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The dynamics of TGF-β in dental pulp, odontoblasts and dentin

Transforming growth factor-beta (TGF-β) is critical for cell proliferation and differentiation in dental pulp. Here, we show the dynamic mechanisms of TGF-β in porcine dental pulp, odontoblasts and dentin. The mRNA of latent TGF-β1 and TGF-β3 is predominantly expressed in odontoblasts, whereas the m...

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Autores principales: Niwa, Takahiko, Yamakoshi, Yasuo, Yamazaki, Hajime, Karakida, Takeo, Chiba, Risako, Hu, Jan C.-C., Nagano, Takatoshi, Yamamoto, Ryuji, Simmer, James P., Margolis, Henry C., Gomi, Kazuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5849713/
https://www.ncbi.nlm.nih.gov/pubmed/29535349
http://dx.doi.org/10.1038/s41598-018-22823-7
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author Niwa, Takahiko
Yamakoshi, Yasuo
Yamazaki, Hajime
Karakida, Takeo
Chiba, Risako
Hu, Jan C.-C.
Nagano, Takatoshi
Yamamoto, Ryuji
Simmer, James P.
Margolis, Henry C.
Gomi, Kazuhiro
author_facet Niwa, Takahiko
Yamakoshi, Yasuo
Yamazaki, Hajime
Karakida, Takeo
Chiba, Risako
Hu, Jan C.-C.
Nagano, Takatoshi
Yamamoto, Ryuji
Simmer, James P.
Margolis, Henry C.
Gomi, Kazuhiro
author_sort Niwa, Takahiko
collection PubMed
description Transforming growth factor-beta (TGF-β) is critical for cell proliferation and differentiation in dental pulp. Here, we show the dynamic mechanisms of TGF-β in porcine dental pulp, odontoblasts and dentin. The mRNA of latent TGF-β1 and TGF-β3 is predominantly expressed in odontoblasts, whereas the mRNA expression level of latent TGF-β2 is high in dental pulp. TGF-β1 is a major isoform of TGF-β, and latent TGF-β1, synthesized in dental pulp, is primarily activated by matrix metalloproteinase 11 (MMP11). Activated TGF-β1 enhances the mRNA expression levels of MMP20 and full-length dentin sialophosphoprotein (DSPP) in dental pulp cells, coinciding with the induction of odontoblast differentiation. Latent TGF-β1 synthesized in odontoblasts is primarily activated by MMP2 and MMP20 in both odontoblasts and dentin. The activity level of TGF-β1 was reduced in the dentin of MMP20 null mice, although the amount of latent TGF-β1 expression did not change between wild-type and MMP20 null mice. TGF-β1 activity was reduced with the degradation of DSPP-derived proteins that occurs with ageing. We propose that to exert its multiple biological functions, TGF-β1 is involved in a complicated dynamic interaction with matrix metalloproteinases (MMPs) and/or DSPP-derived proteins present in dental pulp, odontoblasts and dentin.
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spelling pubmed-58497132018-03-21 The dynamics of TGF-β in dental pulp, odontoblasts and dentin Niwa, Takahiko Yamakoshi, Yasuo Yamazaki, Hajime Karakida, Takeo Chiba, Risako Hu, Jan C.-C. Nagano, Takatoshi Yamamoto, Ryuji Simmer, James P. Margolis, Henry C. Gomi, Kazuhiro Sci Rep Article Transforming growth factor-beta (TGF-β) is critical for cell proliferation and differentiation in dental pulp. Here, we show the dynamic mechanisms of TGF-β in porcine dental pulp, odontoblasts and dentin. The mRNA of latent TGF-β1 and TGF-β3 is predominantly expressed in odontoblasts, whereas the mRNA expression level of latent TGF-β2 is high in dental pulp. TGF-β1 is a major isoform of TGF-β, and latent TGF-β1, synthesized in dental pulp, is primarily activated by matrix metalloproteinase 11 (MMP11). Activated TGF-β1 enhances the mRNA expression levels of MMP20 and full-length dentin sialophosphoprotein (DSPP) in dental pulp cells, coinciding with the induction of odontoblast differentiation. Latent TGF-β1 synthesized in odontoblasts is primarily activated by MMP2 and MMP20 in both odontoblasts and dentin. The activity level of TGF-β1 was reduced in the dentin of MMP20 null mice, although the amount of latent TGF-β1 expression did not change between wild-type and MMP20 null mice. TGF-β1 activity was reduced with the degradation of DSPP-derived proteins that occurs with ageing. We propose that to exert its multiple biological functions, TGF-β1 is involved in a complicated dynamic interaction with matrix metalloproteinases (MMPs) and/or DSPP-derived proteins present in dental pulp, odontoblasts and dentin. Nature Publishing Group UK 2018-03-13 /pmc/articles/PMC5849713/ /pubmed/29535349 http://dx.doi.org/10.1038/s41598-018-22823-7 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Niwa, Takahiko
Yamakoshi, Yasuo
Yamazaki, Hajime
Karakida, Takeo
Chiba, Risako
Hu, Jan C.-C.
Nagano, Takatoshi
Yamamoto, Ryuji
Simmer, James P.
Margolis, Henry C.
Gomi, Kazuhiro
The dynamics of TGF-β in dental pulp, odontoblasts and dentin
title The dynamics of TGF-β in dental pulp, odontoblasts and dentin
title_full The dynamics of TGF-β in dental pulp, odontoblasts and dentin
title_fullStr The dynamics of TGF-β in dental pulp, odontoblasts and dentin
title_full_unstemmed The dynamics of TGF-β in dental pulp, odontoblasts and dentin
title_short The dynamics of TGF-β in dental pulp, odontoblasts and dentin
title_sort dynamics of tgf-β in dental pulp, odontoblasts and dentin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5849713/
https://www.ncbi.nlm.nih.gov/pubmed/29535349
http://dx.doi.org/10.1038/s41598-018-22823-7
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