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High expression of DJ‐1 promotes growth and invasion via the PTEN‐AKT pathway and predicts a poor prognosis in colorectal cancer

Cancer cell invasion and unlimited proliferation are key factors in patients with colorectal cancer (CRC). Increased protein deglycase DJ‐1 in cancer cells is known to promote tumor growth; however, its role in CRC progression is not well defined. In this study, we investigated 100 CRC patients with...

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Autores principales: Lin, Yong, Chen, Qian, Liu, Quan‐xing, Zhou, Dong, Lu, Xiao, Deng, Xu‐feng, Yang, Hua, Zheng, Hong, Qiu, Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5852339/
https://www.ncbi.nlm.nih.gov/pubmed/29441725
http://dx.doi.org/10.1002/cam4.1325
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author Lin, Yong
Chen, Qian
Liu, Quan‐xing
Zhou, Dong
Lu, Xiao
Deng, Xu‐feng
Yang, Hua
Zheng, Hong
Qiu, Yuan
author_facet Lin, Yong
Chen, Qian
Liu, Quan‐xing
Zhou, Dong
Lu, Xiao
Deng, Xu‐feng
Yang, Hua
Zheng, Hong
Qiu, Yuan
author_sort Lin, Yong
collection PubMed
description Cancer cell invasion and unlimited proliferation are key factors in patients with colorectal cancer (CRC). Increased protein deglycase DJ‐1 in cancer cells is known to promote tumor growth; however, its role in CRC progression is not well defined. In this study, we investigated 100 CRC patients with disease stages I–IV to determine whether DJ‐1 could serve as a prognostic biomarker in CRC. These results showed that DJ‐1 expression in CRC tissues was higher than that in normal colon tissues and was associated with the (Tumor Node Metastasis) TNM stage. CRC patients with low DJ‐1 expression had a longer overall survival than those with high expression, and multivariate and univariate analyses indicated that DJ‐1 expression was an independent prognostic factor for overall survival in CRC. Furthermore, DJ‐1 overexpression in two colon cancer cell lines, HCT116 and SW480, activated protein kinase AKT and downregulated tumor suppressor PTEN, whereas DJ‐1 knockdown upregulated PTEN expression and effectively suppressed CRC cell invasion and proliferation both in vitro and in vivo, revealing a mechanism underlying DJ‐1 pro‐oncogenic activity in CRC. Treatment of MK2206, the specific AKT inhibitor, significantly decreased DJ‐1‐mediated cell proliferation and mobility in vitro. Taken together, these results suggest that DJ‐1 may be a novel prognostic biomarker and potential therapeutic target in human CRC.
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spelling pubmed-58523392018-03-22 High expression of DJ‐1 promotes growth and invasion via the PTEN‐AKT pathway and predicts a poor prognosis in colorectal cancer Lin, Yong Chen, Qian Liu, Quan‐xing Zhou, Dong Lu, Xiao Deng, Xu‐feng Yang, Hua Zheng, Hong Qiu, Yuan Cancer Med Cancer Biology Cancer cell invasion and unlimited proliferation are key factors in patients with colorectal cancer (CRC). Increased protein deglycase DJ‐1 in cancer cells is known to promote tumor growth; however, its role in CRC progression is not well defined. In this study, we investigated 100 CRC patients with disease stages I–IV to determine whether DJ‐1 could serve as a prognostic biomarker in CRC. These results showed that DJ‐1 expression in CRC tissues was higher than that in normal colon tissues and was associated with the (Tumor Node Metastasis) TNM stage. CRC patients with low DJ‐1 expression had a longer overall survival than those with high expression, and multivariate and univariate analyses indicated that DJ‐1 expression was an independent prognostic factor for overall survival in CRC. Furthermore, DJ‐1 overexpression in two colon cancer cell lines, HCT116 and SW480, activated protein kinase AKT and downregulated tumor suppressor PTEN, whereas DJ‐1 knockdown upregulated PTEN expression and effectively suppressed CRC cell invasion and proliferation both in vitro and in vivo, revealing a mechanism underlying DJ‐1 pro‐oncogenic activity in CRC. Treatment of MK2206, the specific AKT inhibitor, significantly decreased DJ‐1‐mediated cell proliferation and mobility in vitro. Taken together, these results suggest that DJ‐1 may be a novel prognostic biomarker and potential therapeutic target in human CRC. John Wiley and Sons Inc. 2018-02-14 /pmc/articles/PMC5852339/ /pubmed/29441725 http://dx.doi.org/10.1002/cam4.1325 Text en © 2018 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Cancer Biology
Lin, Yong
Chen, Qian
Liu, Quan‐xing
Zhou, Dong
Lu, Xiao
Deng, Xu‐feng
Yang, Hua
Zheng, Hong
Qiu, Yuan
High expression of DJ‐1 promotes growth and invasion via the PTEN‐AKT pathway and predicts a poor prognosis in colorectal cancer
title High expression of DJ‐1 promotes growth and invasion via the PTEN‐AKT pathway and predicts a poor prognosis in colorectal cancer
title_full High expression of DJ‐1 promotes growth and invasion via the PTEN‐AKT pathway and predicts a poor prognosis in colorectal cancer
title_fullStr High expression of DJ‐1 promotes growth and invasion via the PTEN‐AKT pathway and predicts a poor prognosis in colorectal cancer
title_full_unstemmed High expression of DJ‐1 promotes growth and invasion via the PTEN‐AKT pathway and predicts a poor prognosis in colorectal cancer
title_short High expression of DJ‐1 promotes growth and invasion via the PTEN‐AKT pathway and predicts a poor prognosis in colorectal cancer
title_sort high expression of dj‐1 promotes growth and invasion via the pten‐akt pathway and predicts a poor prognosis in colorectal cancer
topic Cancer Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5852339/
https://www.ncbi.nlm.nih.gov/pubmed/29441725
http://dx.doi.org/10.1002/cam4.1325
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