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Putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study

BACKGROUND: Salivary protein biomarkers for screening and diagnosis of oral lichen planus (OLP) are not well-defined. The objective of this study was to identify putative protein biomarkers for OLP using proteomic approaches. METHODS: Pooled unstimulated whole saliva was collected from five OLP pati...

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Autores principales: Talungchit, Sineepat, Buajeeb, Waranun, Lerdtripop, Chotima, Surarit, Rudee, Chairatvit, Kongthawat, Roytrakul, Sittiruk, Kobayashi, Hiroaki, Izumi, Yuichi, Khovidhunkit, Siribang-on Piboonniyom
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5851270/
https://www.ncbi.nlm.nih.gov/pubmed/29534707
http://dx.doi.org/10.1186/s12903-018-0504-8
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author Talungchit, Sineepat
Buajeeb, Waranun
Lerdtripop, Chotima
Surarit, Rudee
Chairatvit, Kongthawat
Roytrakul, Sittiruk
Kobayashi, Hiroaki
Izumi, Yuichi
Khovidhunkit, Siribang-on Piboonniyom
author_facet Talungchit, Sineepat
Buajeeb, Waranun
Lerdtripop, Chotima
Surarit, Rudee
Chairatvit, Kongthawat
Roytrakul, Sittiruk
Kobayashi, Hiroaki
Izumi, Yuichi
Khovidhunkit, Siribang-on Piboonniyom
author_sort Talungchit, Sineepat
collection PubMed
description BACKGROUND: Salivary protein biomarkers for screening and diagnosis of oral lichen planus (OLP) are not well-defined. The objective of this study was to identify putative protein biomarkers for OLP using proteomic approaches. METHODS: Pooled unstimulated whole saliva was collected from five OLP patients and five healthy control participants. Saliva samples were then subjected to two-dimensional gel electrophoresis, followed by mass spectrometry to identify putative protein biomarkers. Subsequently, a subset of these putative biomarkers were validated in 24 OLP patients and 24 age-matched healthy control subjects, using an enzyme-linked immunosorbent assay (ELISA). Immunoblotting analyses were then performed in 3 pairs of age- and sex-matched OLP patients and healthy controls to confirm results from the ELISA study. RESULTS: Thirty-one protein spots were identified, corresponding to 20 unique proteins. Notably, fibrinogen fragment D and complement component C3c exhibited increased expression in OLP patients, while cystatin SA exhibited decreased expression in OLP patients, compared with healthy control subjects. ELISA analyses indicated increased expression of fibrinogen fragment D and complement component C3c, and decreased expression of cystatin SA, in the saliva of OLP patients. Statistical differences in the expression of salivary complement C3c were observed between OLP patients and healthy control subjects. Immunoblotting analyses confirmed the results of our ELISA study. CONCLUSION: Complement C3c, fibrinogen fragment D and cystatin SA may serve as salivary biomarkers for screening and/or diagnosis of OLP.
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spelling pubmed-58512702018-03-21 Putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study Talungchit, Sineepat Buajeeb, Waranun Lerdtripop, Chotima Surarit, Rudee Chairatvit, Kongthawat Roytrakul, Sittiruk Kobayashi, Hiroaki Izumi, Yuichi Khovidhunkit, Siribang-on Piboonniyom BMC Oral Health Research Article BACKGROUND: Salivary protein biomarkers for screening and diagnosis of oral lichen planus (OLP) are not well-defined. The objective of this study was to identify putative protein biomarkers for OLP using proteomic approaches. METHODS: Pooled unstimulated whole saliva was collected from five OLP patients and five healthy control participants. Saliva samples were then subjected to two-dimensional gel electrophoresis, followed by mass spectrometry to identify putative protein biomarkers. Subsequently, a subset of these putative biomarkers were validated in 24 OLP patients and 24 age-matched healthy control subjects, using an enzyme-linked immunosorbent assay (ELISA). Immunoblotting analyses were then performed in 3 pairs of age- and sex-matched OLP patients and healthy controls to confirm results from the ELISA study. RESULTS: Thirty-one protein spots were identified, corresponding to 20 unique proteins. Notably, fibrinogen fragment D and complement component C3c exhibited increased expression in OLP patients, while cystatin SA exhibited decreased expression in OLP patients, compared with healthy control subjects. ELISA analyses indicated increased expression of fibrinogen fragment D and complement component C3c, and decreased expression of cystatin SA, in the saliva of OLP patients. Statistical differences in the expression of salivary complement C3c were observed between OLP patients and healthy control subjects. Immunoblotting analyses confirmed the results of our ELISA study. CONCLUSION: Complement C3c, fibrinogen fragment D and cystatin SA may serve as salivary biomarkers for screening and/or diagnosis of OLP. BioMed Central 2018-03-13 /pmc/articles/PMC5851270/ /pubmed/29534707 http://dx.doi.org/10.1186/s12903-018-0504-8 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Talungchit, Sineepat
Buajeeb, Waranun
Lerdtripop, Chotima
Surarit, Rudee
Chairatvit, Kongthawat
Roytrakul, Sittiruk
Kobayashi, Hiroaki
Izumi, Yuichi
Khovidhunkit, Siribang-on Piboonniyom
Putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study
title Putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study
title_full Putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study
title_fullStr Putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study
title_full_unstemmed Putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study
title_short Putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study
title_sort putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5851270/
https://www.ncbi.nlm.nih.gov/pubmed/29534707
http://dx.doi.org/10.1186/s12903-018-0504-8
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