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Genomic characterisation of Leptospira inadai serogroup Lyme isolated from captured rat in Brazil and comparative analysis with human reference strain

Leptospira inadai is classified as a species of the Leptospira intermediate group that has been poorly studied due to its apparent insignificance to human and animal health. Nevertheless, over the last two decades the species has been described in human cases in India and in carrier animals in Ecuad...

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Autores principales: Moreno, Luisa Z, Miraglia, Fabiana, Loureiro, Ana P, Kremer, Frederico S, Eslabao, Marcus R, Dellagostin, Odir A, Lilenbaum, Walter, Vasconcellos, Silvio A, Heinemann, Marcos B, Moreno, Andrea M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5851024/
https://www.ncbi.nlm.nih.gov/pubmed/29538491
http://dx.doi.org/10.1590/0074-02760170444
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author Moreno, Luisa Z
Miraglia, Fabiana
Loureiro, Ana P
Kremer, Frederico S
Eslabao, Marcus R
Dellagostin, Odir A
Lilenbaum, Walter
Vasconcellos, Silvio A
Heinemann, Marcos B
Moreno, Andrea M
author_facet Moreno, Luisa Z
Miraglia, Fabiana
Loureiro, Ana P
Kremer, Frederico S
Eslabao, Marcus R
Dellagostin, Odir A
Lilenbaum, Walter
Vasconcellos, Silvio A
Heinemann, Marcos B
Moreno, Andrea M
author_sort Moreno, Luisa Z
collection PubMed
description Leptospira inadai is classified as a species of the Leptospira intermediate group that has been poorly studied due to its apparent insignificance to human and animal health. Nevertheless, over the last two decades the species has been described in human cases in India and in carrier animals in Ecuador. Here, we present the first identification and genomic characterisation of L. inadai serogroup Lyme isolated from captured rodent in Brazil. Even though the M34/99 strain was not pathogenic for hamsters, it was able to establish renal colonisation. The M34/99 strain presented high similarity with L. inadai serogroup Lyme human reference indicating that animal strain could also infect humans, although it does not represent high risk of severe disease. An extrachromosomal sequence was also identified in M34/99 strain and presented high identity with previously described L. inadai phage LinZ_10, suggesting that phage-like extrachromosomal sequence may be another feature of this understudied species.
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spelling pubmed-58510242018-03-20 Genomic characterisation of Leptospira inadai serogroup Lyme isolated from captured rat in Brazil and comparative analysis with human reference strain Moreno, Luisa Z Miraglia, Fabiana Loureiro, Ana P Kremer, Frederico S Eslabao, Marcus R Dellagostin, Odir A Lilenbaum, Walter Vasconcellos, Silvio A Heinemann, Marcos B Moreno, Andrea M Mem Inst Oswaldo Cruz Genome Announcements and Highlights Leptospira inadai is classified as a species of the Leptospira intermediate group that has been poorly studied due to its apparent insignificance to human and animal health. Nevertheless, over the last two decades the species has been described in human cases in India and in carrier animals in Ecuador. Here, we present the first identification and genomic characterisation of L. inadai serogroup Lyme isolated from captured rodent in Brazil. Even though the M34/99 strain was not pathogenic for hamsters, it was able to establish renal colonisation. The M34/99 strain presented high similarity with L. inadai serogroup Lyme human reference indicating that animal strain could also infect humans, although it does not represent high risk of severe disease. An extrachromosomal sequence was also identified in M34/99 strain and presented high identity with previously described L. inadai phage LinZ_10, suggesting that phage-like extrachromosomal sequence may be another feature of this understudied species. Instituto Oswaldo Cruz, Ministério da Saúde 2018-03-12 /pmc/articles/PMC5851024/ /pubmed/29538491 http://dx.doi.org/10.1590/0074-02760170444 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Genome Announcements and Highlights
Moreno, Luisa Z
Miraglia, Fabiana
Loureiro, Ana P
Kremer, Frederico S
Eslabao, Marcus R
Dellagostin, Odir A
Lilenbaum, Walter
Vasconcellos, Silvio A
Heinemann, Marcos B
Moreno, Andrea M
Genomic characterisation of Leptospira inadai serogroup Lyme isolated from captured rat in Brazil and comparative analysis with human reference strain
title Genomic characterisation of Leptospira inadai serogroup Lyme isolated from captured rat in Brazil and comparative analysis with human reference strain
title_full Genomic characterisation of Leptospira inadai serogroup Lyme isolated from captured rat in Brazil and comparative analysis with human reference strain
title_fullStr Genomic characterisation of Leptospira inadai serogroup Lyme isolated from captured rat in Brazil and comparative analysis with human reference strain
title_full_unstemmed Genomic characterisation of Leptospira inadai serogroup Lyme isolated from captured rat in Brazil and comparative analysis with human reference strain
title_short Genomic characterisation of Leptospira inadai serogroup Lyme isolated from captured rat in Brazil and comparative analysis with human reference strain
title_sort genomic characterisation of leptospira inadai serogroup lyme isolated from captured rat in brazil and comparative analysis with human reference strain
topic Genome Announcements and Highlights
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5851024/
https://www.ncbi.nlm.nih.gov/pubmed/29538491
http://dx.doi.org/10.1590/0074-02760170444
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