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High-mobility group box 1 facilitates migration of neural stem cells via receptor for advanced glycation end products signaling pathway

High-mobility group box 1 (HMGB1) facilitates neural stem cells (NSCs) proliferation and differentiation into neuronal linage. However, the effect of HMGB1 on NSCs migration is still elusive. The present study is to investigate the corelation between HMGB1 and NSCs migration and the potential mechan...

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Autores principales: Xue, Xin, Chen, Xingxing, Fan, Weili, Wang, Guan, Zhang, Liang, Chen, Zongfeng, Liu, Peng, Liu, Mingyong, Zhao, Jianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5852130/
https://www.ncbi.nlm.nih.gov/pubmed/29540727
http://dx.doi.org/10.1038/s41598-018-22672-4
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author Xue, Xin
Chen, Xingxing
Fan, Weili
Wang, Guan
Zhang, Liang
Chen, Zongfeng
Liu, Peng
Liu, Mingyong
Zhao, Jianhua
author_facet Xue, Xin
Chen, Xingxing
Fan, Weili
Wang, Guan
Zhang, Liang
Chen, Zongfeng
Liu, Peng
Liu, Mingyong
Zhao, Jianhua
author_sort Xue, Xin
collection PubMed
description High-mobility group box 1 (HMGB1) facilitates neural stem cells (NSCs) proliferation and differentiation into neuronal linage. However, the effect of HMGB1 on NSCs migration is still elusive. The present study is to investigate the corelation between HMGB1 and NSCs migration and the potential mechanism. The results indicated that 1 ng/ml HMGB1 promoted NSCs proliferation using CCK8 assays. Moreover, data showed that 1 ng/ml HMGB1 facilitated NSCs migration via filopodia formation using phase-contrast and transwell assays. Furthermore, 1 ng/ml HMGB1 upregulated the expression of RAGE, one of the HMGB1 receptor, using western blotting assays and immunofluorescence staining. In addition, 1 ng/ml HMGB1 increased the percentage of filopodia formation using phalloidin staining. Meanwhile, the enhanced migration effect could be abrogated by 50 nM FPS-ZM1, one of the RAGE antagonist, and RAGE-specific siRNA through immunofluorescence and phalloidin staining. Together, our data demonstrate that HMGB1/RAGE axis facilitates NSCs migration via promoting filopodia formation, which might serve as a candidate for central nervous system (CNS) injury treatment and/or a preconditioning method for NSCs implantation.
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spelling pubmed-58521302018-03-22 High-mobility group box 1 facilitates migration of neural stem cells via receptor for advanced glycation end products signaling pathway Xue, Xin Chen, Xingxing Fan, Weili Wang, Guan Zhang, Liang Chen, Zongfeng Liu, Peng Liu, Mingyong Zhao, Jianhua Sci Rep Article High-mobility group box 1 (HMGB1) facilitates neural stem cells (NSCs) proliferation and differentiation into neuronal linage. However, the effect of HMGB1 on NSCs migration is still elusive. The present study is to investigate the corelation between HMGB1 and NSCs migration and the potential mechanism. The results indicated that 1 ng/ml HMGB1 promoted NSCs proliferation using CCK8 assays. Moreover, data showed that 1 ng/ml HMGB1 facilitated NSCs migration via filopodia formation using phase-contrast and transwell assays. Furthermore, 1 ng/ml HMGB1 upregulated the expression of RAGE, one of the HMGB1 receptor, using western blotting assays and immunofluorescence staining. In addition, 1 ng/ml HMGB1 increased the percentage of filopodia formation using phalloidin staining. Meanwhile, the enhanced migration effect could be abrogated by 50 nM FPS-ZM1, one of the RAGE antagonist, and RAGE-specific siRNA through immunofluorescence and phalloidin staining. Together, our data demonstrate that HMGB1/RAGE axis facilitates NSCs migration via promoting filopodia formation, which might serve as a candidate for central nervous system (CNS) injury treatment and/or a preconditioning method for NSCs implantation. Nature Publishing Group UK 2018-03-14 /pmc/articles/PMC5852130/ /pubmed/29540727 http://dx.doi.org/10.1038/s41598-018-22672-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Xue, Xin
Chen, Xingxing
Fan, Weili
Wang, Guan
Zhang, Liang
Chen, Zongfeng
Liu, Peng
Liu, Mingyong
Zhao, Jianhua
High-mobility group box 1 facilitates migration of neural stem cells via receptor for advanced glycation end products signaling pathway
title High-mobility group box 1 facilitates migration of neural stem cells via receptor for advanced glycation end products signaling pathway
title_full High-mobility group box 1 facilitates migration of neural stem cells via receptor for advanced glycation end products signaling pathway
title_fullStr High-mobility group box 1 facilitates migration of neural stem cells via receptor for advanced glycation end products signaling pathway
title_full_unstemmed High-mobility group box 1 facilitates migration of neural stem cells via receptor for advanced glycation end products signaling pathway
title_short High-mobility group box 1 facilitates migration of neural stem cells via receptor for advanced glycation end products signaling pathway
title_sort high-mobility group box 1 facilitates migration of neural stem cells via receptor for advanced glycation end products signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5852130/
https://www.ncbi.nlm.nih.gov/pubmed/29540727
http://dx.doi.org/10.1038/s41598-018-22672-4
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