guotong, Li, Ruoyun, Wu, Zufang, Zhang, Xin, Weng, Peifang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5849708/
https://www.ncbi.nlm.nih.gov/pubmed/29536208
http://dx.doi.org/10.1186/s13568-018-0568-5
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author Miao, Yingjie
Xiong, Guotong
Li, Ruoyun
Wu, Zufang
Zhang, Xin
Weng, Peifang
author_facet Miao, Yingjie
Xiong, Guotong
Li, Ruoyun
Wu, Zufang
Zhang, Xin
Weng, Peifang
author_sort Miao, Yingjie
collection PubMed
description Issatchenkia orientalis, a non-Saccharomyces yeast that can resist a wide variety of environmental stresses, has potential use in winemaking and bioethanol production. Little is known about gene expression or the physiology of I. orientalis under ethanol stress. In this study, high-throughput RNA sequencing was used to investigate the transcriptome profile of I. orientalis in response to ethanol. 502 gene transcripts were differentially expressed, of which 451 were more abundant, and 51 less abundant, in cells subjected to 4 h of ethanol stress (10% v/v). Annotation and statistical analyses suggest that multiple genes involved in ergosterol biosynthesis, trehalose metabolism, and stress response are differentially expressed under these conditions. The up-regulation of molecular chaperones HSP90 and HSP70, and also genes associated with the ubiquitin–proteasome proteolytic pathway suggests that ethanol stress may cause aggregation of misfolded proteins. Finally, ethanol stress in I. orientalis appears to have a nitrogen starvation effect, and many genes involved in nutrient uptake were up-regulated. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13568-018-0568-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-58497082018-03-21 Transcriptome profiling of Issatchenkia orientalis under ethanol stress Miao, Yingjie Xiong, Guotong Li, Ruoyun Wu, Zufang Zhang, Xin Weng, Peifang AMB Express Original Article Issatchenkia orientalis, a non-Saccharomyces yeast that can resist a wide variety of environmental stresses, has potential use in winemaking and bioethanol production. Little is known about gene expression or the physiology of I. orientalis under ethanol stress. In this study, high-throughput RNA sequencing was used to investigate the transcriptome profile of I. orientalis in response to ethanol. 502 gene transcripts were differentially expressed, of which 451 were more abundant, and 51 less abundant, in cells subjected to 4 h of ethanol stress (10% v/v). Annotation and statistical analyses suggest that multiple genes involved in ergosterol biosynthesis, trehalose metabolism, and stress response are differentially expressed under these conditions. The up-regulation of molecular chaperones HSP90 and HSP70, and also genes associated with the ubiquitin–proteasome proteolytic pathway suggests that ethanol stress may cause aggregation of misfolded proteins. Finally, ethanol stress in I. orientalis appears to have a nitrogen starvation effect, and many genes involved in nutrient uptake were up-regulated. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13568-018-0568-5) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2018-03-13 /pmc/articles/PMC5849708/ /pubmed/29536208 http://dx.doi.org/10.1186/s13568-018-0568-5 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Miao, Yingjie
Xiong, Guotong
Li, Ruoyun
Wu, Zufang
Zhang, Xin
Weng, Peifang
Transcriptome profiling of Issatchenkia orientalis under ethanol stress
title Transcriptome profiling of Issatchenkia orientalis under ethanol stress
title_full Transcriptome profiling of Issatchenkia orientalis under ethanol stress
title_fullStr Transcriptome profiling of Issatchenkia orientalis under ethanol stress
title_full_unstemmed Transcriptome profiling of Issatchenkia orientalis under ethanol stress
title_short Transcriptome profiling of Issatchenkia orientalis under ethanol stress
title_sort transcriptome profiling of issatchenkia orientalis under ethanol stress
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5849708/
https://www.ncbi.nlm.nih.gov/pubmed/29536208
http://dx.doi.org/10.1186/s13568-018-0568-5
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