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The phorbol 12-myristate-13-acetate differentiation protocol is critical to the interaction of THP-1 macrophages with Salmonella Typhimurium
THP-1 cells differentiated with phorbol 12-myristate 13-acetate (PMA) are widely used as a model for function and biology of human macrophages. However, the conditions used for differentiation, particularly the concentration of PMA and the duration of treatment, vary widely. Here we compare several...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5851575/ https://www.ncbi.nlm.nih.gov/pubmed/29538403 http://dx.doi.org/10.1371/journal.pone.0193601 |
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author | Starr, Tregei Bauler, Timothy J. Malik-Kale, Preeti Steele-Mortimer, Olivia |
author_facet | Starr, Tregei Bauler, Timothy J. Malik-Kale, Preeti Steele-Mortimer, Olivia |
author_sort | Starr, Tregei |
collection | PubMed |
description | THP-1 cells differentiated with phorbol 12-myristate 13-acetate (PMA) are widely used as a model for function and biology of human macrophages. However, the conditions used for differentiation, particularly the concentration of PMA and the duration of treatment, vary widely. Here we compare several differentiation conditions and compare the ability of THP-1 macrophages to interact with the facultative intracellular pathogen Salmonella enterica serovar Typhimurium. The results show that THP-1 macrophages differentiated in high concentrations of PMA rapidly died following infection whereas those differentiated in low concentrations of PMA survived and were able to control the intracellular bacteria similar to primary human macrophages. |
format | Online Article Text |
id | pubmed-5851575 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58515752018-03-23 The phorbol 12-myristate-13-acetate differentiation protocol is critical to the interaction of THP-1 macrophages with Salmonella Typhimurium Starr, Tregei Bauler, Timothy J. Malik-Kale, Preeti Steele-Mortimer, Olivia PLoS One Research Article THP-1 cells differentiated with phorbol 12-myristate 13-acetate (PMA) are widely used as a model for function and biology of human macrophages. However, the conditions used for differentiation, particularly the concentration of PMA and the duration of treatment, vary widely. Here we compare several differentiation conditions and compare the ability of THP-1 macrophages to interact with the facultative intracellular pathogen Salmonella enterica serovar Typhimurium. The results show that THP-1 macrophages differentiated in high concentrations of PMA rapidly died following infection whereas those differentiated in low concentrations of PMA survived and were able to control the intracellular bacteria similar to primary human macrophages. Public Library of Science 2018-03-14 /pmc/articles/PMC5851575/ /pubmed/29538403 http://dx.doi.org/10.1371/journal.pone.0193601 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Starr, Tregei Bauler, Timothy J. Malik-Kale, Preeti Steele-Mortimer, Olivia The phorbol 12-myristate-13-acetate differentiation protocol is critical to the interaction of THP-1 macrophages with Salmonella Typhimurium |
title | The phorbol 12-myristate-13-acetate differentiation protocol is critical to the interaction of THP-1 macrophages with Salmonella Typhimurium |
title_full | The phorbol 12-myristate-13-acetate differentiation protocol is critical to the interaction of THP-1 macrophages with Salmonella Typhimurium |
title_fullStr | The phorbol 12-myristate-13-acetate differentiation protocol is critical to the interaction of THP-1 macrophages with Salmonella Typhimurium |
title_full_unstemmed | The phorbol 12-myristate-13-acetate differentiation protocol is critical to the interaction of THP-1 macrophages with Salmonella Typhimurium |
title_short | The phorbol 12-myristate-13-acetate differentiation protocol is critical to the interaction of THP-1 macrophages with Salmonella Typhimurium |
title_sort | phorbol 12-myristate-13-acetate differentiation protocol is critical to the interaction of thp-1 macrophages with salmonella typhimurium |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5851575/ https://www.ncbi.nlm.nih.gov/pubmed/29538403 http://dx.doi.org/10.1371/journal.pone.0193601 |
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