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Putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study
BACKGROUND: Salivary protein biomarkers for screening and diagnosis of oral lichen planus (OLP) are not well-defined. The objective of this study was to identify putative protein biomarkers for OLP using proteomic approaches. METHODS: Pooled unstimulated whole saliva was collected from five OLP pati...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5851270/ https://www.ncbi.nlm.nih.gov/pubmed/29534707 http://dx.doi.org/10.1186/s12903-018-0504-8 |
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author | Talungchit, Sineepat Buajeeb, Waranun Lerdtripop, Chotima Surarit, Rudee Chairatvit, Kongthawat Roytrakul, Sittiruk Kobayashi, Hiroaki Izumi, Yuichi Khovidhunkit, Siribang-on Piboonniyom |
author_facet | Talungchit, Sineepat Buajeeb, Waranun Lerdtripop, Chotima Surarit, Rudee Chairatvit, Kongthawat Roytrakul, Sittiruk Kobayashi, Hiroaki Izumi, Yuichi Khovidhunkit, Siribang-on Piboonniyom |
author_sort | Talungchit, Sineepat |
collection | PubMed |
description | BACKGROUND: Salivary protein biomarkers for screening and diagnosis of oral lichen planus (OLP) are not well-defined. The objective of this study was to identify putative protein biomarkers for OLP using proteomic approaches. METHODS: Pooled unstimulated whole saliva was collected from five OLP patients and five healthy control participants. Saliva samples were then subjected to two-dimensional gel electrophoresis, followed by mass spectrometry to identify putative protein biomarkers. Subsequently, a subset of these putative biomarkers were validated in 24 OLP patients and 24 age-matched healthy control subjects, using an enzyme-linked immunosorbent assay (ELISA). Immunoblotting analyses were then performed in 3 pairs of age- and sex-matched OLP patients and healthy controls to confirm results from the ELISA study. RESULTS: Thirty-one protein spots were identified, corresponding to 20 unique proteins. Notably, fibrinogen fragment D and complement component C3c exhibited increased expression in OLP patients, while cystatin SA exhibited decreased expression in OLP patients, compared with healthy control subjects. ELISA analyses indicated increased expression of fibrinogen fragment D and complement component C3c, and decreased expression of cystatin SA, in the saliva of OLP patients. Statistical differences in the expression of salivary complement C3c were observed between OLP patients and healthy control subjects. Immunoblotting analyses confirmed the results of our ELISA study. CONCLUSION: Complement C3c, fibrinogen fragment D and cystatin SA may serve as salivary biomarkers for screening and/or diagnosis of OLP. |
format | Online Article Text |
id | pubmed-5851270 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-58512702018-03-21 Putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study Talungchit, Sineepat Buajeeb, Waranun Lerdtripop, Chotima Surarit, Rudee Chairatvit, Kongthawat Roytrakul, Sittiruk Kobayashi, Hiroaki Izumi, Yuichi Khovidhunkit, Siribang-on Piboonniyom BMC Oral Health Research Article BACKGROUND: Salivary protein biomarkers for screening and diagnosis of oral lichen planus (OLP) are not well-defined. The objective of this study was to identify putative protein biomarkers for OLP using proteomic approaches. METHODS: Pooled unstimulated whole saliva was collected from five OLP patients and five healthy control participants. Saliva samples were then subjected to two-dimensional gel electrophoresis, followed by mass spectrometry to identify putative protein biomarkers. Subsequently, a subset of these putative biomarkers were validated in 24 OLP patients and 24 age-matched healthy control subjects, using an enzyme-linked immunosorbent assay (ELISA). Immunoblotting analyses were then performed in 3 pairs of age- and sex-matched OLP patients and healthy controls to confirm results from the ELISA study. RESULTS: Thirty-one protein spots were identified, corresponding to 20 unique proteins. Notably, fibrinogen fragment D and complement component C3c exhibited increased expression in OLP patients, while cystatin SA exhibited decreased expression in OLP patients, compared with healthy control subjects. ELISA analyses indicated increased expression of fibrinogen fragment D and complement component C3c, and decreased expression of cystatin SA, in the saliva of OLP patients. Statistical differences in the expression of salivary complement C3c were observed between OLP patients and healthy control subjects. Immunoblotting analyses confirmed the results of our ELISA study. CONCLUSION: Complement C3c, fibrinogen fragment D and cystatin SA may serve as salivary biomarkers for screening and/or diagnosis of OLP. BioMed Central 2018-03-13 /pmc/articles/PMC5851270/ /pubmed/29534707 http://dx.doi.org/10.1186/s12903-018-0504-8 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Talungchit, Sineepat Buajeeb, Waranun Lerdtripop, Chotima Surarit, Rudee Chairatvit, Kongthawat Roytrakul, Sittiruk Kobayashi, Hiroaki Izumi, Yuichi Khovidhunkit, Siribang-on Piboonniyom Putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study |
title | Putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study |
title_full | Putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study |
title_fullStr | Putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study |
title_full_unstemmed | Putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study |
title_short | Putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study |
title_sort | putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5851270/ https://www.ncbi.nlm.nih.gov/pubmed/29534707 http://dx.doi.org/10.1186/s12903-018-0504-8 |
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