Cargando…

The AMP-Activated Protein Kinase Homolog Snf1 Concerts Carbon Utilization, Conidia Production and the Biosynthesis of Secondary Metabolites in the Taxol-Producer Pestalotiopsis microspora

Highly conserved, the Snf1/AMPK is a central regulator of carbon metabolism and energy production in the eukaryotes. However, its function in filamentous fungi has not been well established. In this study, we reported functional characterization of Snf1/AMPK in the growth, development and secondary...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Dan, Li, Yingying, Wang, Haichuan, Wei, Dongsheng, Akhberdi, Oren, Liu, Yanjie, Xiang, Biyun, Hao, Xiaoran, Zhu, Xudong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5852555/
https://www.ncbi.nlm.nih.gov/pubmed/29364863
http://dx.doi.org/10.3390/genes9020059
_version_ 1783306592322584576
author Wang, Dan
Li, Yingying
Wang, Haichuan
Wei, Dongsheng
Akhberdi, Oren
Liu, Yanjie
Xiang, Biyun
Hao, Xiaoran
Zhu, Xudong
author_facet Wang, Dan
Li, Yingying
Wang, Haichuan
Wei, Dongsheng
Akhberdi, Oren
Liu, Yanjie
Xiang, Biyun
Hao, Xiaoran
Zhu, Xudong
author_sort Wang, Dan
collection PubMed
description Highly conserved, the Snf1/AMPK is a central regulator of carbon metabolism and energy production in the eukaryotes. However, its function in filamentous fungi has not been well established. In this study, we reported functional characterization of Snf1/AMPK in the growth, development and secondary metabolism in the filamentous fungus Pestalotiopsis microspora. By deletion of the yeast SNF1 homolog, we found that it regulated the utilization of carbon sources, e.g., sucrose, demonstrating a conserved function of this kinase in filamentous fungus. Importantly, several novel functions of SNF1 were unraveled. For instance, the deletion strain displayed remarkable retardation in vegetative growth and pigmentation and produced a diminished number of conidia, even in the presence of the primary carbon source glucose. Deletion of the gene caused damages in the cell wall as shown by its hypersensitivities to Calcofluor white and Congo red, suggesting a critical role of Snf1 in maintaining cell wall integrity. Furthermore, the mutant strain Δsnf1 was hypersensitive to stress, e.g., osmotic pressure (1 M sorbitol), drug G418 and heat shock, though the mechanism remains to be illustrated. Significantly, disruption of the gene altered the production of secondary metabolites. By high-performance liquid chromatography (HPLC) profiling, we found that Δsnf1 barely produced secondary metabolites, e.g., the known product pestalotiollide B. This study suggests that Snf1 is a key regulator in filamentous fungus Pestalotiopsis microspora concerting carbon metabolism and the filamentous growth, conidiation, cell wall integrity, stress tolerance and the biosynthesis of secondary metabolites.
format Online
Article
Text
id pubmed-5852555
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-58525552018-03-19 The AMP-Activated Protein Kinase Homolog Snf1 Concerts Carbon Utilization, Conidia Production and the Biosynthesis of Secondary Metabolites in the Taxol-Producer Pestalotiopsis microspora Wang, Dan Li, Yingying Wang, Haichuan Wei, Dongsheng Akhberdi, Oren Liu, Yanjie Xiang, Biyun Hao, Xiaoran Zhu, Xudong Genes (Basel) Article Highly conserved, the Snf1/AMPK is a central regulator of carbon metabolism and energy production in the eukaryotes. However, its function in filamentous fungi has not been well established. In this study, we reported functional characterization of Snf1/AMPK in the growth, development and secondary metabolism in the filamentous fungus Pestalotiopsis microspora. By deletion of the yeast SNF1 homolog, we found that it regulated the utilization of carbon sources, e.g., sucrose, demonstrating a conserved function of this kinase in filamentous fungus. Importantly, several novel functions of SNF1 were unraveled. For instance, the deletion strain displayed remarkable retardation in vegetative growth and pigmentation and produced a diminished number of conidia, even in the presence of the primary carbon source glucose. Deletion of the gene caused damages in the cell wall as shown by its hypersensitivities to Calcofluor white and Congo red, suggesting a critical role of Snf1 in maintaining cell wall integrity. Furthermore, the mutant strain Δsnf1 was hypersensitive to stress, e.g., osmotic pressure (1 M sorbitol), drug G418 and heat shock, though the mechanism remains to be illustrated. Significantly, disruption of the gene altered the production of secondary metabolites. By high-performance liquid chromatography (HPLC) profiling, we found that Δsnf1 barely produced secondary metabolites, e.g., the known product pestalotiollide B. This study suggests that Snf1 is a key regulator in filamentous fungus Pestalotiopsis microspora concerting carbon metabolism and the filamentous growth, conidiation, cell wall integrity, stress tolerance and the biosynthesis of secondary metabolites. MDPI 2018-01-24 /pmc/articles/PMC5852555/ /pubmed/29364863 http://dx.doi.org/10.3390/genes9020059 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Dan
