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MADS-box family genes in sheepgrass and their involvement in abiotic stress responses
BACKGROUND: MADS-box genes are categorized into A, B, C, D and E classes and are involved in floral organ identity and flowering. Sheepgrass (Leymus chinensis (Trin.) Tzvel) is an important perennial forage grass and adapts well to many adverse environments. However, there are few studies on the mol...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853078/ https://www.ncbi.nlm.nih.gov/pubmed/29540194 http://dx.doi.org/10.1186/s12870-018-1259-8 |
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author | Jia, Junting Zhao, Pincang Cheng, Liqin Yuan, Guangxiao Yang, Weiguang Liu, Shu Chen, Shuangyan Qi, Dongmei Liu, Gongshe Li, Xiaoxia |
author_facet | Jia, Junting Zhao, Pincang Cheng, Liqin Yuan, Guangxiao Yang, Weiguang Liu, Shu Chen, Shuangyan Qi, Dongmei Liu, Gongshe Li, Xiaoxia |
author_sort | Jia, Junting |
collection | PubMed |
description | BACKGROUND: MADS-box genes are categorized into A, B, C, D and E classes and are involved in floral organ identity and flowering. Sheepgrass (Leymus chinensis (Trin.) Tzvel) is an important perennial forage grass and adapts well to many adverse environments. However, there are few studies on the molecular mechanisms of flower development in sheepgrass, especially studies on MADS-domain proteins. RESULTS: In this study, we cloned 11 MADS-box genes from sheepgrass (Leymus chinensis (Trin.) Tzvel), and phylogenetic analysis of the 11 genes with their homologs revealed that they are divided into nine subclades. Tissue-specific expression profile analysis showed that most of these MADS-box genes were highly expressed in floral organs. LcMADS1 and LcMADS3 showed higher expression in the stamen than in the other tissues, and LcMADS7 showed high expression in the stamen, glume, lemma and palea, while expression of LcMADS2, LcMADS9 and LcMADS11 was higher in vegetative organs than floral organs. Furthermore, yeast two-hybrid analyses showed that LcMADS2 interacted with LcMADS7 and LcMADS9. LcMADS3 interacted with LcMADS4, LcMADS7 and LcMADS10, while LcMADS1 could interact with only LcMADS7. Interestingly, the expression of LcMADS1 and LcMADS2 were significantly induced by cold, and LcMADS9 was significantly up-regulated by NaCl. CONCLUSION: Hence, we proposed that LcMADS1, LcMADS2, LcMADS3, LcMADS7 and LcMADS9 play a pivotal role in sheepgrass sexual reproduction and may be involved in abiotic stress responses, and our findings provide useful information for further exploration of the functions of this gene family in rice, wheat and other graminaceous cereals. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1259-8) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5853078 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-58530782018-03-22 MADS-box family genes in sheepgrass and their involvement in abiotic stress responses Jia, Junting Zhao, Pincang Cheng, Liqin Yuan, Guangxiao Yang, Weiguang Liu, Shu Chen, Shuangyan Qi, Dongmei Liu, Gongshe Li, Xiaoxia BMC Plant Biol Research Article BACKGROUND: MADS-box genes are categorized into A, B, C, D and E classes and are involved in floral organ identity and flowering. Sheepgrass (Leymus chinensis (Trin.) Tzvel) is an important perennial forage grass and adapts well to many adverse environments. However, there are few studies on the molecular mechanisms of flower development in sheepgrass, especially studies on MADS-domain proteins. RESULTS: In this study, we cloned 11 MADS-box genes from sheepgrass (Leymus chinensis (Trin.) Tzvel), and phylogenetic analysis of the 11 genes with their homologs revealed that they are divided into nine subclades. Tissue-specific expression profile analysis showed that most of these MADS-box genes were highly expressed in floral organs. LcMADS1 and LcMADS3 showed higher expression in the stamen than in the other tissues, and LcMADS7 showed high expression in the stamen, glume, lemma and palea, while expression of LcMADS2, LcMADS9 and LcMADS11 was higher in vegetative organs than floral organs. Furthermore, yeast two-hybrid analyses showed that LcMADS2 interacted with LcMADS7 and LcMADS9. LcMADS3 interacted with LcMADS4, LcMADS7 and LcMADS10, while LcMADS1 could interact with only LcMADS7. Interestingly, the expression of LcMADS1 and LcMADS2 were significantly induced by cold, and LcMADS9 was significantly up-regulated by NaCl. CONCLUSION: Hence, we proposed that LcMADS1, LcMADS2, LcMADS3, LcMADS7 and LcMADS9 play a pivotal role in sheepgrass sexual reproduction and may be involved in abiotic stress responses, and our findings provide useful information for further exploration of the functions of this gene family in rice, wheat and other graminaceous cereals. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1259-8) contains supplementary material, which is available to authorized users. BioMed Central 2018-03-14 /pmc/articles/PMC5853078/ /pubmed/29540194 http://dx.doi.org/10.1186/s12870-018-1259-8 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Jia, Junting Zhao, Pincang Cheng, Liqin Yuan, Guangxiao Yang, Weiguang Liu, Shu Chen, Shuangyan Qi, Dongmei Liu, Gongshe Li, Xiaoxia MADS-box family genes in sheepgrass and their involvement in abiotic stress responses |
title | MADS-box family genes in sheepgrass and their involvement in abiotic stress responses |
title_full | MADS-box family genes in sheepgrass and their involvement in abiotic stress responses |
title_fullStr | MADS-box family genes in sheepgrass and their involvement in abiotic stress responses |
title_full_unstemmed | MADS-box family genes in sheepgrass and their involvement in abiotic stress responses |
title_short | MADS-box family genes in sheepgrass and their involvement in abiotic stress responses |
title_sort | mads-box family genes in sheepgrass and their involvement in abiotic stress responses |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853078/ https://www.ncbi.nlm.nih.gov/pubmed/29540194 http://dx.doi.org/10.1186/s12870-018-1259-8 |
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