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Impact of Western and Mediterranean Diets and Vitamin D on Muscle Fibers of Sedentary Rats

Background: The metabolic syndrome is associated with sarcopenia. Decreased serum levels of Vitamin D (VitD) and insulin-like growth factor (IGF)-1 and their mutual relationship were also reported. We aimed to evaluate whether different dietary profiles, containing or not VitD, may exert different e...

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Autores principales: Trovato, Francesca Maria, Castrogiovanni, Paola, Szychlinska, Marta Anna, Purrello, Francesco, Musumeci, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5852807/
https://www.ncbi.nlm.nih.gov/pubmed/29462978
http://dx.doi.org/10.3390/nu10020231
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author Trovato, Francesca Maria
Castrogiovanni, Paola
Szychlinska, Marta Anna
Purrello, Francesco
Musumeci, Giuseppe
author_facet Trovato, Francesca Maria
Castrogiovanni, Paola
Szychlinska, Marta Anna
Purrello, Francesco
Musumeci, Giuseppe
author_sort Trovato, Francesca Maria
collection PubMed
description Background: The metabolic syndrome is associated with sarcopenia. Decreased serum levels of Vitamin D (VitD) and insulin-like growth factor (IGF)-1 and their mutual relationship were also reported. We aimed to evaluate whether different dietary profiles, containing or not VitD, may exert different effects on muscle molecular morphology. Methods: Twenty-eight male rats were fed for 10 weeks in order to detect early defects induced by different dietary regimens: regular diet (R); regular diet with vitamin D supplementation (R-DS) and regular diet with vitamin D restriction (R-DR); high-fat butter-based diets (HFB-DS and HFB-DR) with 41% energy from fat; high-fat extra-virgin olive oil-based diets (HFEVO-DS and HFEVO-DR) with 41% energy from fat. IL-1β, insulin-like growth factor (IGF)1, Dickkopf-1 (DKK-1), and VitD-receptor (VDR) expressions were evaluated by immunohistochemistry. Muscle fiber perimeter was measured by histology and morphometric analysis. Results: The muscle fibers of the HEVO-DS rats were hypertrophic, comparable to those of the R-DS rats. An inverse correlation existed between the dietary fat content and the perimeter of the muscle fibers (p < 0.01). In the HFB-DR rats, the muscle fibers appeared hypotrophic with an increase of IL-1β and a dramatic decrease of IGF-1 expression. Conclusions: High-fat western diet could impair muscle metabolism and lay the ground for subsequent muscle damage. VitD associated with a Mediterranean diet showed trophic action on the muscle fibers.
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spelling pubmed-58528072018-03-19 Impact of Western and Mediterranean Diets and Vitamin D on Muscle Fibers of Sedentary Rats Trovato, Francesca Maria Castrogiovanni, Paola Szychlinska, Marta Anna Purrello, Francesco Musumeci, Giuseppe Nutrients Article Background: The metabolic syndrome is associated with sarcopenia. Decreased serum levels of Vitamin D (VitD) and insulin-like growth factor (IGF)-1 and their mutual relationship were also reported. We aimed to evaluate whether different dietary profiles, containing or not VitD, may exert different effects on muscle molecular morphology. Methods: Twenty-eight male rats were fed for 10 weeks in order to detect early defects induced by different dietary regimens: regular diet (R); regular diet with vitamin D supplementation (R-DS) and regular diet with vitamin D restriction (R-DR); high-fat butter-based diets (HFB-DS and HFB-DR) with 41% energy from fat; high-fat extra-virgin olive oil-based diets (HFEVO-DS and HFEVO-DR) with 41% energy from fat. IL-1β, insulin-like growth factor (IGF)1, Dickkopf-1 (DKK-1), and VitD-receptor (VDR) expressions were evaluated by immunohistochemistry. Muscle fiber perimeter was measured by histology and morphometric analysis. Results: The muscle fibers of the HEVO-DS rats were hypertrophic, comparable to those of the R-DS rats. An inverse correlation existed between the dietary fat content and the perimeter of the muscle fibers (p < 0.01). In the HFB-DR rats, the muscle fibers appeared hypotrophic with an increase of IL-1β and a dramatic decrease of IGF-1 expression. Conclusions: High-fat western diet could impair muscle metabolism and lay the ground for subsequent muscle damage. VitD associated with a Mediterranean diet showed trophic action on the muscle fibers. MDPI 2018-02-17 /pmc/articles/PMC5852807/ /pubmed/29462978 http://dx.doi.org/10.3390/nu10020231 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Trovato, Francesca Maria
Castrogiovanni, Paola
Szychlinska, Marta Anna
Purrello, Francesco
Musumeci, Giuseppe
Impact of Western and Mediterranean Diets and Vitamin D on Muscle Fibers of Sedentary Rats
title Impact of Western and Mediterranean Diets and Vitamin D on Muscle Fibers of Sedentary Rats
title_full Impact of Western and Mediterranean Diets and Vitamin D on Muscle Fibers of Sedentary Rats
title_fullStr Impact of Western and Mediterranean Diets and Vitamin D on Muscle Fibers of Sedentary Rats
title_full_unstemmed Impact of Western and Mediterranean Diets and Vitamin D on Muscle Fibers of Sedentary Rats
title_short Impact of Western and Mediterranean Diets and Vitamin D on Muscle Fibers of Sedentary Rats
title_sort impact of western and mediterranean diets and vitamin d on muscle fibers of sedentary rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5852807/
https://www.ncbi.nlm.nih.gov/pubmed/29462978
http://dx.doi.org/10.3390/nu10020231
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