Li, Yingying
Wang, Haichuan
Wei, Dongsheng
Akhberdi, Oren
Liu, Yanjie
Xiang, Biyun
Hao, Xiaoran
Zhu, Xudong
The AMP-Activated Protein Kinase Homolog Snf1 Concerts Carbon Utilization, Conidia Production and the Biosynthesis of Secondary Metabolites in the Taxol-Producer Pestalotiopsis microspora
title The AMP-Activated Protein Kinase Homolog Snf1 Concerts Carbon Utilization, Conidia Production and the Biosynthesis of Secondary Metabolites in the Taxol-Producer Pestalotiopsis microspora
title_full The AMP-Activated Protein Kinase Homolog Snf1 Concerts Carbon Utilization, Conidia Production and the Biosynthesis of Secondary Metabolites in the Taxol-Producer Pestalotiopsis microspora
title_fullStr The AMP-Activated Protein Kinase Homolog Snf1 Concerts Carbon Utilization, Conidia Production and the Biosynthesis of Secondary Metabolites in the Taxol-Producer Pestalotiopsis microspora
title_full_unstemmed The AMP-Activated Protein Kinase Homolog Snf1 Concerts Carbon Utilization, Conidia Production and the Biosynthesis of Secondary Metabolites in the Taxol-Producer Pestalotiopsis microspora
title_short The AMP-Activated Protein Kinase Homolog Snf1 Concerts Carbon Utilization, Conidia Production and the Biosynthesis of Secondary Metabolites in the Taxol-Producer Pestalotiopsis microspora
title_sort amp-activated protein kinase homolog snf1 concerts carbon utilization, conidia production and the biosynthesis of secondary metabolites in the taxol-producer pestalotiopsis microspora
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5852555/
https://www.ncbi.nlm.nih.gov/pubmed/29364863
http://dx.doi.org/10.3390/genes9020059
work_keys_str_mv AT wangdan theampactivatedproteinkinasehomologsnf1concertscarbonutilizationconidiaproductionandthebiosynthesisofsecondarymetabolitesinthetaxolproducerpestalotiopsismicrospora
AT liyingying theampactivatedproteinkinasehomologsnf1concertscarbonutilizationconidiaproductionandthebiosynthesisofsecondarymetabolitesinthetaxolproducerpestalotiopsismicrospora
AT wanghaichuan theampactivatedproteinkinasehomologsnf1concertscarbonutilizationconidiaproductionandthebiosynthesisofsecondarymetabolitesinthetaxolproducerpestalotiopsismicrospora
AT weidongsheng theampactivatedproteinkinasehomologsnf1concertscarbonutilizationconidiaproductionandthebiosynthesisofsecondarymetabolitesinthetaxolproducerpestalotiopsismicrospora
AT akhberdioren theampactivatedproteinkinasehomologsnf1concertscarbonutilizationconidiaproductionandthebiosynthesisofsecondarymetabolitesinthetaxolproducerpestalotiopsismicrospora
AT liuyanjie theampactivatedproteinkinasehomologsnf1concertscarbonutilizationconidiaproductionandthebiosynthesisofsecondarymetabolitesinthetaxolproducerpestalotiopsismicrospora
AT xiangbiyun theampactivatedproteinkinasehomologsnf1concertscarbonutilizationconidiaproductionandthebiosynthesisofsecondarymetabolitesinthetaxolproducerpestalotiopsismicrospora
AT haoxiaoran theampactivatedproteinkinasehomologsnf1concertscarbonutilizationconidiaproductionandthebiosynthesisofsecondarymetabolitesinthetaxolproducerpestalotiopsismicrospora
AT zhuxudong theampactivatedproteinkinasehomologsnf1concertscarbonutilizationconidiaproductionandthebiosynthesisofsecondarymetabolitesinthetaxolproducerpestalotiopsismicrospora
AT wangdan ampactivatedproteinkinasehomologsnf1concertscarbonutilizationconidiaproductionandthebiosynthesisofsecondarymetabolitesinthetaxolproducerpestalotiopsismicrospora
AT liyingying ampactivatedproteinkinasehomologsnf1concertscarbonutilizationconidiaproductionandthebiosynthesisofsecondarymetabolitesinthetaxolproducerpestalotiopsismicrospora
AT wanghaichuan ampactivatedproteinkinasehomologsnf1concertscarbonutilizationconidiaproductionandthebiosynthesisofsecondarymetabolitesinthetaxolproducerpestalotiopsismicrospora
AT weidongsheng ampactivatedproteinkinasehomologsnf1concertscarbonutilizationconidiaproductionandthebiosynthesisofsecondarymetabolitesinthetaxolproducerpestalotiopsismicrospora
AT akhberdioren ampactivatedproteinkinasehomologsnf1concertscarbonutilizationconidiaproductionandthebiosynthesisofsecondarymetabolitesinthetaxolproducerpestalotiopsismicrospora
AT liuyanjie ampactivatedproteinkinasehomologsnf1concertscarbonutilizationconidiaproductionandthebiosynthesisofsecondarymetabolitesinthetaxolproducerpestalotiopsismicrospora
AT xiangbiyun ampactivatedproteinkinasehomologsnf1concertscarbonutilizationconidiaproductionandthebiosynthesisofsecondarymetabolitesinthetaxolproducerpestalotiopsismicrospora
AT haoxiaoran ampactivatedproteinkinasehomologsnf1concertscarbonutilizationconidiaproductionandthebiosynthesisofsecondarymetabolitesinthetaxolproducerpestalotiopsismicrospora
AT zhuxudong ampactivatedproteinkinasehomologsnf1concertscarbonutilizationconidiaproductionandthebiosynthesisofsecondarymetabolitesinthetaxolproducerpestalotiopsismicrospora