Rock signaling control PPARγ expression and actin polymerization during adipogenesis

Aim: Adipogenesis is characterized by a strong interdependence between cell shape, cytoskeletal organization, and the onset of adipogenic gene expression. Here we investigated the role of the RhoA/ROCK pathway in adipogenesis. Result: High RhoA activity in the cell line C3H10T1/2 were generated (Named RhoA14V cells). Treatment of RhoA14V cells with Shield 1 following their differentiation into adipocytes resulted in the appearance of thick cortical actin filaments, and increased mRNA expression levels of RhoA, ROCK, p-MYPT1 and p-MLC, while PPARγ mRNA decreased. This resulted in decreased triglyceride synthesis and reduced expression of the adipogenic transcription factor PPARγ. These molecular changes were accompanied by reorganization of the actin cytoskeleton, during which ROCK signaling suppressed actin polymerization. Conclusion: ROCK signaling suppresses adipogenesis by controlling PPARγ expression and actin organization in adipocytes.