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Embedded Disposable Functionalized Electrochemical Biosensor with a 3D-Printed Flow Cell for Detection of Hepatic Oval Cells (HOCs)

Hepatic oval cells (HOCs) are considered the progeny of the intrahepatic stem cells that are found in a small population in the liver after hepatocyte proliferation is inhibited. Due to their small number, isolation and capture of these cells constitute a challenging task for immunosensor technology...

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Autores principales: Damiati, Samar, Peacock, Martin, Leonhardt, Stefan, Damiati, Laila, Baghdadi, Mohammed A., Becker, Holger, Kodzius, Rimantas, Schuster, Bernhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5852585/
https://www.ncbi.nlm.nih.gov/pubmed/29443890
http://dx.doi.org/10.3390/genes9020089
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author Damiati, Samar
Peacock, Martin
Leonhardt, Stefan
Damiati, Laila
Baghdadi, Mohammed A.
Becker, Holger
Kodzius, Rimantas
Schuster, Bernhard
author_facet Damiati, Samar
Peacock, Martin
Leonhardt, Stefan
Damiati, Laila
Baghdadi, Mohammed A.
Becker, Holger
Kodzius, Rimantas
Schuster, Bernhard
author_sort Damiati, Samar
collection PubMed
description Hepatic oval cells (HOCs) are considered the progeny of the intrahepatic stem cells that are found in a small population in the liver after hepatocyte proliferation is inhibited. Due to their small number, isolation and capture of these cells constitute a challenging task for immunosensor technology. This work describes the development of a 3D-printed continuous flow system and exploits disposable screen-printed electrodes for the rapid detection of HOCs that over-express the OV6 marker on their membrane. Multiwall carbon nanotube (MWCNT) electrodes have a chitosan film that serves as a scaffold for the immobilization of oval cell marker antibodies (anti-OV6-Ab), which enhance the sensitivity of the biomarker and makes the designed sensor specific for oval cells. The developed sensor can be easily embedded into the 3D-printed flow cell to allow cells to be exposed continuously to the functionalized surface. The continuous flow is intended to increase capture of most of the target cells in the specimen. Contact angle measurements were performed to characterize the nature and quality of the modified sensor surface, and electrochemical measurements (cyclic voltammetry (CV) and square wave voltammetry (SWV)) were performed to confirm the efficiency and selectivity of the fabricated sensor to detect HOCs. The proposed method is valuable for capturing rare cells and could provide an effective tool for cancer diagnosis and detection.
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spelling pubmed-58525852018-03-19 Embedded Disposable Functionalized Electrochemical Biosensor with a 3D-Printed Flow Cell for Detection of Hepatic Oval Cells (HOCs) Damiati, Samar Peacock, Martin Leonhardt, Stefan Damiati, Laila Baghdadi, Mohammed A. Becker, Holger Kodzius, Rimantas Schuster, Bernhard Genes (Basel) Article Hepatic oval cells (HOCs) are considered the progeny of the intrahepatic stem cells that are found in a small population in the liver after hepatocyte proliferation is inhibited. Due to their small number, isolation and capture of these cells constitute a challenging task for immunosensor technology. This work describes the development of a 3D-printed continuous flow system and exploits disposable screen-printed electrodes for the rapid detection of HOCs that over-express the OV6 marker on their membrane. Multiwall carbon nanotube (MWCNT) electrodes have a chitosan film that serves as a scaffold for the immobilization of oval cell marker antibodies (anti-OV6-Ab), which enhance the sensitivity of the biomarker and makes the designed sensor specific for oval cells. The developed sensor can be easily embedded into the 3D-printed flow cell to allow cells to be exposed continuously to the functionalized surface. The continuous flow is intended to increase capture of most of the target cells in the specimen. Contact angle measurements were performed to characterize the nature and quality of the modified sensor surface, and electrochemical measurements (cyclic voltammetry (CV) and square wave voltammetry (SWV)) were performed to confirm the efficiency and selectivity of the fabricated sensor to detect HOCs. The proposed method is valuable for capturing rare cells and could provide an effective tool for cancer diagnosis and detection. MDPI 2018-02-14 /pmc/articles/PMC5852585/ /pubmed/29443890 http://dx.doi.org/10.3390/genes9020089 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Damiati, Samar
Peacock, Martin
Leonhardt, Stefan
Damiati, Laila
Baghdadi, Mohammed A.
Becker, Holger
Kodzius, Rimantas
Schuster, Bernhard
Embedded Disposable Functionalized Electrochemical Biosensor with a 3D-Printed Flow Cell for Detection of Hepatic Oval Cells (HOCs)
title Embedded Disposable Functionalized Electrochemical Biosensor with a 3D-Printed Flow Cell for Detection of Hepatic Oval Cells (HOCs)
title_full Embedded Disposable Functionalized Electrochemical Biosensor with a 3D-Printed Flow Cell for Detection of Hepatic Oval Cells (HOCs)
title_fullStr Embedded Disposable Functionalized Electrochemical Biosensor with a 3D-Printed Flow Cell for Detection of Hepatic Oval Cells (HOCs)
title_full_unstemmed Embedded Disposable Functionalized Electrochemical Biosensor with a 3D-Printed Flow Cell for Detection of Hepatic Oval Cells (HOCs)
title_short Embedded Disposable Functionalized Electrochemical Biosensor with a 3D-Printed Flow Cell for Detection of Hepatic Oval Cells (HOCs)
title_sort embedded disposable functionalized electrochemical biosensor with a 3d-printed flow cell for detection of hepatic oval cells (hocs)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5852585/
https://www.ncbi.nlm.nih.gov/pubmed/29443890
http://dx.doi.org/10.3390/genes9020089
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