A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone

BACKGROUND: Clustered regularly interspaced short palindromic repeat (CRISPR) RNA-guided adaptive immune systems are found in prokaryotes to defend cells from foreign DNA. CRISPR Cas9 systems have been modified and employed as genome editing tools in wide ranging organisms. Here, we provide a detail...

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Detalles Bibliográficos
Autores principales: Vazquez, Neftali, Sanchez, Lilia, Marks, Rebecca, Martinez, Eduardo, Fanniel, Victor, Lopez, Alma, Salinas, Andrea, Flores, Itzel, Hirschmann, Jesse, Gilkerson, Robert, Schuenzel, Erin, Dearth, Robert, Halaby, Reginald, Innis-Whitehouse, Wendy, Keniry, Megan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853148/
https://www.ncbi.nlm.nih.gov/pubmed/29540148
http://dx.doi.org/10.1186/s12867-018-0105-8

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