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Data on solubilization, identification, and thermal stability of human Presenilin-2
The data presented here are related to the research article entitled “Expression, purification, and preliminary characterization of human presenilin-2" [1]. Human Presenilin-2 is the catalytic subunit of γ-secretase and a possible calcium leakage channel (Kimberly et al., 2000; Tu et al., 2006)...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5852265/ https://www.ncbi.nlm.nih.gov/pubmed/29552611 http://dx.doi.org/10.1016/j.dib.2018.01.039 |
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author | Yang, Ge Yu, Kun Kubicek, Jan Labahn, Jörg |
author_facet | Yang, Ge Yu, Kun Kubicek, Jan Labahn, Jörg |
author_sort | Yang, Ge |
collection | PubMed |
description | The data presented here are related to the research article entitled “Expression, purification, and preliminary characterization of human presenilin-2" [1]. Human Presenilin-2 is the catalytic subunit of γ-secretase and a possible calcium leakage channel (Kimberly et al., 2000; Tu et al., 2006) [2], [3]. HisPS2 which was obtained by overexpression in E. coli strain C43 (DE3) was extracted by detergent solubilisation. The sample isolation efficiency by detergents and the protein identification by mass spectrometry and western blot are described. This data article describes the near and far UV circular dichroism measurements and the data deconvolution in terms of secondary structure at 4 and 98 °C. Also, a refolding spectrum is presented. The raw CD spectra used for deconvolution of the helix and stand segments and average length are deposited into Protein Circular Dichroism Data Bank with PCDDBid: CD0005962000 (4 °C far UV), CD0005963000 (98 °C far UV), CD0005964000 (back to 4 °C far UV) and CD0005965000 (4 °C near UV CD). |
format | Online Article Text |
id | pubmed-5852265 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-58522652018-03-16 Data on solubilization, identification, and thermal stability of human Presenilin-2 Yang, Ge Yu, Kun Kubicek, Jan Labahn, Jörg Data Brief Proteomics and Biochemistry The data presented here are related to the research article entitled “Expression, purification, and preliminary characterization of human presenilin-2" [1]. Human Presenilin-2 is the catalytic subunit of γ-secretase and a possible calcium leakage channel (Kimberly et al., 2000; Tu et al., 2006) [2], [3]. HisPS2 which was obtained by overexpression in E. coli strain C43 (DE3) was extracted by detergent solubilisation. The sample isolation efficiency by detergents and the protein identification by mass spectrometry and western blot are described. This data article describes the near and far UV circular dichroism measurements and the data deconvolution in terms of secondary structure at 4 and 98 °C. Also, a refolding spectrum is presented. The raw CD spectra used for deconvolution of the helix and stand segments and average length are deposited into Protein Circular Dichroism Data Bank with PCDDBid: CD0005962000 (4 °C far UV), CD0005963000 (98 °C far UV), CD0005964000 (back to 4 °C far UV) and CD0005965000 (4 °C near UV CD). Elsevier 2018-02-03 /pmc/articles/PMC5852265/ /pubmed/29552611 http://dx.doi.org/10.1016/j.dib.2018.01.039 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Proteomics and Biochemistry Yang, Ge Yu, Kun Kubicek, Jan Labahn, Jörg Data on solubilization, identification, and thermal stability of human Presenilin-2 |
title | Data on solubilization, identification, and thermal stability of human Presenilin-2 |
title_full | Data on solubilization, identification, and thermal stability of human Presenilin-2 |
title_fullStr | Data on solubilization, identification, and thermal stability of human Presenilin-2 |
title_full_unstemmed | Data on solubilization, identification, and thermal stability of human Presenilin-2 |
title_short | Data on solubilization, identification, and thermal stability of human Presenilin-2 |
title_sort | data on solubilization, identification, and thermal stability of human presenilin-2 |
topic | Proteomics and Biochemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5852265/ https://www.ncbi.nlm.nih.gov/pubmed/29552611 http://dx.doi.org/10.1016/j.dib.2018.01.039 |
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